Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses
Heat stress is a major concern for the growth of cool-season creeping bentgrass (<i>Agrostis stolonifera</i> L.). Nonetheless, there is a lack in a clear and systematic understanding of thermotolerance mechanisms for this species. This study aimed to assess heat tolerance in experimental...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/1/41 |
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| author | Qianqian Fan David Jespersen |
| author_facet | Qianqian Fan David Jespersen |
| author_sort | Qianqian Fan |
| collection | DOAJ |
| description | Heat stress is a major concern for the growth of cool-season creeping bentgrass (<i>Agrostis stolonifera</i> L.). Nonetheless, there is a lack in a clear and systematic understanding of thermotolerance mechanisms for this species. This study aimed to assess heat tolerance in experimental lines and cultivars to determine important physiological and biochemical traits responsible for improved tolerance, including the use of OJIP fluorescence. Ten creeping bentgrass lines were exposed to either control (20/15 °C day/night) or high temperature (38/33 °C day/night) conditions for 35 d via growth chambers at Griffin, GA. Principal component analysis and clustering analysis were performed to rank stress performance and divide lines into different groups according to their tolerance similarities, respectively. At the end of the trial, S11 729-10 and BTC032 were in the most thermotolerant group, followed by a group containing BTC011, AU Victory and Penncross. Crenshaw belonged to the most heat-sensitive group while S11 675-02 and Pure Eclipse were in the second most heat-sensitive group. The exceptional thermotolerance in S11 729-10 and BTC032 was associated with their abilities to maintain cell membrane stability and protein metabolism, plus minimize oxidative damages. Additionally, among various light-harvesting steps, energy trapping, dissipation and electron transport from Q<sub>A</sub> to PQ were more heat-sensitive than electron transport from Q<sub>A</sub> to final PSI acceptors. Along with the strong correlations between multiple OJIP parameters and other traits, it reveals that OJIP fluorescence could be a valuable tool for dissection of photosynthetic processes and identification of the critical steps responsible for photosynthetic declines, enabling a more targeted heat-stress screening. Our results indicated that variability in the level of heat tolerance and associated mechanisms in creeping bentgrass germplasm could be utilized to develop new cultivars with improved thermotolerance. |
| first_indexed | 2024-03-09T09:42:33Z |
| format | Article |
| id | doaj.art-56b21e6f118a4f8192b7cd122f6050be |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T09:42:33Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-56b21e6f118a4f8192b7cd122f6050be2023-12-02T00:47:17ZengMDPI AGPlants2223-77472022-12-011214110.3390/plants12010041Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological ResponsesQianqian Fan0David Jespersen1Department of Crop and Soil Sciences, University of Georgia, Griffin Campus, 1109 Experiment Street, Griffin, GA 30223, USADepartment of Crop and Soil Sciences, University of Georgia, Griffin Campus, 1109 Experiment Street, Griffin, GA 30223, USAHeat stress is a major concern for the growth of cool-season creeping bentgrass (<i>Agrostis stolonifera</i> L.). Nonetheless, there is a lack in a clear and systematic understanding of thermotolerance mechanisms for this species. This study aimed to assess heat tolerance in experimental lines and cultivars to determine important physiological and biochemical traits responsible for improved tolerance, including the use of OJIP fluorescence. Ten creeping bentgrass lines were exposed to either control (20/15 °C day/night) or high temperature (38/33 °C day/night) conditions for 35 d via growth chambers at Griffin, GA. Principal component analysis and clustering analysis were performed to rank stress performance and divide lines into different groups according to their tolerance similarities, respectively. At the end of the trial, S11 729-10 and BTC032 were in the most thermotolerant group, followed by a group containing BTC011, AU Victory and Penncross. Crenshaw belonged to the most heat-sensitive group while S11 675-02 and Pure Eclipse were in the second most heat-sensitive group. The exceptional thermotolerance in S11 729-10 and BTC032 was associated with their abilities to maintain cell membrane stability and protein metabolism, plus minimize oxidative damages. Additionally, among various light-harvesting steps, energy trapping, dissipation and electron transport from Q<sub>A</sub> to PQ were more heat-sensitive than electron transport from Q<sub>A</sub> to final PSI acceptors. Along with the strong correlations between multiple OJIP parameters and other traits, it reveals that OJIP fluorescence could be a valuable tool for dissection of photosynthetic processes and identification of the critical steps responsible for photosynthetic declines, enabling a more targeted heat-stress screening. Our results indicated that variability in the level of heat tolerance and associated mechanisms in creeping bentgrass germplasm could be utilized to develop new cultivars with improved thermotolerance.https://www.mdpi.com/2223-7747/12/1/41heat stresscreeping bentgrassOJIP fluorescence |
| spellingShingle | Qianqian Fan David Jespersen Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses Plants heat stress creeping bentgrass OJIP fluorescence |
| title | Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses |
| title_full | Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses |
| title_fullStr | Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses |
| title_full_unstemmed | Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses |
| title_short | Assessing Heat Tolerance in Creeping Bentgrass Lines Based on Physiological Responses |
| title_sort | assessing heat tolerance in creeping bentgrass lines based on physiological responses |
| topic | heat stress creeping bentgrass OJIP fluorescence |
| url | https://www.mdpi.com/2223-7747/12/1/41 |
| work_keys_str_mv | AT qianqianfan assessingheattoleranceincreepingbentgrasslinesbasedonphysiologicalresponses AT davidjespersen assessingheattoleranceincreepingbentgrasslinesbasedonphysiologicalresponses |