| Summary: | Steam explosion pretreatment of lignocellulosic biomass presents a promising technology for agricultural residues before anaerobic degradation. This study aimed to assess biogas production in continuously stirred tank reactors using steam-exploded maize stover mono-digestion. The continuous digestion tests were carried out in four fermenters with a capacity of 150 L under mesophilic and thermophilic conditions. Maize stover was pretreated at 173 °C for 15 min. Four different organic loading rates (OLR) were tested, the biogas and methane production rate was monitored, and parameters such as dry matter (DM), volatile solids (VS), pH, and C:N were analyzed. The results of the tests showed that using steam-exploded maize stover in a continuous system over the range of an OLR from 1.0 to 3.5 kg VS m<sup>–3</sup> d<sup>–1</sup> is feasible with nitrogen as an additive only. The maximum methane yield, 637 L<sub>N</sub> m<sup>–3</sup> d<sup>–1</sup>, was measured under thermophilic conditions with an OLR of 3.5 kg VS m<sup>–3</sup> d<sup>–1</sup>. The trend of an increased gas production rate with an increasing OLR was observed over the range of the applied OLRs, although the average gas yield in the thermophilic mode was higher than it was in the mesophilic one.
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