INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C
Abstract. Detection of circulating autoantibodies provides valuable diagnostic criteria for autoimmune hepatitis (AIH) and primary biliary cirrhosis (PBC). In this study, a panel of autoantibodies (ANA, ASMA, AMA) and ANCA was determined by indirect immunofluorescence assay in 191 patients, includin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/646 |
| _version_ | 1797198011750678528 |
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| author | K. L. Raikhelson U. A. Mitelglik A. S. Zubareva N. V. Dunaeva T. V. Bulgakova S. V. Lapin A. Yu. Baranovsky A. Totolian Areg |
| author_facet | K. L. Raikhelson U. A. Mitelglik A. S. Zubareva N. V. Dunaeva T. V. Bulgakova S. V. Lapin A. Yu. Baranovsky A. Totolian Areg |
| author_sort | K. L. Raikhelson |
| collection | DOAJ |
| description | Abstract. Detection of circulating autoantibodies provides valuable diagnostic criteria for autoimmune hepatitis (AIH) and primary biliary cirrhosis (PBC). In this study, a panel of autoantibodies (ANA, ASMA, AMA) and ANCA was determined by indirect immunofluorescence assay in 191 patients, including those with AIH type 1 (n = 42), with PBC (n = 39), with overlapping AIH-1/PBC syndrome (n = 20), and 100 patients with viral hepatitis С (HCV). Autoantibodies against PDC/AMA-M2, M2-3E, PML, Sp100, gp210, SSA-Ro52, SLA/LP, LC-1 were detected by means of immune blotting in 53 patients with autoimmune liver diseases (AIH, 19; PBC, 23; AIH/PBC, 11). ANA were detected in 88.1% of AIH-1 patients, 89.7% of PBC cases, and 100% of combined AIH-1/PBC syndrome, with highest ANA titers observed in the latter group. In patients with HCV, ANA were detected in 20%, and ASMA in 2% of the cases.ASMA and ANCA were found in monovariant AIH only (61.9% for ASMA, р < 0.001), and 35.7% for ANCA (р < 0.01). Anti-SLA/LP were detected in 15% of AIH patients, mostly with negative ANA and ASMA. PDC/AMA-M2 were identified in all patients with PBC and AIH-1/PBC, whereas AMA was detectable in 84.6% of PBC patients. Anti-M2-3E antibodies were found only in patients with PBC including those with an overlap syndrome. Occurence of detectable antibodies to gp210, Sp100, PML and SSA-Ro52 was similar in all groups. In our study, we have not confirmed a view that anti-gp210 and anti-Sp100 antibodies are highly specific for primary biliary cirrhosis. |
| first_indexed | 2024-03-08T05:49:36Z |
| format | Article |
| id | doaj.art-56b3e3175d4c41ebb8a7c84775b38c51 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:53:05Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-56b3e3175d4c41ebb8a7c84775b38c512024-04-22T13:07:38ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0115435136010.15789/1563-0625-2013-4-351-360643INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS CK. L. Raikhelson0U. A. Mitelglik1A. S. Zubareva2N. V. Dunaeva3T. V. Bulgakova4S. V. Lapin5A. Yu. Baranovsky6A. Totolian Areg7North-Western I. Mechnikov State Medical University, St. PetersburgNorth-Western I. Mechnikov State Medical University, St. PetersburgSt. Petersburg Municipal Clinical Hospital No. 31, St. PetersburgResearch Institute of Influenza, St. PetersburgSt. Petersburg I.P. Pavlov State Medical University, St. PetersburgSt. Petersburg I.P. Pavlov State Medical University, St. PetersburgNorth-Western I. Mechnikov State Medical University, St. PetersburgPasteur Institute of Epidemiology and Microbiology, St. PetersburgAbstract. Detection of circulating autoantibodies provides valuable diagnostic criteria for autoimmune hepatitis (AIH) and primary biliary cirrhosis (PBC). In this study, a panel of autoantibodies (ANA, ASMA, AMA) and ANCA was determined by indirect immunofluorescence assay in 191 patients, including those with AIH type 1 (n = 42), with PBC (n = 39), with overlapping AIH-1/PBC syndrome (n = 20), and 100 patients with viral hepatitis С (HCV). Autoantibodies against PDC/AMA-M2, M2-3E, PML, Sp100, gp210, SSA-Ro52, SLA/LP, LC-1 were detected by means of immune blotting in 53 patients with autoimmune liver diseases (AIH, 19; PBC, 23; AIH/PBC, 11). ANA were detected in 88.1% of AIH-1 patients, 89.7% of PBC cases, and 100% of combined AIH-1/PBC syndrome, with highest ANA titers observed in the latter group. In patients with HCV, ANA were detected in 20%, and ASMA in 2% of the cases.ASMA and ANCA were found in monovariant AIH only (61.9% for ASMA, р < 0.001), and 35.7% for ANCA (р < 0.01). Anti-SLA/LP were detected in 15% of AIH patients, mostly with negative ANA and ASMA. PDC/AMA-M2 were identified in all patients with PBC and AIH-1/PBC, whereas AMA was detectable in 84.6% of PBC patients. Anti-M2-3E antibodies were found only in patients with PBC including those with an overlap syndrome. Occurence of detectable antibodies to gp210, Sp100, PML and SSA-Ro52 was similar in all groups. In our study, we have not confirmed a view that anti-gp210 and anti-Sp100 antibodies are highly specific for primary biliary cirrhosis.https://www.mimmun.ru/mimmun/article/view/646laboratory diagnosticsautoantibodiesautoimmune hepatitisprimary biliary cirrhosischronic hepatitis c |
| spellingShingle | K. L. Raikhelson U. A. Mitelglik A. S. Zubareva N. V. Dunaeva T. V. Bulgakova S. V. Lapin A. Yu. Baranovsky A. Totolian Areg INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C Медицинская иммунология laboratory diagnostics autoantibodies autoimmune hepatitis primary biliary cirrhosis chronic hepatitis c |
| title | INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C |
| title_full | INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C |
| title_fullStr | INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C |
| title_full_unstemmed | INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C |
| title_short | INCIDENCE OF AUTOANTIBODIES IN PATIENTS WITH AUTOIMMUNE LIVER DISEASES AND CHRONIC HEPATITIS C |
| title_sort | incidence of autoantibodies in patients with autoimmune liver diseases and chronic hepatitis c |
| topic | laboratory diagnostics autoantibodies autoimmune hepatitis primary biliary cirrhosis chronic hepatitis c |
| url | https://www.mimmun.ru/mimmun/article/view/646 |
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