A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus.
Staphylococcus aureus colonies can spread on soft agar plates. We compared colony spreading of clinically isolated methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). All MSSA strains showed colony spreading, but most MRSA strains (73%) carrying SCCmec type-II showed l...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2593785?pdf=render |
| _version_ | 1811274509364232192 |
|---|---|
| author | Chikara Kaito Yosuke Omae Yasuhiko Matsumoto Makiko Nagata Hiroki Yamaguchi Taiji Aoto Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu |
| author_facet | Chikara Kaito Yosuke Omae Yasuhiko Matsumoto Makiko Nagata Hiroki Yamaguchi Taiji Aoto Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu |
| author_sort | Chikara Kaito |
| collection | DOAJ |
| description | Staphylococcus aureus colonies can spread on soft agar plates. We compared colony spreading of clinically isolated methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). All MSSA strains showed colony spreading, but most MRSA strains (73%) carrying SCCmec type-II showed little colony spreading. Deletion of the entire SCCmec type-II region from these MRSA strains restored colony spreading. Introduction of a novel gene, fudoh, carried by SCCmec type-II into Newman strain suppressed colony spreading. MRSA strains with high spreading ability (27%) had no fudoh or a point-mutated fudoh that did not suppress colony spreading. The fudoh-transformed Newman strain had decreased exotoxin production and attenuated virulence in mice. Most community-acquired MRSA strains carried SCCmec type-IV, which does not include fudoh, and showed high colony spreading ability. These findings suggest that fudoh in the SCCmec type-II region suppresses colony spreading and exotoxin production, and is involved in S. aureus pathogenesis. |
| first_indexed | 2024-04-12T23:20:27Z |
| format | Article |
| id | doaj.art-56b4dde55b8c4cf3a67d4e028c510dc7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T23:20:27Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-56b4dde55b8c4cf3a67d4e028c510dc72022-12-22T03:12:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01312e392110.1371/journal.pone.0003921A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus.Chikara KaitoYosuke OmaeYasuhiko MatsumotoMakiko NagataHiroki YamaguchiTaiji AotoTeruyo ItoKeiichi HiramatsuKazuhisa SekimizuStaphylococcus aureus colonies can spread on soft agar plates. We compared colony spreading of clinically isolated methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). All MSSA strains showed colony spreading, but most MRSA strains (73%) carrying SCCmec type-II showed little colony spreading. Deletion of the entire SCCmec type-II region from these MRSA strains restored colony spreading. Introduction of a novel gene, fudoh, carried by SCCmec type-II into Newman strain suppressed colony spreading. MRSA strains with high spreading ability (27%) had no fudoh or a point-mutated fudoh that did not suppress colony spreading. The fudoh-transformed Newman strain had decreased exotoxin production and attenuated virulence in mice. Most community-acquired MRSA strains carried SCCmec type-IV, which does not include fudoh, and showed high colony spreading ability. These findings suggest that fudoh in the SCCmec type-II region suppresses colony spreading and exotoxin production, and is involved in S. aureus pathogenesis.http://europepmc.org/articles/PMC2593785?pdf=render |
| spellingShingle | Chikara Kaito Yosuke Omae Yasuhiko Matsumoto Makiko Nagata Hiroki Yamaguchi Taiji Aoto Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. PLoS ONE |
| title | A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. |
| title_full | A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. |
| title_fullStr | A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. |
| title_full_unstemmed | A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. |
| title_short | A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. |
| title_sort | novel gene fudoh in the sccmec region suppresses the colony spreading ability and virulence of staphylococcus aureus |
| url | http://europepmc.org/articles/PMC2593785?pdf=render |
| work_keys_str_mv | AT chikarakaito anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT yosukeomae anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT yasuhikomatsumoto anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT makikonagata anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT hirokiyamaguchi anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT taijiaoto anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT teruyoito anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT keiichihiramatsu anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT kazuhisasekimizu anovelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT chikarakaito novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT yosukeomae novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT yasuhikomatsumoto novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT makikonagata novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT hirokiyamaguchi novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT taijiaoto novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT teruyoito novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT keiichihiramatsu novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus AT kazuhisasekimizu novelgenefudohinthesccmecregionsuppressesthecolonyspreadingabilityandvirulenceofstaphylococcusaureus |