PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments
Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2015-03-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/293 |
| _version_ | 1828027161185878016 |
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| author | Homaira Anzum Himi Md. Shafiullah Parvej M. Bahanur Rahman K M Nasiruddin Wahedul Karim Ansari Md. Mostakin Ahamed |
| author_facet | Homaira Anzum Himi Md. Shafiullah Parvej M. Bahanur Rahman K M Nasiruddin Wahedul Karim Ansari Md. Mostakin Ahamed |
| author_sort | Homaira Anzum Himi |
| collection | DOAJ |
| description | Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. coli in poultry, detection of its source of infection in poultry and transmission pattern to human. For this purpose a total of 150 samples (cloacal swab-60, feed -15, water-15 and egg -60) were collected and analyzed in bacteriology laboratory by cultured in different bacteriological media followed by gram’s staining, biochemical tests and Polymerase Chain reaction (PCR). The PCR was performed by targeting 16s rRNA gene and shiga toxin producing gene in E. coli. Out of 150 collected samples, E. coli was found in 81 (54%) samples. Presence of E. coli was 100% in both feed (n=15) and egg (n=60), whereas 10% in cloacal swab (n=6). Water samples were totally free of E. coli. The stx2 gene was detected in all samples whether all samples were negative for stx1 gene. The study revealed that, poultry feed acts as a source of E. coli infection in poultry, which may be transmitted to environment and human via meat or eggs. Antibiotic sensitivity test revealed that isolated bacteria were highly sensitive to Ciprofloxacin. |
| first_indexed | 2024-04-10T13:29:56Z |
| format | Article |
| id | doaj.art-56b6172691f04ce986ab9425bdf38ab9 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T13:29:56Z |
| publishDate | 2015-03-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-56b6172691f04ce986ab9425bdf38ab92023-02-15T16:11:39ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2015-03-0136145PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related EnvironmentsHomaira Anzum Himi0Md. Shafiullah Parvej1M. Bahanur RahmanK M Nasiruddin2Wahedul Karim Ansari3Md. Mostakin Ahamed4Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202,Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. coli in poultry, detection of its source of infection in poultry and transmission pattern to human. For this purpose a total of 150 samples (cloacal swab-60, feed -15, water-15 and egg -60) were collected and analyzed in bacteriology laboratory by cultured in different bacteriological media followed by gram’s staining, biochemical tests and Polymerase Chain reaction (PCR). The PCR was performed by targeting 16s rRNA gene and shiga toxin producing gene in E. coli. Out of 150 collected samples, E. coli was found in 81 (54%) samples. Presence of E. coli was 100% in both feed (n=15) and egg (n=60), whereas 10% in cloacal swab (n=6). Water samples were totally free of E. coli. The stx2 gene was detected in all samples whether all samples were negative for stx1 gene. The study revealed that, poultry feed acts as a source of E. coli infection in poultry, which may be transmitted to environment and human via meat or eggs. Antibiotic sensitivity test revealed that isolated bacteria were highly sensitive to Ciprofloxacin.http://www.agrifoodscience.com/index.php/TURJAF/article/view/293Commercial PoultryCiprofloxacinE. coliShiga toxinPCR |
| spellingShingle | Homaira Anzum Himi Md. Shafiullah Parvej M. Bahanur Rahman K M Nasiruddin Wahedul Karim Ansari Md. Mostakin Ahamed PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments Turkish Journal of Agriculture: Food Science and Technology Commercial Poultry Ciprofloxacin E. coli Shiga toxin PCR |
| title | PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments |
| title_full | PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments |
| title_fullStr | PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments |
| title_full_unstemmed | PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments |
| title_short | PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments |
| title_sort | pcr based detection of shiga toxin producing e coli in commercial poultry and related environments |
| topic | Commercial Poultry Ciprofloxacin E. coli Shiga toxin PCR |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/293 |
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