Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey
Abstract Background The emergence of tick-borne disease is increasing because of the effects of the temperature rise driven by global warming. In Turkey, 19 pathogens transmitted by ticks to humans and animals have been reported. Based on this, this study aimed to investigate tick-borne pathogens in...
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-021-04779-2 |
| _version_ | 1819095857152131072 |
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| author | Ahmet Efe Köseoğlu Hüseyin Can Mervenur Güvendi Sedef Erkunt Alak Çağrı Kandemir Turğay Taşkın Samiye Demir Gülşah Akgül Aysu Değirmenci Döşkaya Muhammet Karakavuk Mert Döşkaya Adnan Yüksel Gürüz Cemal Ün |
| author_facet | Ahmet Efe Köseoğlu Hüseyin Can Mervenur Güvendi Sedef Erkunt Alak Çağrı Kandemir Turğay Taşkın Samiye Demir Gülşah Akgül Aysu Değirmenci Döşkaya Muhammet Karakavuk Mert Döşkaya Adnan Yüksel Gürüz Cemal Ün |
| author_sort | Ahmet Efe Köseoğlu |
| collection | DOAJ |
| description | Abstract Background The emergence of tick-borne disease is increasing because of the effects of the temperature rise driven by global warming. In Turkey, 19 pathogens transmitted by ticks to humans and animals have been reported. Based on this, this study aimed to investigate tick-borne pathogens including Hepatozoon spp., Theileria spp., Babesia spp., Anaplasma spp., Borrelia spp., and Bartonella spp. in tick samples (n = 110) collected from different hosts (dogs, cats, cattle, goats, sheep, and turtles) by molecular methods. Methods To meet this objective, ticks were identified morphologically at the genus level by microscopy; after DNA isolation, each tick sample was identified at the species level using the molecular method. Involved pathogens were then investigated by PCR method. Results Seven different tick species were identified including Rhipicephalus sanguineus, R. turanicus, R. bursa, Hyalomma marginatum, H. anatolicum, H. aegyptium, and Haemaphysalis erinacei. Among the analyzed ticks, Hepatozoon spp., Theileria spp., Babesia spp., and Anaplasma spp. were detected at rates of 6.36%, 16.3%, 1.81%, and 6.36%, respectively while Borrelia spp. and Bartonella spp. were not detected. Hepatozoon spp. was detected in R. sanguineus ticks while Theileria spp., Babesia spp., and Anaplasma spp. were detected in R. turanicus and H. marginatum. According to the results of sequence analyses applied for pathogen positive samples, Hepatozoon canis, Theileria ovis, Babesia caballi, and Anaplasma ovis were identified. Conclusion Theileria ovis and Anaplasma ovis were detected for the first time to our knowledge in H. marginatum and R. turanicus collected from Turkey, respectively. Also, B. caballi was detected for the first time to our knowledge in ticks in Turkey. |
| first_indexed | 2024-12-21T23:49:58Z |
| format | Article |
| id | doaj.art-56b7c69c700942dc92475205d199bebe |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-21T23:49:58Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-56b7c69c700942dc92475205d199bebe2022-12-21T18:45:58ZengBMCParasites & Vectors1756-33052021-05-0114111110.1186/s13071-021-04779-2Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in TurkeyAhmet Efe Köseoğlu0Hüseyin Can1Mervenur Güvendi2Sedef Erkunt Alak3Çağrı Kandemir4Turğay Taşkın5Samiye Demir6Gülşah Akgül7Aysu Değirmenci Döşkaya8Muhammet Karakavuk9Mert Döşkaya10Adnan Yüksel Gürüz11Cemal Ün12Molecular Biology Section, Department of Biology, Faculty of Science, Ege UniversityMolecular Biology Section, Department of Biology, Faculty of Science, Ege UniversityMolecular Biology Section, Department of Biology, Faculty of Science, Ege UniversityMolecular Biology Section, Department of Biology, Faculty of Science, Ege UniversityDepartment of Animal Science, Faculty of Agriculture, Ege UniversityDepartment of Animal Science, Faculty of Agriculture, Ege UniversityZoology Section, Department of Biology, Faculty of Science, Ege UniversityDepartment of Internal Medicine, Faculty of Veterinary Medicine, Siirt UniversityDepartment of Parasitology, Faculty of Medicine, Ege UniversityÖdemiş Technical Training College, Ege UniversityDepartment of Parasitology, Faculty of Medicine, Ege UniversityDepartment of Parasitology, Faculty of Medicine, Ege UniversityMolecular Biology Section, Department of Biology, Faculty of Science, Ege UniversityAbstract Background The emergence of tick-borne disease is increasing because of the effects of the temperature rise driven by global warming. In Turkey, 19 pathogens transmitted by ticks to humans and animals have been reported. Based on this, this study aimed to investigate tick-borne pathogens including Hepatozoon spp., Theileria spp., Babesia spp., Anaplasma spp., Borrelia spp., and Bartonella spp. in tick samples (n = 110) collected from different hosts (dogs, cats, cattle, goats, sheep, and turtles) by molecular methods. Methods To meet this objective, ticks were identified morphologically at the genus level by microscopy; after DNA isolation, each tick sample was identified at the species level using the molecular method. Involved pathogens were then investigated by PCR method. Results Seven different tick species were identified including Rhipicephalus sanguineus, R. turanicus, R. bursa, Hyalomma marginatum, H. anatolicum, H. aegyptium, and Haemaphysalis erinacei. Among the analyzed ticks, Hepatozoon spp., Theileria spp., Babesia spp., and Anaplasma spp. were detected at rates of 6.36%, 16.3%, 1.81%, and 6.36%, respectively while Borrelia spp. and Bartonella spp. were not detected. Hepatozoon spp. was detected in R. sanguineus ticks while Theileria spp., Babesia spp., and Anaplasma spp. were detected in R. turanicus and H. marginatum. According to the results of sequence analyses applied for pathogen positive samples, Hepatozoon canis, Theileria ovis, Babesia caballi, and Anaplasma ovis were identified. Conclusion Theileria ovis and Anaplasma ovis were detected for the first time to our knowledge in H. marginatum and R. turanicus collected from Turkey, respectively. Also, B. caballi was detected for the first time to our knowledge in ticks in Turkey.https://doi.org/10.1186/s13071-021-04779-2Hepatozoon canisTheileria ovisBabesia caballiAnaplasma ovis |
| spellingShingle | Ahmet Efe Köseoğlu Hüseyin Can Mervenur Güvendi Sedef Erkunt Alak Çağrı Kandemir Turğay Taşkın Samiye Demir Gülşah Akgül Aysu Değirmenci Döşkaya Muhammet Karakavuk Mert Döşkaya Adnan Yüksel Gürüz Cemal Ün Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey Parasites & Vectors Hepatozoon canis Theileria ovis Babesia caballi Anaplasma ovis |
| title | Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey |
| title_full | Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey |
| title_fullStr | Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey |
| title_full_unstemmed | Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey |
| title_short | Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey |
| title_sort | molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in turkey |
| topic | Hepatozoon canis Theileria ovis Babesia caballi Anaplasma ovis |
| url | https://doi.org/10.1186/s13071-021-04779-2 |
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