Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface.
To reveal the effect of p53-tristetraprolin-stathmin-1 signaling on trophoblasts and recurrent spontaneous abortion (RSA).Stathmin-1 (STMN1), p53, and tristetraprolin (TTP) expression in paraffin-embedded villus tissue was determined using immunohistochemistry. HTR-8/SVneo cells were treated with do...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5489185?pdf=render |
| _version_ | 1818030003716096000 |
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| author | Xiao-Ling Ma Xiao-Cui Li Fu-Ju Tian Si-Ming Zhang Xiao-Rui Liu Yan Zhang Jian-Xia Fan Yi Lin |
| author_facet | Xiao-Ling Ma Xiao-Cui Li Fu-Ju Tian Si-Ming Zhang Xiao-Rui Liu Yan Zhang Jian-Xia Fan Yi Lin |
| author_sort | Xiao-Ling Ma |
| collection | DOAJ |
| description | To reveal the effect of p53-tristetraprolin-stathmin-1 signaling on trophoblasts and recurrent spontaneous abortion (RSA).Stathmin-1 (STMN1), p53, and tristetraprolin (TTP) expression in paraffin-embedded villus tissue was determined using immunohistochemistry. HTR-8/SVneo cells were treated with doxorubicin to activate p53; STMN1 and TTP levels were detected by quantitative reverse transcription-PCR and western blotting. Western blotting and immunofluorescence were used to investigate STMN1 expression after TTP overexpression or knockdown in HTR-8 cells.STMN1 was downregulated and p53 was upregulated in the villus tissue from patients with RSA. Doxorubicin decreased STMN1 expression but enhanced TTP expression in HTR-8 cells. In vitro, TTP overexpression inhibited STMN1 production; TTP knockdown promoted it. TTP downregulated STMN1 expression in trophoblasts by directly binding its 3' untranslated region.TTP modulates trophoblast function and interacts with STMN1 and p53, and is related to pregnancy outcomes. |
| first_indexed | 2024-12-10T05:28:40Z |
| format | Article |
| id | doaj.art-56b857cf204e4563a3b2a63c04b704e9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:28:40Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-56b857cf204e4563a3b2a63c04b704e92022-12-22T02:00:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017985210.1371/journal.pone.0179852Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface.Xiao-Ling MaXiao-Cui LiFu-Ju TianSi-Ming ZhangXiao-Rui LiuYan ZhangJian-Xia FanYi LinTo reveal the effect of p53-tristetraprolin-stathmin-1 signaling on trophoblasts and recurrent spontaneous abortion (RSA).Stathmin-1 (STMN1), p53, and tristetraprolin (TTP) expression in paraffin-embedded villus tissue was determined using immunohistochemistry. HTR-8/SVneo cells were treated with doxorubicin to activate p53; STMN1 and TTP levels were detected by quantitative reverse transcription-PCR and western blotting. Western blotting and immunofluorescence were used to investigate STMN1 expression after TTP overexpression or knockdown in HTR-8 cells.STMN1 was downregulated and p53 was upregulated in the villus tissue from patients with RSA. Doxorubicin decreased STMN1 expression but enhanced TTP expression in HTR-8 cells. In vitro, TTP overexpression inhibited STMN1 production; TTP knockdown promoted it. TTP downregulated STMN1 expression in trophoblasts by directly binding its 3' untranslated region.TTP modulates trophoblast function and interacts with STMN1 and p53, and is related to pregnancy outcomes.http://europepmc.org/articles/PMC5489185?pdf=render |
| spellingShingle | Xiao-Ling Ma Xiao-Cui Li Fu-Ju Tian Si-Ming Zhang Xiao-Rui Liu Yan Zhang Jian-Xia Fan Yi Lin Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface. PLoS ONE |
| title | Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface. |
| title_full | Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface. |
| title_fullStr | Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface. |
| title_full_unstemmed | Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface. |
| title_short | Effect of the p53-tristetraprolin-stathmin-1 pathway on trophoblasts at maternal-fetal interface. |
| title_sort | effect of the p53 tristetraprolin stathmin 1 pathway on trophoblasts at maternal fetal interface |
| url | http://europepmc.org/articles/PMC5489185?pdf=render |
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