Enhancement of homology-directed repair with chromatin donor templates in cells
A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of D...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/55780 |
| _version_ | 1811235971682795520 |
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| author | Grisel Cruz-Becerra James T Kadonaga |
| author_facet | Grisel Cruz-Becerra James T Kadonaga |
| author_sort | Grisel Cruz-Becerra |
| collection | DOAJ |
| description | A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, increases the frequency of HDR-edited clones as well as homozygous editing. In addition, transfection of chromatin results in negligible cytotoxicity. These findings suggest that a chromatin donor template should be useful for a wide range of HDR applications such as the precise insertion or replacement of DNA fragments that contain the coding regions of genes. |
| first_indexed | 2024-04-12T12:01:15Z |
| format | Article |
| id | doaj.art-56bde30bb8204a57b3f06b568859a28d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:01:15Z |
| publishDate | 2020-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-56bde30bb8204a57b3f06b568859a28d2022-12-22T03:33:50ZengeLife Sciences Publications LtdeLife2050-084X2020-04-01910.7554/eLife.55780Enhancement of homology-directed repair with chromatin donor templates in cellsGrisel Cruz-Becerra0https://orcid.org/0000-0001-6297-4132James T Kadonaga1https://orcid.org/0000-0002-2075-9458Section of Molecular Biology, University of California, San Diego, La Jolla, United StatesSection of Molecular Biology, University of California, San Diego, La Jolla, United StatesA key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, increases the frequency of HDR-edited clones as well as homozygous editing. In addition, transfection of chromatin results in negligible cytotoxicity. These findings suggest that a chromatin donor template should be useful for a wide range of HDR applications such as the precise insertion or replacement of DNA fragments that contain the coding regions of genes.https://elifesciences.org/articles/55780chromatinhomology-directed repairprecise genome editing |
| spellingShingle | Grisel Cruz-Becerra James T Kadonaga Enhancement of homology-directed repair with chromatin donor templates in cells eLife chromatin homology-directed repair precise genome editing |
| title | Enhancement of homology-directed repair with chromatin donor templates in cells |
| title_full | Enhancement of homology-directed repair with chromatin donor templates in cells |
| title_fullStr | Enhancement of homology-directed repair with chromatin donor templates in cells |
| title_full_unstemmed | Enhancement of homology-directed repair with chromatin donor templates in cells |
| title_short | Enhancement of homology-directed repair with chromatin donor templates in cells |
| title_sort | enhancement of homology directed repair with chromatin donor templates in cells |
| topic | chromatin homology-directed repair precise genome editing |
| url | https://elifesciences.org/articles/55780 |
| work_keys_str_mv | AT griselcruzbecerra enhancementofhomologydirectedrepairwithchromatindonortemplatesincells AT jamestkadonaga enhancementofhomologydirectedrepairwithchromatindonortemplatesincells |