A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide
Recurrent aphthous ulcer (RAU), one of the most common diseases in humans, has an unknown etiology and is difficult to treat. Thalidomide is an important immunomodulatory and antitumor drug and its effects on the gut microbiota still remain unclear. We conducted a metagenomic sequencing study of fec...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1018567/full |
| _version_ | 1811237605328551936 |
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| author | Xiang Wang Kexu Xiong Fan Huang Jinqun Huang Qin Liu Ning Duan Huanhuan Ruan Hongliu Jiang Yanan Zhu Lin Lin Yuefeng Song Maomao Zhao Lichun Zheng Pei Ye Yajie Qian Qingang Hu Fuhua Yan Wenmei Wang |
| author_facet | Xiang Wang Kexu Xiong Fan Huang Jinqun Huang Qin Liu Ning Duan Huanhuan Ruan Hongliu Jiang Yanan Zhu Lin Lin Yuefeng Song Maomao Zhao Lichun Zheng Pei Ye Yajie Qian Qingang Hu Fuhua Yan Wenmei Wang |
| author_sort | Xiang Wang |
| collection | DOAJ |
| description | Recurrent aphthous ulcer (RAU), one of the most common diseases in humans, has an unknown etiology and is difficult to treat. Thalidomide is an important immunomodulatory and antitumor drug and its effects on the gut microbiota still remain unclear. We conducted a metagenomic sequencing study of fecal samples from a cohort of individuals with RAU, performed biochemical assays of cytokines, immunoglobulins and antimicrobial peptides in serum and saliva, and investigated the regulation effects of thalidomide administration and withdrawal. Meanwhile we constructed the corresponding prediction models. Our metagenome-wide association results indicated that gut dysbacteriosis, microbial dysfunction and immune imbalance occurred in RAU patients. Thalidomide regulated gut dysbacteriosis in a species-specific manner and had different sustainable effects on various probiotics and pathogens. A previously unknown association between gut microbiota alterations and RAU was found, and the specific roles of thalidomide in modulating the gut microbiota and immunity were determined, suggesting that RAU may be affected by targeting gut dysbacteriosis and modifying immune imbalance. In-depth insights into sophisticated networks consisting of the gut microbiota and host cells may lead to the development of emerging treatments, including prebiotics, probiotics, synbiotics, and postbiotics. |
| first_indexed | 2024-04-12T12:27:40Z |
| format | Article |
| id | doaj.art-56c14b2af835403dbc86a425679188b8 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T12:27:40Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-56c14b2af835403dbc86a425679188b82022-12-22T03:33:08ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-10-011310.3389/fimmu.2022.10185671018567A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomideXiang Wang0Kexu Xiong1Fan Huang2Jinqun Huang3Qin Liu4Ning Duan5Huanhuan Ruan6Hongliu Jiang7Yanan Zhu8Lin Lin9Yuefeng Song10Maomao Zhao11Lichun Zheng12Pei Ye13Yajie Qian14Qingang Hu15Fuhua Yan16Wenmei Wang17Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaBeijing Genomics Institute (BGI)-genomics, BGI-Shenzhen, Shenzhen, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaNanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaRecurrent aphthous ulcer (RAU), one of the most common diseases in humans, has an unknown etiology and is difficult to treat. Thalidomide is an important immunomodulatory and antitumor drug and its effects on the gut microbiota still remain unclear. We conducted a metagenomic sequencing study of fecal samples from a cohort of individuals with RAU, performed biochemical assays of cytokines, immunoglobulins and antimicrobial peptides in serum and saliva, and investigated the regulation effects of thalidomide administration and withdrawal. Meanwhile we constructed the corresponding prediction models. Our metagenome-wide association results indicated that gut dysbacteriosis, microbial dysfunction and immune imbalance occurred in RAU patients. Thalidomide regulated gut dysbacteriosis in a species-specific manner and had different sustainable effects on various probiotics and pathogens. A previously unknown association between gut microbiota alterations and RAU was found, and the specific roles of thalidomide in modulating the gut microbiota and immunity were determined, suggesting that RAU may be affected by targeting gut dysbacteriosis and modifying immune imbalance. In-depth insights into sophisticated networks consisting of the gut microbiota and host cells may lead to the development of emerging treatments, including prebiotics, probiotics, synbiotics, and postbiotics.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1018567/fullgut microbiotarecurrent aphthous ulcermetagenomeimmunitythalidomide |
| spellingShingle | Xiang Wang Kexu Xiong Fan Huang Jinqun Huang Qin Liu Ning Duan Huanhuan Ruan Hongliu Jiang Yanan Zhu Lin Lin Yuefeng Song Maomao Zhao Lichun Zheng Pei Ye Yajie Qian Qingang Hu Fuhua Yan Wenmei Wang A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide Frontiers in Immunology gut microbiota recurrent aphthous ulcer metagenome immunity thalidomide |
| title | A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide |
| title_full | A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide |
| title_fullStr | A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide |
| title_full_unstemmed | A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide |
| title_short | A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide |
| title_sort | metagenome wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide |
| topic | gut microbiota recurrent aphthous ulcer metagenome immunity thalidomide |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1018567/full |
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