Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa
OBJECTIVE: To evaluate the performance of serological tests using dried blood on filter-papers (micro-card agglutination test for trypanosomiasis (micro-CATT)) performed under field and laboratory conditions and using whole blood ((CATT/T.b. gambiense) (wb-CATT) and latex agglutination (LATEX/T.b. g...
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| Format: | Article |
| Language: | English |
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The World Health Organization
2002-01-01
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| Series: | Bulletin of the World Health Organization |
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| Online Access: | http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001100008 |
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| author | Truc Philippe Lejon Veerle Magnus Eddy Jamonneau Vincent Nangouma Auguste Verloo Didier Penchenier Laurent Büscher Philippe |
| author_facet | Truc Philippe Lejon Veerle Magnus Eddy Jamonneau Vincent Nangouma Auguste Verloo Didier Penchenier Laurent Büscher Philippe |
| author_sort | Truc Philippe |
| collection | DOAJ |
| description | OBJECTIVE: To evaluate the performance of serological tests using dried blood on filter-papers (micro-card agglutination test for trypanosomiasis (micro-CATT)) performed under field and laboratory conditions and using whole blood ((CATT/T.b. gambiense) (wb-CATT) and latex agglutination (LATEX/T.b. gambiense) (wb-LATEX)) for the serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa. METHODS: We evaluated the micro-CATT, wb-CATT and wb-LATEX methods in Côte d'Ivoire and the Central African Republic by screening 940 people. Sensitivity and specificity were calculated for each serological test; only patients with the confirmed presence of trypanosomes in the blood or lymph aspirate were considered true positives. Positive and negative predictive values were also calculated. FINDINGS: Each of the tests showed a lower sensitivity in the Central African Republic than in Côte d'Ivoire. CONCLUSION: The results confirmed the efficiency of the classic wb-CATT to detect sleeping sickness patients. The micro-CATT method can be used for human African trypanosomiasis surveillance if the test is performed on the same day as the blood collection, or if samples are stored at 4 ºC. Otherwise, micro-CATT can be used when absolute sensitivity is not required. wb-LATEX should only be used for high-specificity screening. |
| first_indexed | 2024-03-07T18:34:00Z |
| format | Article |
| id | doaj.art-56c36e353ed54d1695335cb93fb1d41c |
| institution | Directory Open Access Journal |
| issn | 0042-9686 |
| language | English |
| last_indexed | 2024-03-07T18:34:00Z |
| publishDate | 2002-01-01 |
| publisher | The World Health Organization |
| record_format | Article |
| series | Bulletin of the World Health Organization |
| spelling | doaj.art-56c36e353ed54d1695335cb93fb1d41c2024-03-02T05:06:25ZengThe World Health OrganizationBulletin of the World Health Organization0042-96862002-01-018011882886Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central AfricaTruc PhilippeLejon VeerleMagnus EddyJamonneau VincentNangouma AugusteVerloo DidierPenchenier LaurentBüscher PhilippeOBJECTIVE: To evaluate the performance of serological tests using dried blood on filter-papers (micro-card agglutination test for trypanosomiasis (micro-CATT)) performed under field and laboratory conditions and using whole blood ((CATT/T.b. gambiense) (wb-CATT) and latex agglutination (LATEX/T.b. gambiense) (wb-LATEX)) for the serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa. METHODS: We evaluated the micro-CATT, wb-CATT and wb-LATEX methods in Côte d'Ivoire and the Central African Republic by screening 940 people. Sensitivity and specificity were calculated for each serological test; only patients with the confirmed presence of trypanosomes in the blood or lymph aspirate were considered true positives. Positive and negative predictive values were also calculated. FINDINGS: Each of the tests showed a lower sensitivity in the Central African Republic than in Côte d'Ivoire. CONCLUSION: The results confirmed the efficiency of the classic wb-CATT to detect sleeping sickness patients. The micro-CATT method can be used for human African trypanosomiasis surveillance if the test is performed on the same day as the blood collection, or if samples are stored at 4 ºC. Otherwise, micro-CATT can be used when absolute sensitivity is not required. wb-LATEX should only be used for high-specificity screening.http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001100008Agglutination testsSensitivity and specificityPredictive value of testsTrypanosomiasisAfrican/diagnosisComparative studyCôte d'IvoireCentral African Republic |
| spellingShingle | Truc Philippe Lejon Veerle Magnus Eddy Jamonneau Vincent Nangouma Auguste Verloo Didier Penchenier Laurent Büscher Philippe Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa Bulletin of the World Health Organization Agglutination tests Sensitivity and specificity Predictive value of tests Trypanosomiasis African/diagnosis Comparative study Côte d'Ivoire Central African Republic |
| title | Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa |
| title_full | Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa |
| title_fullStr | Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa |
| title_full_unstemmed | Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa |
| title_short | Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T. b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa |
| title_sort | evaluation of the micro catt catt trypanosoma brucei gambiense and latex t b gambiense methods for serodiagnosis and surveillance of human african trypanosomiasis in west and central africa |
| topic | Agglutination tests Sensitivity and specificity Predictive value of tests Trypanosomiasis African/diagnosis Comparative study Côte d'Ivoire Central African Republic |
| url | http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001100008 |
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