ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
T cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis and sorting of antigen-specific...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-07-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00218/full |
| _version_ | 1828527074577481728 |
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| author | Julien eSchmidt Danijel eDojcinovic Philippe eGuillaume Immanuel eLuescher |
| author_facet | Julien eSchmidt Danijel eDojcinovic Philippe eGuillaume Immanuel eLuescher |
| author_sort | Julien eSchmidt |
| collection | DOAJ |
| description | T cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis and sorting of antigen-specific T cells. This is possible by means of soluble recombinant ligands for T cells i.e. MHC class I-peptide (pMHC I) complexes for CD8+ T cells and MHC class II-peptide (pMHC II) complexes for CD4+ T cells and flow cytometry. Here we review major developments in the development of pMHC staining reagents and their diverse applications and discuss perspectives of their use for basic and clinical investigations. |
| first_indexed | 2024-12-11T21:33:56Z |
| format | Article |
| id | doaj.art-56c4f850c1e542369e70c7e3e5c00c4d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T21:33:56Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-56c4f850c1e542369e70c7e3e5c00c4d2022-12-22T00:50:04ZengFrontiers Media S.A.Frontiers in Immunology1664-32242013-07-01410.3389/fimmu.2013.0021857410ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXESJulien eSchmidt0Danijel eDojcinovic1Philippe eGuillaume2Immanuel eLuescher3Ludwig center for cancer resreach@University of LausanneTCMetrix sàrlTCMetrix sàrlLudwig center for cancer resreach@University of LausanneT cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis and sorting of antigen-specific T cells. This is possible by means of soluble recombinant ligands for T cells i.e. MHC class I-peptide (pMHC I) complexes for CD8+ T cells and MHC class II-peptide (pMHC II) complexes for CD4+ T cells and flow cytometry. Here we review major developments in the development of pMHC staining reagents and their diverse applications and discuss perspectives of their use for basic and clinical investigations.http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00218/fullFlow CytometryVaccinationT cellsT cell receptorcoreceptorMHC I |
| spellingShingle | Julien eSchmidt Danijel eDojcinovic Philippe eGuillaume Immanuel eLuescher ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES Frontiers in Immunology Flow Cytometry Vaccination T cells T cell receptor coreceptor MHC I |
| title | ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES |
| title_full | ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES |
| title_fullStr | ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES |
| title_full_unstemmed | ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES |
| title_short | ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES |
| title_sort | analysis isolation and activation of antigen specific cd4 and cd8 t cells by soluble mhc peptide complexes |
| topic | Flow Cytometry Vaccination T cells T cell receptor coreceptor MHC I |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00218/full |
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