| Summary: | Key points 1. The increased surface area of PET promotes its hydrolysis by Cut190, supporting the surface erosion mechanism by a two-step process (endo-type scission of a polymer chain and exo-type hydrolysis of depolymerized fragments). 2. Dodecyltrimethylammonium chloride functioned as a binding module between Cut190 and PET surface, showing the higher hydrolysis rate at 65 °C than at 70 °C in the absence of the detergent. 3. The best performing Cut190 variant produced more than 90 mM degradation products at 63 °C in 3 days and approximately 80 mM at 65 °C in one day, using amorphous PET powders. Additionally, polyethylene furanoate was highly hydrolyzed at 63 °C in 3 days by the Cut190 variant.
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