Decellularized bone matrix grafts for calvaria regeneration
Decellularization is a promising new method to prepare natural matrices for tissue regeneration. Successful decellularization has been reported using various tissues including skin, tendon, and cartilage, though studies using hard tissue such as bone are lacking. In this study, we aimed to define th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2016-12-01
|
| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731416680306 |
| _version_ | 1828893720461705216 |
|---|---|
| author | Dong Joon Lee Shannon Diachina Yan Ting Lee Lixing Zhao Rui Zou Na Tang Han Han Xin Chen Ching-Chang Ko |
| author_facet | Dong Joon Lee Shannon Diachina Yan Ting Lee Lixing Zhao Rui Zou Na Tang Han Han Xin Chen Ching-Chang Ko |
| author_sort | Dong Joon Lee |
| collection | DOAJ |
| description | Decellularization is a promising new method to prepare natural matrices for tissue regeneration. Successful decellularization has been reported using various tissues including skin, tendon, and cartilage, though studies using hard tissue such as bone are lacking. In this study, we aimed to define the optimal experimental parameters to decellularize natural bone matrix using 0.5% sodium dodecyl sulfate and 0.1% NH 4 OH. Then, the effects of decellularized bone matrix on rat mesenchymal stem cell proliferation, osteogenic gene expression, and osteogenic differentiations in a two-dimensional culture system were investigated. Decellularized bone was also evaluated with regard to cytotoxicity, biochemical, and mechanical characteristics in vitro. Evidence of complete decellularization was shown through hematoxylin and eosin staining and DNA measurements. Decellularized bone matrix displayed a cytocompatible property, conserved structure, mechanical strength, and mineral content comparable to natural bone. To study new bone formation, implantation of decellularized bone matrix particles seeded with rat mesenchymal stem cells was conducted using an orthotopic in vivo model. After 3 months post-implantation into a critical-sized defect in rat calvaria, new bone was formed around decellularized bone matrix particles and also merged with new bone between decellularized bone matrix particles. New bone formation was analyzed with micro computed tomography, mineral apposition rate, and histomorphometry. Decellularized bone matrix stimulated mesenchymal stem cell proliferation and osteogenic differentiation in vitro and in vivo, achieving effective bone regeneration and thereby serving as a promising biological bone graft. |
| first_indexed | 2024-12-13T13:59:23Z |
| format | Article |
| id | doaj.art-56c6353299a24923a58601b9cdadba77 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-13T13:59:23Z |
| publishDate | 2016-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-56c6353299a24923a58601b9cdadba772022-12-21T23:42:48ZengSAGE PublishingJournal of Tissue Engineering2041-73142016-12-01710.1177/204173141668030610.1177_2041731416680306Decellularized bone matrix grafts for calvaria regenerationDong Joon Lee0Shannon Diachina1Yan Ting Lee2Lixing Zhao3Rui Zou4Na Tang5Han Han6Xin Chen7Ching-Chang Ko8Oral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Sciences Research, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USADepartment of Orthodontics, UNC School of Dentistry, University of North Carolina, Chapel Hill, NC, USADecellularization is a promising new method to prepare natural matrices for tissue regeneration. Successful decellularization has been reported using various tissues including skin, tendon, and cartilage, though studies using hard tissue such as bone are lacking. In this study, we aimed to define the optimal experimental parameters to decellularize natural bone matrix using 0.5% sodium dodecyl sulfate and 0.1% NH 4 OH. Then, the effects of decellularized bone matrix on rat mesenchymal stem cell proliferation, osteogenic gene expression, and osteogenic differentiations in a two-dimensional culture system were investigated. Decellularized bone was also evaluated with regard to cytotoxicity, biochemical, and mechanical characteristics in vitro. Evidence of complete decellularization was shown through hematoxylin and eosin staining and DNA measurements. Decellularized bone matrix displayed a cytocompatible property, conserved structure, mechanical strength, and mineral content comparable to natural bone. To study new bone formation, implantation of decellularized bone matrix particles seeded with rat mesenchymal stem cells was conducted using an orthotopic in vivo model. After 3 months post-implantation into a critical-sized defect in rat calvaria, new bone was formed around decellularized bone matrix particles and also merged with new bone between decellularized bone matrix particles. New bone formation was analyzed with micro computed tomography, mineral apposition rate, and histomorphometry. Decellularized bone matrix stimulated mesenchymal stem cell proliferation and osteogenic differentiation in vitro and in vivo, achieving effective bone regeneration and thereby serving as a promising biological bone graft.https://doi.org/10.1177/2041731416680306 |
| spellingShingle | Dong Joon Lee Shannon Diachina Yan Ting Lee Lixing Zhao Rui Zou Na Tang Han Han Xin Chen Ching-Chang Ko Decellularized bone matrix grafts for calvaria regeneration Journal of Tissue Engineering |
| title | Decellularized bone matrix grafts for calvaria regeneration |
| title_full | Decellularized bone matrix grafts for calvaria regeneration |
| title_fullStr | Decellularized bone matrix grafts for calvaria regeneration |
| title_full_unstemmed | Decellularized bone matrix grafts for calvaria regeneration |
| title_short | Decellularized bone matrix grafts for calvaria regeneration |
| title_sort | decellularized bone matrix grafts for calvaria regeneration |
| url | https://doi.org/10.1177/2041731416680306 |
| work_keys_str_mv | AT dongjoonlee decellularizedbonematrixgraftsforcalvariaregeneration AT shannondiachina decellularizedbonematrixgraftsforcalvariaregeneration AT yantinglee decellularizedbonematrixgraftsforcalvariaregeneration AT lixingzhao decellularizedbonematrixgraftsforcalvariaregeneration AT ruizou decellularizedbonematrixgraftsforcalvariaregeneration AT natang decellularizedbonematrixgraftsforcalvariaregeneration AT hanhan decellularizedbonematrixgraftsforcalvariaregeneration AT xinchen decellularizedbonematrixgraftsforcalvariaregeneration AT chingchangko decellularizedbonematrixgraftsforcalvariaregeneration |