Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study
The assessment of alteration of postmortem RNA expression has forensic significance in estimating postmortem interval. To evaluate wound healing progression and the effect of different postmortem intervals, histopathological changes, immunohistochemical matrix metalloproteinase-9 (MMP-9) expression,...
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MDPI AG
2022-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/11/2919 |
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| author | Mona M. Ali Samah F. Ibrahim Noha M. Elrewieny Aya M. Elyamany Wagdy K. B. Khalil Aziza B. Shalby Sarah A. Khater |
| author_facet | Mona M. Ali Samah F. Ibrahim Noha M. Elrewieny Aya M. Elyamany Wagdy K. B. Khalil Aziza B. Shalby Sarah A. Khater |
| author_sort | Mona M. Ali |
| collection | DOAJ |
| description | The assessment of alteration of postmortem RNA expression has forensic significance in estimating postmortem interval. To evaluate wound healing progression and the effect of different postmortem intervals, histopathological changes, immunohistochemical matrix metalloproteinase-9 (MMP-9) expression, and long noncoding fatty acid oxidation (lncFAO), RNA expression was assessed in the incised cutaneous wound model. A full-thickness cutaneous wound was inflicted on 75 rats. All 15 rats were sacrificed at different post-infliction intervals (0, 2, 4, 8 and 10 days), and the cutaneous wounds (<i>n</i> = 5) were excised at different postmortem intervals (0, 5, and 24 h after euthanasia). The maximal inflammatory healing stage was detected at day 4 post-infliction, while near complete healing, thick mature collagen deposition was detected at day 10 post-infliction. LncFAO expression was significantly over-expressed with increasing wound age. MMP-9 was detectable on injury day with continuous elevation until 8 days post-wounding, which later decreased. Although histopathological and immunohistochemical examinations within 24 h postmortem did not show any remarkable changes, lncFAO RNA expression showed a significant negative correlation with hours passed since death. The combined use of histopathological changes, immunohistochemical expression of MMP-9, and molecular expression of lncFAO could be appropriate in wound dating verification. Among these factors, lncFAO could be a reliable indicator in postmortem interval estimation. |
| first_indexed | 2024-03-09T18:27:17Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T18:27:17Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-56c9f114f8224bf6afb5a8f3d9c9d1692023-11-24T07:46:12ZengMDPI AGBiomedicines2227-90592022-11-011011291910.3390/biomedicines10112919Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental StudyMona M. Ali0Samah F. Ibrahim1Noha M. Elrewieny2Aya M. Elyamany3Wagdy K. B. Khalil4Aziza B. Shalby5Sarah A. Khater6Forensic Medicine and Clinical Toxicology Department, College of Medicine, Cairo University, Cairo 11956, EgyptClinical Sciences Department, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaForensic Medicine and Clinical Toxicology Department, College of Medicine, Cairo University, Cairo 11956, EgyptPathology Department, College of Medicine, Cairo University, Cairo 11956, EgyptCell Biology Department, Biotechnology Research Institute, National Research Center, Cairo P.O. Box 12622, EgyptHormones Department, National Research Center, Cairo P.O. Box 12622, EgyptForensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Misr University for Science and Technology, 6th October, P.O. Box 77, Cairo 3236101, EgyptThe assessment of alteration of postmortem RNA expression has forensic significance in estimating postmortem interval. To evaluate wound healing progression and the effect of different postmortem intervals, histopathological changes, immunohistochemical matrix metalloproteinase-9 (MMP-9) expression, and long noncoding fatty acid oxidation (lncFAO), RNA expression was assessed in the incised cutaneous wound model. A full-thickness cutaneous wound was inflicted on 75 rats. All 15 rats were sacrificed at different post-infliction intervals (0, 2, 4, 8 and 10 days), and the cutaneous wounds (<i>n</i> = 5) were excised at different postmortem intervals (0, 5, and 24 h after euthanasia). The maximal inflammatory healing stage was detected at day 4 post-infliction, while near complete healing, thick mature collagen deposition was detected at day 10 post-infliction. LncFAO expression was significantly over-expressed with increasing wound age. MMP-9 was detectable on injury day with continuous elevation until 8 days post-wounding, which later decreased. Although histopathological and immunohistochemical examinations within 24 h postmortem did not show any remarkable changes, lncFAO RNA expression showed a significant negative correlation with hours passed since death. The combined use of histopathological changes, immunohistochemical expression of MMP-9, and molecular expression of lncFAO could be appropriate in wound dating verification. Among these factors, lncFAO could be a reliable indicator in postmortem interval estimation.https://www.mdpi.com/2227-9059/10/11/2919forensic medicineincised woundpostmortem intervallong non-coding RNAmatrix metalloproteinase-9 |
| spellingShingle | Mona M. Ali Samah F. Ibrahim Noha M. Elrewieny Aya M. Elyamany Wagdy K. B. Khalil Aziza B. Shalby Sarah A. Khater Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study Biomedicines forensic medicine incised wound postmortem interval long non-coding RNA matrix metalloproteinase-9 |
| title | Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study |
| title_full | Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study |
| title_fullStr | Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study |
| title_full_unstemmed | Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study |
| title_short | Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study |
| title_sort | estimation of early postmortem interval from long noncoding rna gene expression in the incised cutaneous wound an experimental study |
| topic | forensic medicine incised wound postmortem interval long non-coding RNA matrix metalloproteinase-9 |
| url | https://www.mdpi.com/2227-9059/10/11/2919 |
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