Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it>
<p>Abstract</p> <p>Background</p> <p>Serine proteases (SPs) and serine proteases homologs (SPHs) are a large group of proteolytic enzymes, with important roles in a variety of physiological processes, such as cell signalling, defense and development. Genome-wide identif...
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| Format: | Article |
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BMC
2010-06-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/405 |
| _version_ | 1818473277111140352 |
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| author | Xu Ping-Zhen Duan Jun Dong Zhao-Ming Wang Gen-Hong Zhao Ping Cheng Ting-Cai Xiang Zhong-Huai Xia Qing-You |
| author_facet | Xu Ping-Zhen Duan Jun Dong Zhao-Ming Wang Gen-Hong Zhao Ping Cheng Ting-Cai Xiang Zhong-Huai Xia Qing-You |
| author_sort | Xu Ping-Zhen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Serine proteases (SPs) and serine proteases homologs (SPHs) are a large group of proteolytic enzymes, with important roles in a variety of physiological processes, such as cell signalling, defense and development. Genome-wide identification and expression analysis of serine proteases and their homologs in the silkworm might provide valuable information about their biological functions.</p> <p>Results</p> <p>In this study, 51 SP genes and 92 SPH genes were systematically identified in the genome of the silkworm <it>Bombyx mori</it>. Phylogenetic analysis indicated that six gene families have been amplified species-specifically in the silkworm, and the members of them showed chromosomal distribution of tandem repeats. Microarray analysis suggests that many silkworm-specific genes, such as members of SP_fam12, 13, 14 and 15, show expression patterns that are specific to tissues or developmental stages. The roles of SPs and SPHs in resisting pathogens were investigated in silkworms when they were infected by <it>Escherichia coli</it>, <it>Bacillus bombysepticus</it>, <it>Batrytis bassiana </it>and <it>B. mori </it><it>nucleopolyhedrovirus</it>, respectively. Microarray experiment and real-time quantitative RT-PCR showed that 18 SP or SPH genes were significantly up-regulated after pathogen induction, suggesting that SP and SPH genes might participate in pathogenic microorganism resistance in <it>B. mori</it>.</p> <p>Conclusion</p> <p>Silkworm SP and SPH genes were identified. Comparative genomics showed that SP and SPH genes belong to a large family, whose members are generated mainly by tandem repeat evolution. We found that silkworm has species-specific SP and SPH genes. Phylogenetic and microarray analyses provide an overview of the silkworm SP and SPHs, and facilitate future functional studies on these enzymes.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-14T04:21:52Z |
| publishDate | 2010-06-01 |
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| spelling | doaj.art-56ca9bbfcf444817bfc1e9af5900179d2022-12-22T02:12:30ZengBMCBMC Genomics1471-21642010-06-0111140510.1186/1471-2164-11-405Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it>Xu Ping-ZhenDuan JunDong Zhao-MingWang Gen-HongZhao PingCheng Ting-CaiXiang Zhong-HuaiXia Qing-You<p>Abstract</p> <p>Background</p> <p>Serine proteases (SPs) and serine proteases homologs (SPHs) are a large group of proteolytic enzymes, with important roles in a variety of physiological processes, such as cell signalling, defense and development. Genome-wide identification and expression analysis of serine proteases and their homologs in the silkworm might provide valuable information about their biological functions.</p> <p>Results</p> <p>In this study, 51 SP genes and 92 SPH genes were systematically identified in the genome of the silkworm <it>Bombyx mori</it>. Phylogenetic analysis indicated that six gene families have been amplified species-specifically in the silkworm, and the members of them showed chromosomal distribution of tandem repeats. Microarray analysis suggests that many silkworm-specific genes, such as members of SP_fam12, 13, 14 and 15, show expression patterns that are specific to tissues or developmental stages. The roles of SPs and SPHs in resisting pathogens were investigated in silkworms when they were infected by <it>Escherichia coli</it>, <it>Bacillus bombysepticus</it>, <it>Batrytis bassiana </it>and <it>B. mori </it><it>nucleopolyhedrovirus</it>, respectively. Microarray experiment and real-time quantitative RT-PCR showed that 18 SP or SPH genes were significantly up-regulated after pathogen induction, suggesting that SP and SPH genes might participate in pathogenic microorganism resistance in <it>B. mori</it>.</p> <p>Conclusion</p> <p>Silkworm SP and SPH genes were identified. Comparative genomics showed that SP and SPH genes belong to a large family, whose members are generated mainly by tandem repeat evolution. We found that silkworm has species-specific SP and SPH genes. Phylogenetic and microarray analyses provide an overview of the silkworm SP and SPHs, and facilitate future functional studies on these enzymes.</p>http://www.biomedcentral.com/1471-2164/11/405 |
| spellingShingle | Xu Ping-Zhen Duan Jun Dong Zhao-Ming Wang Gen-Hong Zhao Ping Cheng Ting-Cai Xiang Zhong-Huai Xia Qing-You Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it> BMC Genomics |
| title | Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it> |
| title_full | Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it> |
| title_fullStr | Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it> |
| title_full_unstemmed | Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it> |
| title_short | Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm <it>Bombyx mori</it> |
| title_sort | genome wide identification and expression analysis of serine proteases and homologs in the silkworm it bombyx mori it |
| url | http://www.biomedcentral.com/1471-2164/11/405 |
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