RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification
Seed dormancy is an adaptive strategy for environmental evolution. However, the molecular mechanism of the breaking of seed dormancy at cold temperatures is still unclear, and the genetic regulation of germination initiated by exposure to cold temperature requires further investigation. In the initi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1021572/full |
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| author | Qiu-Xiong Yang Qiu-Xiong Yang Qiu-Xiong Yang Dan Chen Dan Chen Dan Chen Yan Zhao Yan Zhao Yan Zhao Xiao-Yu Zhang Xiao-Yu Zhang Xiao-Yu Zhang Min Zhao Min Zhao Min Zhao Rui Peng Nian-Xi Sun Timothy Charles Baldwin Sheng-Chao Yang Sheng-Chao Yang Sheng-Chao Yang Yan-Li Liang Yan-Li Liang Yan-Li Liang |
| author_facet | Qiu-Xiong Yang Qiu-Xiong Yang Qiu-Xiong Yang Dan Chen Dan Chen Dan Chen Yan Zhao Yan Zhao Yan Zhao Xiao-Yu Zhang Xiao-Yu Zhang Xiao-Yu Zhang Min Zhao Min Zhao Min Zhao Rui Peng Nian-Xi Sun Timothy Charles Baldwin Sheng-Chao Yang Sheng-Chao Yang Sheng-Chao Yang Yan-Li Liang Yan-Li Liang Yan-Li Liang |
| author_sort | Qiu-Xiong Yang |
| collection | DOAJ |
| description | Seed dormancy is an adaptive strategy for environmental evolution. However, the molecular mechanism of the breaking of seed dormancy at cold temperatures is still unclear, and the genetic regulation of germination initiated by exposure to cold temperature requires further investigation. In the initial phase of the current study, the seed coat characteristics and embryo development of Fritillaria taipaiensis P.Y.Li at different temperatures (0°C, 4°C, 10°C & 25°C) was recorded. The results obtained demonstrated that embryo elongation and the dormancy-breaking was most significantly affected at 4°C. Subsequently, transcriptome analyses of seeds in different states of dormancy, at two stratification temperatures (4°C and 25°C) was performed, combined with weighted gene coexpression network analysis (WGCNA) and metabolomics, to explore the transcriptional regulation of seed germination in F. taipaiensis at the two selected stratification temperatures. The results showed that stratification at the colder temperature (4°C) induced an up-regulation of gene expression involved in gibberellic acid (GA) and auxin biosynthesis and the down-regulation of genes related to the abscisic acid (ABA) biosynthetic pathway. Thereby promoting embryo development and the stimulation of seed germination. Collectively, these data constitute a significant advance in our understanding of the role of cold temperatures in the regulation of seed germination in F. taipaiensis and also provide valuable transcriptomic data for seed dormancy for other non-model plant species. |
| first_indexed | 2024-04-12T03:43:08Z |
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| id | doaj.art-56cae9beb6464bc2897d79d3e6d9c765 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T03:43:08Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-56cae9beb6464bc2897d79d3e6d9c7652022-12-22T03:49:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10215721021572RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratificationQiu-Xiong Yang0Qiu-Xiong Yang1Qiu-Xiong Yang2Dan Chen3Dan Chen4Dan Chen5Yan Zhao6Yan Zhao7Yan Zhao8Xiao-Yu Zhang9Xiao-Yu Zhang10Xiao-Yu Zhang11Min Zhao12Min Zhao13Min Zhao14Rui Peng15Nian-Xi Sun16Timothy Charles Baldwin17Sheng-Chao Yang18Sheng-Chao Yang19Sheng-Chao Yang20Yan-Li Liang21Yan-Li Liang22Yan-Li Liang23College of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaChongqing Academy of Chinese Materia Medica, Chongqing, ChinaChongqing Academy of Chinese Materia Medica, Chongqing, ChinaFaculty of Science and Engineering, University of Wolverhampton, Wolverhampton, United KingdomCollege of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy & Biotechnology, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural Waseda University, Fengyuan, Kunming, ChinaNational & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, ChinaSeed dormancy is an adaptive strategy for environmental evolution. However, the molecular mechanism of the breaking of seed dormancy at cold temperatures is still unclear, and the genetic regulation of germination initiated by exposure to cold temperature requires further investigation. In the initial phase of the current study, the seed coat characteristics and embryo development of Fritillaria taipaiensis P.Y.Li at different temperatures (0°C, 4°C, 10°C & 25°C) was recorded. The results obtained demonstrated that embryo elongation and the dormancy-breaking was most significantly affected at 4°C. Subsequently, transcriptome analyses of seeds in different states of dormancy, at two stratification temperatures (4°C and 25°C) was performed, combined with weighted gene coexpression network analysis (WGCNA) and metabolomics, to explore the transcriptional regulation of seed germination in F. taipaiensis at the two selected stratification temperatures. The results showed that stratification at the colder temperature (4°C) induced an up-regulation of gene expression involved in gibberellic acid (GA) and auxin biosynthesis and the down-regulation of genes related to the abscisic acid (ABA) biosynthetic pathway. Thereby promoting embryo development and the stimulation of seed germination. Collectively, these data constitute a significant advance in our understanding of the role of cold temperatures in the regulation of seed germination in F. taipaiensis and also provide valuable transcriptomic data for seed dormancy for other non-model plant species.https://www.frontiersin.org/articles/10.3389/fpls.2022.1021572/fullseed dormancy4°C stratificationRNA-seqphytohormoneWGCNAfritillaria taipaiensis P.Y.Li |
| spellingShingle | Qiu-Xiong Yang Qiu-Xiong Yang Qiu-Xiong Yang Dan Chen Dan Chen Dan Chen Yan Zhao Yan Zhao Yan Zhao Xiao-Yu Zhang Xiao-Yu Zhang Xiao-Yu Zhang Min Zhao Min Zhao Min Zhao Rui Peng Nian-Xi Sun Timothy Charles Baldwin Sheng-Chao Yang Sheng-Chao Yang Sheng-Chao Yang Yan-Li Liang Yan-Li Liang Yan-Li Liang RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification Frontiers in Plant Science seed dormancy 4°C stratification RNA-seq phytohormone WGCNA fritillaria taipaiensis P.Y.Li |
| title | RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification |
| title_full | RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification |
| title_fullStr | RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification |
| title_full_unstemmed | RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification |
| title_short | RNA-seq analysis reveals key genes associated with seed germination of Fritillaria taipaiensis P.Y.Li by cold stratification |
| title_sort | rna seq analysis reveals key genes associated with seed germination of fritillaria taipaiensis p y li by cold stratification |
| topic | seed dormancy 4°C stratification RNA-seq phytohormone WGCNA fritillaria taipaiensis P.Y.Li |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1021572/full |
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