Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>)
Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/2/387 |
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| author | Douglas Jardim-Messeder Andreia Caverzan Natalia Balbinott Paloma K. Menguer Ana L. S. Paiva Moaciria Lemos Juliana R. Cunha Marcos L. Gaeta Miguel Costa Marcel Zamocky Nelson J. M. Saibo Joaquim A. G. Silveira Rogério Margis Márcia Margis-Pinheiro |
| author_facet | Douglas Jardim-Messeder Andreia Caverzan Natalia Balbinott Paloma K. Menguer Ana L. S. Paiva Moaciria Lemos Juliana R. Cunha Marcos L. Gaeta Miguel Costa Marcel Zamocky Nelson J. M. Saibo Joaquim A. G. Silveira Rogério Margis Márcia Margis-Pinheiro |
| author_sort | Douglas Jardim-Messeder |
| collection | DOAJ |
| description | Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did not impact plant growth, redox state, or photosynthesis parameters. Nevertheless, when subjected to drought stress, silenced plants (APX7i) show a higher capacity to maintain stomata aperture and photosynthesis performance, resulting in a higher tolerance when compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 did not lead to changes in the global expression of genes related to reactive oxygen species metabolism. In addition, the drought-mediated induction of several genes related to the proteasome pathway and the down-regulation of genes related to nitrogen and carotenoid metabolism was impaired in APX7i plants. During drought stress, APX7i showed an up-regulation of genes encoding flavonoid and tyrosine metabolism enzymes and a down-regulation of genes related to phytohormones signal transduction and nicotinate and nicotinamide metabolism. Our results demonstrate that OsAPX7 might be involved in signaling transduction pathways related to drought stress response, contributing to the understanding of the physiological role of chloroplast APX isoforms in rice. |
| first_indexed | 2024-03-11T09:13:57Z |
| format | Article |
| id | doaj.art-56cce345fd5e4bcb95c4e62754c341ad |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T09:13:57Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-56cce345fd5e4bcb95c4e62754c341ad2023-11-16T18:47:28ZengMDPI AGAntioxidants2076-39212023-02-0112238710.3390/antiox12020387Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>)Douglas Jardim-Messeder0Andreia Caverzan1Natalia Balbinott2Paloma K. Menguer3Ana L. S. Paiva4Moaciria Lemos5Juliana R. Cunha6Marcos L. Gaeta7Miguel Costa8Marcel Zamocky9Nelson J. M. Saibo10Joaquim A. G. Silveira11Rogério Margis12Márcia Margis-Pinheiro13Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilCentro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilCentro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 60020-181, CE, BrazilDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 60020-181, CE, BrazilDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 60020-181, CE, BrazilDepartamento de Botânica, Universidade Federal Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilLEAF, TERRA, Instituto Superior de Agronomia, University of Lisbon, 1349-017 Lisboa, PortugalLaboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551 Bratislava, SlovakiaInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, PortugalDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza 60020-181, CE, BrazilDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre 90010-150, RS, BrazilChloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did not impact plant growth, redox state, or photosynthesis parameters. Nevertheless, when subjected to drought stress, silenced plants (APX7i) show a higher capacity to maintain stomata aperture and photosynthesis performance, resulting in a higher tolerance when compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 did not lead to changes in the global expression of genes related to reactive oxygen species metabolism. In addition, the drought-mediated induction of several genes related to the proteasome pathway and the down-regulation of genes related to nitrogen and carotenoid metabolism was impaired in APX7i plants. During drought stress, APX7i showed an up-regulation of genes encoding flavonoid and tyrosine metabolism enzymes and a down-regulation of genes related to phytohormones signal transduction and nicotinate and nicotinamide metabolism. Our results demonstrate that OsAPX7 might be involved in signaling transduction pathways related to drought stress response, contributing to the understanding of the physiological role of chloroplast APX isoforms in rice.https://www.mdpi.com/2076-3921/12/2/387ascorbate peroxidasereactive oxygen specieschloroplastabiotic stressdroughtplant stress response |
| spellingShingle | Douglas Jardim-Messeder Andreia Caverzan Natalia Balbinott Paloma K. Menguer Ana L. S. Paiva Moaciria Lemos Juliana R. Cunha Marcos L. Gaeta Miguel Costa Marcel Zamocky Nelson J. M. Saibo Joaquim A. G. Silveira Rogério Margis Márcia Margis-Pinheiro Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>) Antioxidants ascorbate peroxidase reactive oxygen species chloroplast abiotic stress drought plant stress response |
| title | Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>) |
| title_full | Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>) |
| title_fullStr | Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>) |
| title_full_unstemmed | Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>) |
| title_short | Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>) |
| title_sort | stromal ascorbate peroxidase i osapx7 i modulates drought stress tolerance in rice i oryza sativa i |
| topic | ascorbate peroxidase reactive oxygen species chloroplast abiotic stress drought plant stress response |
| url | https://www.mdpi.com/2076-3921/12/2/387 |
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