Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation
No radical treatment is available for the regeneration of dysfunction and defects in airway epithelia. Artificial tracheae made of polypropylene and collagen sponge were used in clinical studies to reconstitute tracheae after resection. For early epithelialization of the luminal surface of the artif...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2024-02-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897241228026 |
| _version_ | 1797302273765801984 |
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| author | Masayuki Kitano Yasuyuki Hayashi Hiroe Ohnishi Hideaki Okuyama Masayoshi Yoshimatsu Keisuke Mizuno Fumihiko Kuwata Takeshi Tada Yo Kishimoto Satoshi Morita Koichi Omori |
| author_facet | Masayuki Kitano Yasuyuki Hayashi Hiroe Ohnishi Hideaki Okuyama Masayoshi Yoshimatsu Keisuke Mizuno Fumihiko Kuwata Takeshi Tada Yo Kishimoto Satoshi Morita Koichi Omori |
| author_sort | Masayuki Kitano |
| collection | DOAJ |
| description | No radical treatment is available for the regeneration of dysfunction and defects in airway epithelia. Artificial tracheae made of polypropylene and collagen sponge were used in clinical studies to reconstitute tracheae after resection. For early epithelialization of the luminal surface of the artificial trachea, a model was established, that is, an artificial trachea covered with human-induced pluripotent stem cell-derived airway epithelial cells (hiPSC-AECs) was transplanted into a tracheal defect in an immunodeficient rat. Unlike the cell types of hiPSC-derived cells that are currently used in clinical studies, AECs maintain tissues by proliferation and differentiation of basal cells into various cell types that constitute AECs constantly. Therefore, post-transplantation, the proportion of each cell type, such as ciliated and goblet cells, may change; however, no studies have examined this possibility. In this study, using our hiPSC-AEC-transplanted rat model, we investigated changes in the proportion of each cell type in hiPSC-AECs pre-transplantation and post-transplantation. As a result, the proportion of each cell type changed post-transplantation. The proportion of ciliated, basal, and club cells increased, and the proportion of goblet cells decreased post-transplantation. In addition, the proportion of each cell type in engrafted hiPSC-AECs is more similar to the proportion of each cell type in normal proximal airway tissue than the proportion of each cell type pre-transplantation. The results of this study are useful for the development of therapeutic techniques using hiPSC-AEC transplantation. |
| first_indexed | 2024-03-07T23:34:25Z |
| format | Article |
| id | doaj.art-56ceaf31fb5d41f690a5ce87792e68d5 |
| institution | Directory Open Access Journal |
| issn | 1555-3892 |
| language | English |
| last_indexed | 2024-03-07T23:34:25Z |
| publishDate | 2024-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-56ceaf31fb5d41f690a5ce87792e68d52024-02-20T09:03:33ZengSAGE PublishingCell Transplantation1555-38922024-02-013310.1177/09636897241228026Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia TransplantationMasayuki Kitano0Yasuyuki Hayashi1Hiroe Ohnishi2Hideaki Okuyama3Masayoshi Yoshimatsu4Keisuke Mizuno5Fumihiko Kuwata6Takeshi Tada7Yo Kishimoto8Satoshi Morita9Koichi Omori10Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanSchool of Communication Sciences and Disorders, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, CanadaDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, JapanDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanCenter for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USADepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDepartment of Biomedical Statistics and Bioinformatics, Graduate School of Medicine, Kyoto University, Kyoto, JapanDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanNo radical treatment is available for the regeneration of dysfunction and defects in airway epithelia. Artificial tracheae made of polypropylene and collagen sponge were used in clinical studies to reconstitute tracheae after resection. For early epithelialization of the luminal surface of the artificial trachea, a model was established, that is, an artificial trachea covered with human-induced pluripotent stem cell-derived airway epithelial cells (hiPSC-AECs) was transplanted into a tracheal defect in an immunodeficient rat. Unlike the cell types of hiPSC-derived cells that are currently used in clinical studies, AECs maintain tissues by proliferation and differentiation of basal cells into various cell types that constitute AECs constantly. Therefore, post-transplantation, the proportion of each cell type, such as ciliated and goblet cells, may change; however, no studies have examined this possibility. In this study, using our hiPSC-AEC-transplanted rat model, we investigated changes in the proportion of each cell type in hiPSC-AECs pre-transplantation and post-transplantation. As a result, the proportion of each cell type changed post-transplantation. The proportion of ciliated, basal, and club cells increased, and the proportion of goblet cells decreased post-transplantation. In addition, the proportion of each cell type in engrafted hiPSC-AECs is more similar to the proportion of each cell type in normal proximal airway tissue than the proportion of each cell type pre-transplantation. The results of this study are useful for the development of therapeutic techniques using hiPSC-AEC transplantation.https://doi.org/10.1177/09636897241228026 |
| spellingShingle | Masayuki Kitano Yasuyuki Hayashi Hiroe Ohnishi Hideaki Okuyama Masayoshi Yoshimatsu Keisuke Mizuno Fumihiko Kuwata Takeshi Tada Yo Kishimoto Satoshi Morita Koichi Omori Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation Cell Transplantation |
| title | Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation |
| title_full | Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation |
| title_fullStr | Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation |
| title_full_unstemmed | Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation |
| title_short | Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation |
| title_sort | changes in the proportion of each cell type after hipsc derived airway epithelia transplantation |
| url | https://doi.org/10.1177/09636897241228026 |
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