Canstatin represses glioma growth by inhibiting formation of VM-like structures
Vasculogenic mimicry (VM) is different from classical tumor angiogenesis and does not depend on endothelial cells. VM is closely related to the prognosis of various cancers. Canstatin was first identified as an endogenous angiogenesis inhibitor. In the present study, the inhibitory effect of canstat...
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-08-01
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| Series: | Translational Neuroscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/tnsci-2020-0176 |
| _version_ | 1811185979346649088 |
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| author | Ma Yuqiang Wu Tao Zhou Houjie He Guilu Li Yifei Wang Bocheng Guo Qiang Chen Baodong Li Weiping |
| author_facet | Ma Yuqiang Wu Tao Zhou Houjie He Guilu Li Yifei Wang Bocheng Guo Qiang Chen Baodong Li Weiping |
| author_sort | Ma Yuqiang |
| collection | DOAJ |
| description | Vasculogenic mimicry (VM) is different from classical tumor angiogenesis and does not depend on endothelial cells. VM is closely related to the prognosis of various cancers. Canstatin was first identified as an endogenous angiogenesis inhibitor. In the present study, the inhibitory effect of canstatin on VM formation was evaluated. Human glioblastoma cell lines U87 and U251 were letivirally transduced to overexpress canstatin gene or GFP as control. In vitro assays showed that canstatin overexpression reduced the tube formation of U87 and U251 cells in Matrigel. A xenograft glioma model was created by subcutaneous injection of lentivirally modified U87 cells into nude mice. The results of in vivo experiments showed that canstatin gene introduction inhibited the growth of glioma xenografts. In tumor xenografts overexpressing canstatin, U87-mediated formation of VM-like structures and VM-related VEGF (vascular endothelial growth factor) expression were remarkably reduced. Canstatin overexpression also decreased the phosphorylation of Akt and reduced the expression of Survivin in vitro. In addition, HIF-1α production and MMP-2 secretion were decreased by canstatin overexpression. Therefore, these results suggested a protective role of canstatin during VM-like structure formation of glioma probably via inhibiting signaling pathways inducing vasculogenic mimicry. |
| first_indexed | 2024-04-11T13:38:37Z |
| format | Article |
| id | doaj.art-56cf1f4e838a44e8a0f2771f6e03528a |
| institution | Directory Open Access Journal |
| issn | 2081-6936 |
| language | English |
| last_indexed | 2024-04-11T13:38:37Z |
| publishDate | 2021-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Translational Neuroscience |
| spelling | doaj.art-56cf1f4e838a44e8a0f2771f6e03528a2022-12-22T04:21:21ZengDe GruyterTranslational Neuroscience2081-69362021-08-0112130931910.1515/tnsci-2020-0176Canstatin represses glioma growth by inhibiting formation of VM-like structuresMa Yuqiang0Wu Tao1Zhou Houjie2He Guilu3Li Yifei4Wang Bocheng5Guo Qiang6Chen Baodong7Li Weiping8Neurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaNeurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaNeurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaGuangDong 999 Brain Hospital, Guangzhou 510510, People’s Republic of ChinaNeurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaNeurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaNeurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaNeurosurgery Department, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen 518035, People’s Republic of ChinaNeurosurgery Department, Shenzhen Second People’s Hospital, Sungang Road, Futian District, Guangzhou 510510, People’s Republic of ChinaVasculogenic mimicry (VM) is different from classical tumor angiogenesis and does not depend on endothelial cells. VM is closely related to the prognosis of various cancers. Canstatin was first identified as an endogenous angiogenesis inhibitor. In the present study, the inhibitory effect of canstatin on VM formation was evaluated. Human glioblastoma cell lines U87 and U251 were letivirally transduced to overexpress canstatin gene or GFP as control. In vitro assays showed that canstatin overexpression reduced the tube formation of U87 and U251 cells in Matrigel. A xenograft glioma model was created by subcutaneous injection of lentivirally modified U87 cells into nude mice. The results of in vivo experiments showed that canstatin gene introduction inhibited the growth of glioma xenografts. In tumor xenografts overexpressing canstatin, U87-mediated formation of VM-like structures and VM-related VEGF (vascular endothelial growth factor) expression were remarkably reduced. Canstatin overexpression also decreased the phosphorylation of Akt and reduced the expression of Survivin in vitro. In addition, HIF-1α production and MMP-2 secretion were decreased by canstatin overexpression. Therefore, these results suggested a protective role of canstatin during VM-like structure formation of glioma probably via inhibiting signaling pathways inducing vasculogenic mimicry.https://doi.org/10.1515/tnsci-2020-0176gliomacanstatinvasculogenic mimicryu87vegfhif-1α |
| spellingShingle | Ma Yuqiang Wu Tao Zhou Houjie He Guilu Li Yifei Wang Bocheng Guo Qiang Chen Baodong Li Weiping Canstatin represses glioma growth by inhibiting formation of VM-like structures Translational Neuroscience glioma canstatin vasculogenic mimicry u87 vegf hif-1α |
| title | Canstatin represses glioma growth by inhibiting formation of VM-like structures |
| title_full | Canstatin represses glioma growth by inhibiting formation of VM-like structures |
| title_fullStr | Canstatin represses glioma growth by inhibiting formation of VM-like structures |
| title_full_unstemmed | Canstatin represses glioma growth by inhibiting formation of VM-like structures |
| title_short | Canstatin represses glioma growth by inhibiting formation of VM-like structures |
| title_sort | canstatin represses glioma growth by inhibiting formation of vm like structures |
| topic | glioma canstatin vasculogenic mimicry u87 vegf hif-1α |
| url | https://doi.org/10.1515/tnsci-2020-0176 |
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