Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events
<i>Background:</i> Lung cancer (LC) is a major leading cause of death worldwide. Immunomodulators that target several immune mechanisms have proven to reduce tumor burden in experimental models through induction of the immune microenvironment. We hypothesized that other biological mechan...
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MDPI AG
2019-09-01
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Series: | Cancers |
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Online Access: | https://www.mdpi.com/2072-6694/11/9/1301 |
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author | Jun Tang Daniel Ramis-Cabrer Xuejie Wang Esther Barreiro |
author_facet | Jun Tang Daniel Ramis-Cabrer Xuejie Wang Esther Barreiro |
author_sort | Jun Tang |
collection | DOAJ |
description | <i>Background:</i> Lung cancer (LC) is a major leading cause of death worldwide. Immunomodulators that target several immune mechanisms have proven to reduce tumor burden in experimental models through induction of the immune microenvironment. We hypothesized that other biological mechanisms may also favor tumor burden reduction in lung cancer-bearing mice treated with immunomodulators. <i>Methods:</i> Tumor weight, area, T cells and tumor growth (immunohistochemistry), oxidative stress, apoptosis, autophagy, and signaling (NF-κB and sirtuin-1) markers were analyzed (immunoblotting) in subcutaneous tumor of BALB/c mice injected with LP07 adenocarcinoma cells treated with monoclonal antibodies (CD-137, CTLA-4, PD-1, and CD-19, <i>N</i> = 9/group) and non-treated control animals. <i>Results:</i> Compared to non-treated cancer mice, in tumors of monoclonal-treated animals, tumor area and weight and ki-67 were significantly reduced, while T cell counts, oxidative stress, apoptosis, autophagy, activated p65, and sirtuin-1 markers were increased. <i>Conclusions:</i> Immunomodulators elicited a reduction in tumor burden (reduced tumor size and weight) through decreased tumor proliferation and increased oxidative stress, apoptosis, autophagy, and signaling markers, which may have interfered with the immune profile of the tumor microenvironment. Future research should be devoted to the elucidation of the specific contribution of each biological mechanism to the reduced tumor burden. |
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issn | 2072-6694 |
language | English |
last_indexed | 2024-03-12T07:11:06Z |
publishDate | 2019-09-01 |
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series | Cancers |
spelling | doaj.art-56d2eef0a7b148cf851cbd5eb3b249852023-09-02T23:07:29ZengMDPI AGCancers2072-66942019-09-01119130110.3390/cancers11091301cancers11091301Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological EventsJun Tang0Daniel Ramis-Cabrer1Xuejie Wang2Esther Barreiro3Pulmonology Department-Muscle Wasting & Cachexia in Chronic Respiratory Diseases & Lung Cancer Research Group, IMIM-<i>Hospital del Mar, Parc de Salut Mar</i>, Biomedical Research Park (PRBB), C/Dr. Aiguader, 88, E-08003 Barcelona, SpainPulmonology Department-Muscle Wasting & Cachexia in Chronic Respiratory Diseases & Lung Cancer Research Group, IMIM-<i>Hospital del Mar, Parc de Salut Mar</i>, Biomedical Research Park (PRBB), C/Dr. Aiguader, 88, E-08003 Barcelona, SpainPulmonology Department-Muscle Wasting & Cachexia in Chronic Respiratory Diseases & Lung Cancer Research Group, IMIM-<i>Hospital del Mar, Parc de Salut Mar</i>, Biomedical Research Park (PRBB), C/Dr. Aiguader, 88, E-08003 Barcelona, SpainPulmonology Department-Muscle Wasting & Cachexia in Chronic Respiratory Diseases & Lung Cancer Research Group, IMIM-<i>Hospital del Mar, Parc de Salut Mar</i>, Biomedical Research Park (PRBB), C/Dr. Aiguader, 88, E-08003 Barcelona, Spain<i>Background:</i> Lung cancer (LC) is a major leading cause of death worldwide. Immunomodulators that target several immune mechanisms have proven to reduce tumor burden in experimental models through induction of the immune microenvironment. We hypothesized that other biological mechanisms may also favor tumor burden reduction in lung cancer-bearing mice treated with immunomodulators. <i>Methods:</i> Tumor weight, area, T cells and tumor growth (immunohistochemistry), oxidative stress, apoptosis, autophagy, and signaling (NF-κB and sirtuin-1) markers were analyzed (immunoblotting) in subcutaneous tumor of BALB/c mice injected with LP07 adenocarcinoma cells treated with monoclonal antibodies (CD-137, CTLA-4, PD-1, and CD-19, <i>N</i> = 9/group) and non-treated control animals. <i>Results:</i> Compared to non-treated cancer mice, in tumors of monoclonal-treated animals, tumor area and weight and ki-67 were significantly reduced, while T cell counts, oxidative stress, apoptosis, autophagy, activated p65, and sirtuin-1 markers were increased. <i>Conclusions:</i> Immunomodulators elicited a reduction in tumor burden (reduced tumor size and weight) through decreased tumor proliferation and increased oxidative stress, apoptosis, autophagy, and signaling markers, which may have interfered with the immune profile of the tumor microenvironment. Future research should be devoted to the elucidation of the specific contribution of each biological mechanism to the reduced tumor burden.https://www.mdpi.com/2072-6694/11/9/1301experimental lung cancerimmunomodulatorsoxidative stressautophagytumor growthsirtuin-1 |
spellingShingle | Jun Tang Daniel Ramis-Cabrer Xuejie Wang Esther Barreiro Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events Cancers experimental lung cancer immunomodulators oxidative stress autophagy tumor growth sirtuin-1 |
title | Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events |
title_full | Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events |
title_fullStr | Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events |
title_full_unstemmed | Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events |
title_short | Immunotherapy with Monoclonal Antibodies in Lung Cancer of Mice: Oxidative Stress and Other Biological Events |
title_sort | immunotherapy with monoclonal antibodies in lung cancer of mice oxidative stress and other biological events |
topic | experimental lung cancer immunomodulators oxidative stress autophagy tumor growth sirtuin-1 |
url | https://www.mdpi.com/2072-6694/11/9/1301 |
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