<i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’
In Germany, the phloem-sucking planthopper <i>Pentastiridius leporinus</i> (Hemiptera: Cixiidae) currently represents the epidemiological driver for the spread of the syndrome “Basses Richesses” in sugar beets, which results in a reduced sugar content and an economic loss for the farmers...
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MDPI AG
2024-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/3/189 |
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| author | Eva Therhaag Bernd Schneider Kerstin Zikeli Michael Maixner Jürgen Gross |
| author_facet | Eva Therhaag Bernd Schneider Kerstin Zikeli Michael Maixner Jürgen Gross |
| author_sort | Eva Therhaag |
| collection | DOAJ |
| description | In Germany, the phloem-sucking planthopper <i>Pentastiridius leporinus</i> (Hemiptera: Cixiidae) currently represents the epidemiological driver for the spread of the syndrome “Basses Richesses” in sugar beets, which results in a reduced sugar content and an economic loss for the farmers. This disease is associated with the γ-proteobacterium ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ and the Stolbur phytoplasma ‘<i>Candidatus</i> Phytoplasma solani’. Recently, <i>P. leporinus</i> was found in potato fields in Germany and is associated with Stolbur-like symptoms in this crop. In this study, we confirmed that the vector completes its lifecycle on sugar beets as well as on potatoes when reared under controlled conditions. Transmission experiments with adults of this vector combined with molecular analyses showed, for the first time, that both pathogens are transmitted by this vector to potatoes. For an accurate assessment of the Ca. P. solani and Ca. A. phytopathogenicus titers in the vector and host plants, gBlocks derived from the <i>hsp</i>20- and 16S rRNA genes were employed, respectively. For Ca. P. solani, the limit of detection was determined in potato and sugar beet material. The results of this study will further the research on the epidemiology of the syndrome “Basses Richesses” and “Stolbur” diseases and the response of host plants and vector insects to both pathogens. |
| first_indexed | 2024-04-24T18:10:12Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-04-24T18:10:12Z |
| publishDate | 2024-03-01 |
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| series | Insects |
| spelling | doaj.art-56d362e77dae47258fd9b11f066f55eb2024-03-27T13:47:17ZengMDPI AGInsects2075-44502024-03-0115318910.3390/insects15030189<i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’Eva Therhaag0Bernd Schneider1Kerstin Zikeli2Michael Maixner3Jürgen Gross4Institute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 69221 Dossenheim, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 69221 Dossenheim, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 69221 Dossenheim, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 76833 Siebeldingen, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 69221 Dossenheim, GermanyIn Germany, the phloem-sucking planthopper <i>Pentastiridius leporinus</i> (Hemiptera: Cixiidae) currently represents the epidemiological driver for the spread of the syndrome “Basses Richesses” in sugar beets, which results in a reduced sugar content and an economic loss for the farmers. This disease is associated with the γ-proteobacterium ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ and the Stolbur phytoplasma ‘<i>Candidatus</i> Phytoplasma solani’. Recently, <i>P. leporinus</i> was found in potato fields in Germany and is associated with Stolbur-like symptoms in this crop. In this study, we confirmed that the vector completes its lifecycle on sugar beets as well as on potatoes when reared under controlled conditions. Transmission experiments with adults of this vector combined with molecular analyses showed, for the first time, that both pathogens are transmitted by this vector to potatoes. For an accurate assessment of the Ca. P. solani and Ca. A. phytopathogenicus titers in the vector and host plants, gBlocks derived from the <i>hsp</i>20- and 16S rRNA genes were employed, respectively. For Ca. P. solani, the limit of detection was determined in potato and sugar beet material. The results of this study will further the research on the epidemiology of the syndrome “Basses Richesses” and “Stolbur” diseases and the response of host plants and vector insects to both pathogens.https://www.mdpi.com/2075-4450/15/3/189<i>Pentastiridius leporinus</i>potatoStolburproteobacteriumtransmissiongBlocks |
| spellingShingle | Eva Therhaag Bernd Schneider Kerstin Zikeli Michael Maixner Jürgen Gross <i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’ Insects <i>Pentastiridius leporinus</i> potato Stolbur proteobacterium transmission gBlocks |
| title | <i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’ |
| title_full | <i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’ |
| title_fullStr | <i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’ |
| title_full_unstemmed | <i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’ |
| title_short | <i>Pentastiridius leporinus</i> (Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ and ‘<i>Candidatus</i> Phytoplasma Solani’ |
| title_sort | i pentastiridius leporinus i linnaeus 1761 as a vector of phloem restricted pathogens on potatoes i candidatus i arsenophonus phytopathogenicus and i candidatus i phytoplasma solani |
| topic | <i>Pentastiridius leporinus</i> potato Stolbur proteobacterium transmission gBlocks |
| url | https://www.mdpi.com/2075-4450/15/3/189 |
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