Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems
Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report...
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MDPI AG
2021-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/18/3020 |
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| author | Bruno M. Guerreiro Jorge Carvalho Silva João Carlos Lima Maria A. M. Reis Filomena Freitas |
| author_facet | Bruno M. Guerreiro Jorge Carvalho Silva João Carlos Lima Maria A. M. Reis Filomena Freitas |
| author_sort | Bruno M. Guerreiro |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by <i>Enterobacter</i> A47, containing ca. 17 wt% of negatively charged residues in its structure. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe<sup>3+</sup>-TPTZ species at an EC<sub>50</sub> of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% <i>w</i>/<i>v</i> FucoPol concentration shown previously to be cryo- and photoprotective was also demonstrated to protect Vero cells against H<sub>2</sub>O<sub>2</sub>-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality. |
| first_indexed | 2024-03-10T07:17:20Z |
| format | Article |
| id | doaj.art-56d5f9e4ce84483985c0060c7fa83a0b |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T07:17:20Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-56d5f9e4ce84483985c0060c7fa83a0b2023-11-22T14:54:52ZengMDPI AGPolymers2073-43602021-09-011318302010.3390/polym13183020Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing SystemsBruno M. Guerreiro0Jorge Carvalho Silva1João Carlos Lima2Maria A. M. Reis3Filomena Freitas4Associate Laboratory i4HB—Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalCENIMAT/I3N, Department of Physics, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalLAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalReactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by <i>Enterobacter</i> A47, containing ca. 17 wt% of negatively charged residues in its structure. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe<sup>3+</sup>-TPTZ species at an EC<sub>50</sub> of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% <i>w</i>/<i>v</i> FucoPol concentration shown previously to be cryo- and photoprotective was also demonstrated to protect Vero cells against H<sub>2</sub>O<sub>2</sub>-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.https://www.mdpi.com/2073-4360/13/18/3020FucoPolpolysaccharideantioxidantstressFRAPHill |
| spellingShingle | Bruno M. Guerreiro Jorge Carvalho Silva João Carlos Lima Maria A. M. Reis Filomena Freitas Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems Polymers FucoPol polysaccharide antioxidant stress FRAP Hill |
| title | Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems |
| title_full | Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems |
| title_fullStr | Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems |
| title_full_unstemmed | Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems |
| title_short | Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems |
| title_sort | antioxidant potential of the bio based fucose rich polysaccharide fucopol supports its use in oxidative stress inducing systems |
| topic | FucoPol polysaccharide antioxidant stress FRAP Hill |
| url | https://www.mdpi.com/2073-4360/13/18/3020 |
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