Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings
Anthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resis...
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/15/2793 |
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| author | Jie Wang Zhipeng Yuan Delin Li Minghao Cai Zhi Liang Quanquan Chen Xuemei Du Jianhua Wang Riliang Gu Li Li |
| author_facet | Jie Wang Zhipeng Yuan Delin Li Minghao Cai Zhi Liang Quanquan Chen Xuemei Du Jianhua Wang Riliang Gu Li Li |
| author_sort | Jie Wang |
| collection | DOAJ |
| description | Anthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resistance via anthocyanin accumulation, and its potential molecular mechanism is still unclear. Therefore, it is of great significance for the normal growth and development of maize seedlings to explore the potential molecular mechanism of anthocyanin improving salt tolerance of seedlings via transcriptome analysis. In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions. In order to identify the specific genes involved in seedlings’ salt stress response, we generated two recombinant inbred lines (RIL<sup>pur–W22</sup> and RIL<sup>bro–W22</sup>) by crossing pur–W22 and bro–W22, and then performed transcriptome analysis on seedlings grown under both non–treatment and salt treatment conditions. A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RIL<sup>pur–W22</sup> and RIL<sup>bro–W22</sup> seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups. Among these DEGs, 3160 were identified as being present in both RIL<sup>pur–W22</sup> and RIL<sup>bro–W22</sup>, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation. Furthermore, we detected 1728 specific DEGs in the salt–tolerant RIL<sup>pur–W22</sup> line that were not detected in the salt–sensitive RIL<sup>bro–W22</sup> line, of which 887 were upregulated and 841 were downregulated. These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane. Notably, the anthocyanin synthesis related genes in RIL<sup>pur–W22</sup> were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings. In summary, the results of the transcriptome analysis not only expanded our understanding of the complex molecular mechanism of anthocyanin in improving the salt tolerance of maize seedlings, but also, the DEGs specifically expressed in the salt–tolerant line (RIL<sup>pur–W22</sup>) provided candidate genes for further genetic analysis. |
| first_indexed | 2024-03-11T00:19:28Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T00:19:28Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-56da977bbd3b41f4ad7fc4278b6eec392023-11-18T23:25:57ZengMDPI AGPlants2223-77472023-07-011215279310.3390/plants12152793Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize SeedlingsJie Wang0Zhipeng Yuan1Delin Li2Minghao Cai3Zhi Liang4Quanquan Chen5Xuemei Du6Jianhua Wang7Riliang Gu8Li Li9Beijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaBeijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaAnthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resistance via anthocyanin accumulation, and its potential molecular mechanism is still unclear. Therefore, it is of great significance for the normal growth and development of maize seedlings to explore the potential molecular mechanism of anthocyanin improving salt tolerance of seedlings via transcriptome analysis. In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions. In order to identify the specific genes involved in seedlings’ salt stress response, we generated two recombinant inbred lines (RIL<sup>pur–W22</sup> and RIL<sup>bro–W22</sup>) by crossing pur–W22 and bro–W22, and then performed transcriptome analysis on seedlings grown under both non–treatment and salt treatment conditions. A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RIL<sup>pur–W22</sup> and RIL<sup>bro–W22</sup> seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups. Among these DEGs, 3160 were identified as being present in both RIL<sup>pur–W22</sup> and RIL<sup>bro–W22</sup>, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation. Furthermore, we detected 1728 specific DEGs in the salt–tolerant RIL<sup>pur–W22</sup> line that were not detected in the salt–sensitive RIL<sup>bro–W22</sup> line, of which 887 were upregulated and 841 were downregulated. These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane. Notably, the anthocyanin synthesis related genes in RIL<sup>pur–W22</sup> were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings. In summary, the results of the transcriptome analysis not only expanded our understanding of the complex molecular mechanism of anthocyanin in improving the salt tolerance of maize seedlings, but also, the DEGs specifically expressed in the salt–tolerant line (RIL<sup>pur–W22</sup>) provided candidate genes for further genetic analysis.https://www.mdpi.com/2223-7747/12/15/2793maizeseedling growthsalt stress toleranceanthocyanidintranscriptome sequencing |
| spellingShingle | Jie Wang Zhipeng Yuan Delin Li Minghao Cai Zhi Liang Quanquan Chen Xuemei Du Jianhua Wang Riliang Gu Li Li Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings Plants maize seedling growth salt stress tolerance anthocyanidin transcriptome sequencing |
| title | Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings |
| title_full | Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings |
| title_fullStr | Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings |
| title_full_unstemmed | Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings |
| title_short | Transcriptome Analysis Revealed the Potential Molecular Mechanism of Anthocyanidins’ Improved Salt Tolerance in Maize Seedlings |
| title_sort | transcriptome analysis revealed the potential molecular mechanism of anthocyanidins improved salt tolerance in maize seedlings |
| topic | maize seedling growth salt stress tolerance anthocyanidin transcriptome sequencing |
| url | https://www.mdpi.com/2223-7747/12/15/2793 |
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