Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021
Abstract Background OXA-232-producing Klebsiella pneumoniae was first identified in China in 2016, and its clonal transmission was reported in 2019. However, there are no prevalence and genotypic surveillance data available for OXA-232 in China. Therefore, we investigated the trends and characterist...
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Infectious Diseases of Poverty |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40249-023-01051-w |
| _version_ | 1797827292066480128 |
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| author | Yanyan Zhang Xuemei Yang Congcong Liu Ling Huang Lingbin Shu Qiaoling Sun Hongwei Zhou Yonglu Huang Chang Cai Xiaoyan Wu Sheng Chen Rong Zhang |
| author_facet | Yanyan Zhang Xuemei Yang Congcong Liu Ling Huang Lingbin Shu Qiaoling Sun Hongwei Zhou Yonglu Huang Chang Cai Xiaoyan Wu Sheng Chen Rong Zhang |
| author_sort | Yanyan Zhang |
| collection | DOAJ |
| description | Abstract Background OXA-232-producing Klebsiella pneumoniae was first identified in China in 2016, and its clonal transmission was reported in 2019. However, there are no prevalence and genotypic surveillance data available for OXA-232 in China. Therefore, we investigated the trends and characteristics of OXA-232 type carbapenemase in Zhejiang Province, China from 2018 to 2021. Methods A total of 3278 samples from 1666 patients in the intensive care units were collected from hospitals in Zhejiang Province from 2018 to 2021. Carbapenem-resistant isolates were initially selected by China Blue agar plates supplemented with 0.3 μg/ml meropenem, and further analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry identification, immune colloidal gold technique, conjugation experiment, antimicrobial susceptibility testing and whole genome sequencing. Results A total of 79 OXA-producing strains were recovered, with the prevalence increased from 1.8% [95% confidence interval (CI): 0.7–3.7%] in 2018 to 6.0% (95% CI: 4.4–7.9%) in 2021. Seventy-eight strains produced OXA-232 and one produced OXA-181. The bla OXA-232 gene in all strains was located in a 6141-bp ColKP3-type non-conjugative plasmid and the bla OXA-181 gene was located in a 51,391-bp ColKP3/IncX3-type non-conjugative plasmid. The bla OXA-232-producing K. pneumoniae was dominated (75/76) by isolates of sequence type 15 (ST15) that differed by less than 80 SNPs. All OXA-producing strains (100%, 95% CI: 95.4–100.0%) were multidrug-resistant. Conclusions From 2018 to 2021, OXA-232 is the most prevalent OXA-48-like derivative in Zhejiang Province, and ST15 K. pneumoniae isolates belonging to the same clone are the major carriers. The transmission of ColKP3-type plasmid to E. coli highlighted that understanding the transmission mechanism is of great importance to delay or arrest the propagation of OXA-232 to other species. |
| first_indexed | 2024-04-09T12:46:07Z |
| format | Article |
| id | doaj.art-56db68625b364a12a0001762733b9a17 |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-04-09T12:46:07Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Diseases of Poverty |
| spelling | doaj.art-56db68625b364a12a0001762733b9a172023-05-14T11:31:26ZengBMCInfectious Diseases of Poverty2049-99572023-03-011211710.1186/s40249-023-01051-wIncreased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021Yanyan Zhang0Xuemei Yang1Congcong Liu2Ling Huang3Lingbin Shu4Qiaoling Sun5Hongwei Zhou6Yonglu Huang7Chang Cai8Xiaoyan Wu9Sheng Chen10Rong Zhang11Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong KongDepartment of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Clinical Laboratory Medicine, Maternal and Child Health Hospital of Yuhang DistrictDepartment of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineDepartment of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineCollege of Animal Science and Technology, Zhejiang Agricultural and Forestry UniversityThe Clinical Laboratory, Jiaxing Second HospitalDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong KongDepartment of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of MedicineAbstract Background OXA-232-producing Klebsiella pneumoniae was first identified in China in 2016, and its clonal transmission was reported in 2019. However, there are no prevalence and genotypic surveillance data available for OXA-232 in China. Therefore, we investigated the trends and characteristics of OXA-232 type carbapenemase in Zhejiang Province, China from 2018 to 2021. Methods A total of 3278 samples from 1666 patients in the intensive care units were collected from hospitals in Zhejiang Province from 2018 to 2021. Carbapenem-resistant isolates were initially selected by China Blue agar plates supplemented with 0.3 μg/ml meropenem, and further analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry identification, immune colloidal gold technique, conjugation experiment, antimicrobial susceptibility testing and whole genome sequencing. Results A total of 79 OXA-producing strains were recovered, with the prevalence increased from 1.8% [95% confidence interval (CI): 0.7–3.7%] in 2018 to 6.0% (95% CI: 4.4–7.9%) in 2021. Seventy-eight strains produced OXA-232 and one produced OXA-181. The bla OXA-232 gene in all strains was located in a 6141-bp ColKP3-type non-conjugative plasmid and the bla OXA-181 gene was located in a 51,391-bp ColKP3/IncX3-type non-conjugative plasmid. The bla OXA-232-producing K. pneumoniae was dominated (75/76) by isolates of sequence type 15 (ST15) that differed by less than 80 SNPs. All OXA-producing strains (100%, 95% CI: 95.4–100.0%) were multidrug-resistant. Conclusions From 2018 to 2021, OXA-232 is the most prevalent OXA-48-like derivative in Zhejiang Province, and ST15 K. pneumoniae isolates belonging to the same clone are the major carriers. The transmission of ColKP3-type plasmid to E. coli highlighted that understanding the transmission mechanism is of great importance to delay or arrest the propagation of OXA-232 to other species.https://doi.org/10.1186/s40249-023-01051-wKlebsiella pneumoniaeEscherichia coliOXA-232ColKP3Clonal dissemination |
| spellingShingle | Yanyan Zhang Xuemei Yang Congcong Liu Ling Huang Lingbin Shu Qiaoling Sun Hongwei Zhou Yonglu Huang Chang Cai Xiaoyan Wu Sheng Chen Rong Zhang Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021 Infectious Diseases of Poverty Klebsiella pneumoniae Escherichia coli OXA-232 ColKP3 Clonal dissemination |
| title | Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021 |
| title_full | Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021 |
| title_fullStr | Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021 |
| title_full_unstemmed | Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021 |
| title_short | Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021 |
| title_sort | increased clonal dissemination of oxa 232 producing st15 klebsiella pneumoniae in zhejiang china from 2018 to 2021 |
| topic | Klebsiella pneumoniae Escherichia coli OXA-232 ColKP3 Clonal dissemination |
| url | https://doi.org/10.1186/s40249-023-01051-w |
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