DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers
Abstract Aims Cigarette smoking is a preventable risk factor for various diseases such as cancer, ischemic stroke, cardiac stroke, and chronic obstructive pulmonary disease. Smoking cessation is of great importance not only for individual smokers but also for social health. Regarding current cessati...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Neuropsychopharmacology Reports |
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| Online Access: | https://doi.org/10.1002/npr2.12373 |
| _version_ | 1827576352729989120 |
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| author | Naotaka Izuo Hajime Miyanishi Daisuke Nishizawa Takuma Fujii Junko Hasegawa Naomi Sato Fumihiko Tanioka Haruhiko Sugimura Kazutaka Ikeda Atsumi Nitta |
| author_facet | Naotaka Izuo Hajime Miyanishi Daisuke Nishizawa Takuma Fujii Junko Hasegawa Naomi Sato Fumihiko Tanioka Haruhiko Sugimura Kazutaka Ikeda Atsumi Nitta |
| author_sort | Naotaka Izuo |
| collection | DOAJ |
| description | Abstract Aims Cigarette smoking is a preventable risk factor for various diseases such as cancer, ischemic stroke, cardiac stroke, and chronic obstructive pulmonary disease. Smoking cessation is of great importance not only for individual smokers but also for social health. Regarding current cessation therapies, the effectiveness of nicotine replacement is limited, and the cost of varenicline medication is considerable. Thus, a method for screening smokers who are responsive to cessation therapy based on the therapeutic effectiveness is required. Peripheral biomarkers reflecting smoking dependence status are necessary to establish a method for achieving effective cessation therapy. Methods Methylation status of smokers' blood DNA was evaluated focusing on SHATI/NAT8L, an addiction‐related gene. Eight CpG sites in SHATI/NAT8L were quantified by pyrosequencing. Results There was no difference in the methylation status of this gene between smokers (n = 129) and non‐smokers (n = 129) at all CpG sites. No correlations between the methylation status of SHATI/NAT8L and indicators of smoking dependence were found. Conclusions Although the present study found no significance in the DNA methylation of SHATI/NAT8L among smokers, the exploration of predictable peripheral biomarkers for the effectiveness of smoking cessation therapy is required. |
| first_indexed | 2024-03-08T21:10:19Z |
| format | Article |
| id | doaj.art-56dcf0fd166b4a778b918d1fa292bc06 |
| institution | Directory Open Access Journal |
| issn | 2574-173X |
| language | English |
| last_indexed | 2024-03-08T21:10:19Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neuropsychopharmacology Reports |
| spelling | doaj.art-56dcf0fd166b4a778b918d1fa292bc062023-12-22T07:25:34ZengWileyNeuropsychopharmacology Reports2574-173X2023-12-0143457057510.1002/npr2.12373DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokersNaotaka Izuo0Hajime Miyanishi1Daisuke Nishizawa2Takuma Fujii3Junko Hasegawa4Naomi Sato5Fumihiko Tanioka6Haruhiko Sugimura7Kazutaka Ikeda8Atsumi Nitta9Department of Pharmaceutical Therapy and Neuropharmacology, Faculty of Pharmaceutical Sciences University of Toyama Toyama JapanDepartment of Pharmaceutical Therapy and Neuropharmacology, Faculty of Pharmaceutical Sciences University of Toyama Toyama JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanDepartment of Pharmaceutical Therapy and Neuropharmacology, Faculty of Pharmaceutical Sciences University of Toyama Toyama JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanDepartment of Clinical Nursing Hamamatsu University School of Medicine Shizuoka JapanDepartment of Pathology Iwata City Hospital Shizuoka JapanDepartment of Tumor Pathology Hamamatsu University School of Medicine Shizuoka JapanAddictive Substance Project Tokyo Metropolitan Institute of Medical Science Tokyo JapanDepartment of Pharmaceutical Therapy and Neuropharmacology, Faculty of Pharmaceutical Sciences University of Toyama Toyama JapanAbstract Aims Cigarette smoking is a preventable risk factor for various diseases such as cancer, ischemic stroke, cardiac stroke, and chronic obstructive pulmonary disease. Smoking cessation is of great importance not only for individual smokers but also for social health. Regarding current cessation therapies, the effectiveness of nicotine replacement is limited, and the cost of varenicline medication is considerable. Thus, a method for screening smokers who are responsive to cessation therapy based on the therapeutic effectiveness is required. Peripheral biomarkers reflecting smoking dependence status are necessary to establish a method for achieving effective cessation therapy. Methods Methylation status of smokers' blood DNA was evaluated focusing on SHATI/NAT8L, an addiction‐related gene. Eight CpG sites in SHATI/NAT8L were quantified by pyrosequencing. Results There was no difference in the methylation status of this gene between smokers (n = 129) and non‐smokers (n = 129) at all CpG sites. No correlations between the methylation status of SHATI/NAT8L and indicators of smoking dependence were found. Conclusions Although the present study found no significance in the DNA methylation of SHATI/NAT8L among smokers, the exploration of predictable peripheral biomarkers for the effectiveness of smoking cessation therapy is required.https://doi.org/10.1002/npr2.12373cigarette smokerDNA methylationpyrosequencingSHATI/NAT8Ltobacco dependence screener |
| spellingShingle | Naotaka Izuo Hajime Miyanishi Daisuke Nishizawa Takuma Fujii Junko Hasegawa Naomi Sato Fumihiko Tanioka Haruhiko Sugimura Kazutaka Ikeda Atsumi Nitta DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers Neuropsychopharmacology Reports cigarette smoker DNA methylation pyrosequencing SHATI/NAT8L tobacco dependence screener |
| title | DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers |
| title_full | DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers |
| title_fullStr | DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers |
| title_full_unstemmed | DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers |
| title_short | DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers |
| title_sort | dna methylation status of shati nat8l promoter in the blood of cigarette smokers |
| topic | cigarette smoker DNA methylation pyrosequencing SHATI/NAT8L tobacco dependence screener |
| url | https://doi.org/10.1002/npr2.12373 |
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