Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2
Abstract Quiescence, a reversible state of cell-cycle arrest, is an important state during both normal development and cancer progression. For example, in glioblastoma (GBM) quiescent glioblastoma stem cells (GSCs) play an important role in re-establishing the tumour, leading to relapse. While most...
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| Format: | Article |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51340-z |
| _version_ | 1797276541114122240 |
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| author | Richard Zhiming Fu Oliver Cottrell Luisa Cutillo Andrew Rowntree Zsolt Zador Heiko Wurdak Nancy Papalopulu Elli Marinopoulou |
| author_facet | Richard Zhiming Fu Oliver Cottrell Luisa Cutillo Andrew Rowntree Zsolt Zador Heiko Wurdak Nancy Papalopulu Elli Marinopoulou |
| author_sort | Richard Zhiming Fu |
| collection | DOAJ |
| description | Abstract Quiescence, a reversible state of cell-cycle arrest, is an important state during both normal development and cancer progression. For example, in glioblastoma (GBM) quiescent glioblastoma stem cells (GSCs) play an important role in re-establishing the tumour, leading to relapse. While most studies have focused on identifying differentially expressed genes between proliferative and quiescent cells as potential drivers of this transition, recent studies have shown the importance of protein oscillations in controlling the exit from quiescence of neural stem cells. Here, we have undertaken a genome-wide bioinformatic inference approach to identify genes whose expression oscillates and which may be good candidates for controlling the transition to and from the quiescent cell state in GBM. Our analysis identified, among others, a list of important transcription regulators as potential oscillators, including the stemness gene SOX2, which we verified to oscillate in quiescent GSCs. These findings expand on the way we think about gene regulation and introduce new candidate genes as key regulators of quiescence. |
| first_indexed | 2024-03-07T15:30:36Z |
| format | Article |
| id | doaj.art-56dfffda789b46aeb5080b706c1a7b7b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:30:36Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
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| spelling | doaj.art-56dfffda789b46aeb5080b706c1a7b7b2024-03-05T16:28:01ZengNature PortfolioScientific Reports2045-23222024-01-0114111510.1038/s41598-024-51340-zIdentification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2Richard Zhiming Fu0Oliver Cottrell1Luisa Cutillo2Andrew Rowntree3Zsolt Zador4Heiko Wurdak5Nancy Papalopulu6Elli Marinopoulou7Division of Developmental Biology and Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterDivision of Developmental Biology and Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterSchool of Mathematics, University of LeedsDivision of Developmental Biology and Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterDivision of Neurosurgery, Department of Surgery, St. Michael’s HospitalStem Cell and Brain Tumour Group, Leeds Institute of Medical Research at St James’s, School of Medicine, University of LeedsDivision of Developmental Biology and Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterDivision of Developmental Biology and Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterAbstract Quiescence, a reversible state of cell-cycle arrest, is an important state during both normal development and cancer progression. For example, in glioblastoma (GBM) quiescent glioblastoma stem cells (GSCs) play an important role in re-establishing the tumour, leading to relapse. While most studies have focused on identifying differentially expressed genes between proliferative and quiescent cells as potential drivers of this transition, recent studies have shown the importance of protein oscillations in controlling the exit from quiescence of neural stem cells. Here, we have undertaken a genome-wide bioinformatic inference approach to identify genes whose expression oscillates and which may be good candidates for controlling the transition to and from the quiescent cell state in GBM. Our analysis identified, among others, a list of important transcription regulators as potential oscillators, including the stemness gene SOX2, which we verified to oscillate in quiescent GSCs. These findings expand on the way we think about gene regulation and introduce new candidate genes as key regulators of quiescence.https://doi.org/10.1038/s41598-024-51340-z |
| spellingShingle | Richard Zhiming Fu Oliver Cottrell Luisa Cutillo Andrew Rowntree Zsolt Zador Heiko Wurdak Nancy Papalopulu Elli Marinopoulou Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2 Scientific Reports |
| title | Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2 |
| title_full | Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2 |
| title_fullStr | Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2 |
| title_full_unstemmed | Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2 |
| title_short | Identification of genes with oscillatory expression in glioblastoma: the paradigm of SOX2 |
| title_sort | identification of genes with oscillatory expression in glioblastoma the paradigm of sox2 |
| url | https://doi.org/10.1038/s41598-024-51340-z |
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