Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films
To date, the need for biomaterials capable of improving the treatment of chronic skin wounds remains a clinical challenge. The aim of the present work is to formulate and characterize chitosan (Cs)/hydrolyzed collagen (HC) films as potential biomaterials with improved mechanical and hydration perfor...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/15/3/69 |
| _version_ | 1797240473970016256 |
|---|---|
| author | Tomás Martínez Rodríguez Caterina Valentino Francisco Ramón Rodríguez Pozo Pablo Hernández Benavides Francisco Arrebola Vargas José Manuel Paredes Claro Ignacio Sainz-Díaz Guillermo R. Iglesias Silvia Rossi Giuseppina Sandri María del Mar Medina Pérez Carola Aguzzi |
| author_facet | Tomás Martínez Rodríguez Caterina Valentino Francisco Ramón Rodríguez Pozo Pablo Hernández Benavides Francisco Arrebola Vargas José Manuel Paredes Claro Ignacio Sainz-Díaz Guillermo R. Iglesias Silvia Rossi Giuseppina Sandri María del Mar Medina Pérez Carola Aguzzi |
| author_sort | Tomás Martínez Rodríguez |
| collection | DOAJ |
| description | To date, the need for biomaterials capable of improving the treatment of chronic skin wounds remains a clinical challenge. The aim of the present work is to formulate and characterize chitosan (Cs)/hydrolyzed collagen (HC) films as potential biomaterials with improved mechanical and hydration performances compared to single component formulations. Films were made by the solvent casting method, with or without glycerin and/or PEG1500 as plasticizers, resulting in a total of eight formulations. All films were characterized by their physico-chemical characteristics and their mechanical and hydration features. A full factorial design was also used to statistically assess the effect of HC concentration, type and concentration of plasticizers and their possible interactions on mechanical and swelling behaviors. Solid state characterization confirmed the hybrid nature of the films, with suggested electrostatic interactions between Cs and HC. Mechanical and swelling properties, along with the analysis of the experimental design, allowed the identification of formulations containing high HC concentration (2% <i>w</i>/<i>v</i>) and glycerin or glycerin/PEG1500 as more suitable candidates for skin wound treatment. Finally, viability assay of immortalized human keratinocytes (HaCaT) showed no statistical differences in cell survival compared to the complete culture medium, suggesting their potential as a promising tool for biomedical applications. |
| first_indexed | 2024-04-24T18:08:00Z |
| format | Article |
| id | doaj.art-56e12b3376c64feaa451aa8f6acc72d0 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-24T18:08:00Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-56e12b3376c64feaa451aa8f6acc72d02024-03-27T13:48:39ZengMDPI AGJournal of Functional Biomaterials2079-49832024-03-011536910.3390/jfb15030069Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen FilmsTomás Martínez Rodríguez0Caterina Valentino1Francisco Ramón Rodríguez Pozo2Pablo Hernández Benavides3Francisco Arrebola Vargas4José Manuel Paredes5Claro Ignacio Sainz-Díaz6Guillermo R. Iglesias7Silvia Rossi8Giuseppina Sandri9María del Mar Medina Pérez10Carola Aguzzi11Department of Pharmacy and Pharmaceutical Technology, Cartuja Campus, University of Granada, 18071 Granada, SpainDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Pharmacy and Pharmaceutical Technology, Cartuja Campus, University of Granada, 18071 Granada, SpainDepartment of Pharmacy and Pharmaceutical Technology, Cartuja Campus, University of Granada, 18071 Granada, SpainDepartment of Histology, Institute of Neurosciences, Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainNanoscopy-UGR Laboratory, Department of Physical Chemistry, Unidad de Excelencia en Química Aplicada a Biomedicina y Medioambiente UEQ, Cartuja Campus, University of Granada, 18071 Granada, SpainInstituto Andaluz de Ciencias de la Tierra, CSIC-University of Granada, 18100 Armilla, SpainDepartment of Applied Physics, Faculty of Science, University of Granada, 18071 Granada, SpainDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Pharmacy and Pharmaceutical Technology, Cartuja Campus, University of Granada, 18071 Granada, SpainDepartment of Pharmacy and Pharmaceutical Technology, Cartuja Campus, University of Granada, 18071 Granada, SpainTo date, the need for biomaterials capable of improving the treatment of chronic skin wounds remains a clinical challenge. The aim of the present work is to formulate and characterize chitosan (Cs)/hydrolyzed collagen (HC) films as potential biomaterials with improved mechanical and hydration performances compared to single component formulations. Films were made by the solvent casting method, with or without glycerin and/or PEG1500 as plasticizers, resulting in a total of eight formulations. All films were characterized by their physico-chemical characteristics and their mechanical and hydration features. A full factorial design was also used to statistically assess the effect of HC concentration, type and concentration of plasticizers and their possible interactions on mechanical and swelling behaviors. Solid state characterization confirmed the hybrid nature of the films, with suggested electrostatic interactions between Cs and HC. Mechanical and swelling properties, along with the analysis of the experimental design, allowed the identification of formulations containing high HC concentration (2% <i>w</i>/<i>v</i>) and glycerin or glycerin/PEG1500 as more suitable candidates for skin wound treatment. Finally, viability assay of immortalized human keratinocytes (HaCaT) showed no statistical differences in cell survival compared to the complete culture medium, suggesting their potential as a promising tool for biomedical applications.https://www.mdpi.com/2079-4983/15/3/69chitosanhydrolyzed collagenfilmswound healingmechanical propertiesswelling |
| spellingShingle | Tomás Martínez Rodríguez Caterina Valentino Francisco Ramón Rodríguez Pozo Pablo Hernández Benavides Francisco Arrebola Vargas José Manuel Paredes Claro Ignacio Sainz-Díaz Guillermo R. Iglesias Silvia Rossi Giuseppina Sandri María del Mar Medina Pérez Carola Aguzzi Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films Journal of Functional Biomaterials chitosan hydrolyzed collagen films wound healing mechanical properties swelling |
| title | Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films |
| title_full | Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films |
| title_fullStr | Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films |
| title_full_unstemmed | Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films |
| title_short | Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films |
| title_sort | formulative study and characterization of novel biomaterials based on chitosan hydrolyzed collagen films |
| topic | chitosan hydrolyzed collagen films wound healing mechanical properties swelling |
| url | https://www.mdpi.com/2079-4983/15/3/69 |
| work_keys_str_mv | AT tomasmartinezrodriguez formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT caterinavalentino formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT franciscoramonrodriguezpozo formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT pablohernandezbenavides formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT franciscoarrebolavargas formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT josemanuelparedes formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT claroignaciosainzdiaz formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT guillermoriglesias formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT silviarossi formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT giuseppinasandri formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT mariadelmarmedinaperez formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms AT carolaaguzzi formulativestudyandcharacterizationofnovelbiomaterialsbasedonchitosanhydrolyzedcollagenfilms |