PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells
Protein tyrosine kinase 7 (PTK7), a catalytically defective receptor protein tyrosine kinase, is upregulated in tumor tissues and cell lines of esophageal squamous cell carcinoma (ESCC). We showed that PTK7 plays an oncogenic role in various ESCC cell lines. However, its role as an oncogene has not...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2391 |
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| author | Won-Sik Shin Mi-Kyung Park Jae Hoon Kim Si Won Oh Ji-Yun Jang Ho Lee Seung-Taek Lee |
| author_facet | Won-Sik Shin Mi-Kyung Park Jae Hoon Kim Si Won Oh Ji-Yun Jang Ho Lee Seung-Taek Lee |
| author_sort | Won-Sik Shin |
| collection | DOAJ |
| description | Protein tyrosine kinase 7 (PTK7), a catalytically defective receptor protein tyrosine kinase, is upregulated in tumor tissues and cell lines of esophageal squamous cell carcinoma (ESCC). We showed that PTK7 plays an oncogenic role in various ESCC cell lines. However, its role as an oncogene has not been demonstrated in vivo. Here, we examined the influence of PTK7 on the tumorigenic potential of ESCC KYSE-30 cells, which are known to establish xenograft tumors. Overexpression of PTK7 enhanced the proliferation, adhesion, wound healing, and migration of KYSE-30 cells, and these effects were reversed by the knockdown of <i>PTK7</i>. <i>PTK7</i> overexpression and knockdown, respectively, increased and decreased the tyrosine phosphorylation of cellular proteins and the phosphorylation of ERK, AKT, and FAK, which are important for cell proliferation, survival, adhesion, and migration. Additionally, <i>PTK7</i> overexpression and silencing, respectively, increased and decreased the weight, volume, and number of Ki-67-positive proliferating cells in xenograft tumors of KYSE-30 cells. Therefore, we propose that PTK7 plays an important role in the tumorigenesis of ESCC cells in vivo and is a potential therapeutic target for ESCC. |
| first_indexed | 2024-03-09T21:43:00Z |
| format | Article |
| id | doaj.art-56e148f53d934ffc9e22a38b534a5cf5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:43:00Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-56e148f53d934ffc9e22a38b534a5cf52023-11-23T20:24:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234239110.3390/ijms23042391PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 CellsWon-Sik Shin0Mi-Kyung Park1Jae Hoon Kim2Si Won Oh3Ji-Yun Jang4Ho Lee5Seung-Taek Lee6Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Cancer Biomedical Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang 10408, KoreaDepartment of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Cancer Biomedical Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang 10408, KoreaDepartment of Cancer Biomedical Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang 10408, KoreaDepartment of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, KoreaProtein tyrosine kinase 7 (PTK7), a catalytically defective receptor protein tyrosine kinase, is upregulated in tumor tissues and cell lines of esophageal squamous cell carcinoma (ESCC). We showed that PTK7 plays an oncogenic role in various ESCC cell lines. However, its role as an oncogene has not been demonstrated in vivo. Here, we examined the influence of PTK7 on the tumorigenic potential of ESCC KYSE-30 cells, which are known to establish xenograft tumors. Overexpression of PTK7 enhanced the proliferation, adhesion, wound healing, and migration of KYSE-30 cells, and these effects were reversed by the knockdown of <i>PTK7</i>. <i>PTK7</i> overexpression and knockdown, respectively, increased and decreased the tyrosine phosphorylation of cellular proteins and the phosphorylation of ERK, AKT, and FAK, which are important for cell proliferation, survival, adhesion, and migration. Additionally, <i>PTK7</i> overexpression and silencing, respectively, increased and decreased the weight, volume, and number of Ki-67-positive proliferating cells in xenograft tumors of KYSE-30 cells. Therefore, we propose that PTK7 plays an important role in the tumorigenesis of ESCC cells in vivo and is a potential therapeutic target for ESCC.https://www.mdpi.com/1422-0067/23/4/2391esophageal squamous cell carcinomaKYSE-30 cellsoncogenePTK7receptor protein tyrosine kinase |
| spellingShingle | Won-Sik Shin Mi-Kyung Park Jae Hoon Kim Si Won Oh Ji-Yun Jang Ho Lee Seung-Taek Lee PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells International Journal of Molecular Sciences esophageal squamous cell carcinoma KYSE-30 cells oncogene PTK7 receptor protein tyrosine kinase |
| title | PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells |
| title_full | PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells |
| title_fullStr | PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells |
| title_full_unstemmed | PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells |
| title_short | PTK7, a Catalytically Inactive Receptor Tyrosine Kinase, Increases Oncogenic Phenotypes in Xenograft Tumors of Esophageal Squamous Cell Carcinoma KYSE-30 Cells |
| title_sort | ptk7 a catalytically inactive receptor tyrosine kinase increases oncogenic phenotypes in xenograft tumors of esophageal squamous cell carcinoma kyse 30 cells |
| topic | esophageal squamous cell carcinoma KYSE-30 cells oncogene PTK7 receptor protein tyrosine kinase |
| url | https://www.mdpi.com/1422-0067/23/4/2391 |
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