Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer
Microcystins (MCs) are toxins produced by several cyanobacterial species found worldwide. While MCs have a common structure, the variation of two amino acids in their structure affects their toxicity. As toxicodynamics are very similar between the MC variants, their differential toxicity could rathe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/3/178 |
| _version_ | 1797417502476599296 |
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| author | Jérôme Henri Rachelle Lanceleur Jean-Michel Delmas Valérie Fessard Antoine Huguet |
| author_facet | Jérôme Henri Rachelle Lanceleur Jean-Michel Delmas Valérie Fessard Antoine Huguet |
| author_sort | Jérôme Henri |
| collection | DOAJ |
| description | Microcystins (MCs) are toxins produced by several cyanobacterial species found worldwide. While MCs have a common structure, the variation of two amino acids in their structure affects their toxicity. As toxicodynamics are very similar between the MC variants, their differential toxicity could rather be explained by toxicokinetic parameters. Microcystin-RR (MC-RR) is the second most abundant congener and induces toxicity through oral exposure. As intestinal permeability is a key parameter of oral toxicokinetics, the apparent permeability of MC-RR across a differentiated intestinal Caco-2 cell monolayer was investigated. We observed a rapid and large decrease of MC-RR levels in the donor compartment. However, irrespective of the loaded concentration and exposure time, the permeabilities were very low from apical to basolateral compartments (from 4 to 15 × 10<sup>−8</sup> cm·s<sup>−1</sup>) and from basolateral to apical compartments (from 2 to 37 × 10<sup>−8</sup> cm·s<sup>−1</sup>). Our results suggested that MC-RR would be poorly absorbed orally. As similar low permeability was reported for the most abundant congener microcystin-LR, and this variant presented a greater acute oral toxicity than MC-RR, we concluded that the intestinal permeability was probably not involved in the differential toxicity between them, in contrast to the hepatic uptake and metabolism. |
| first_indexed | 2024-03-09T06:19:41Z |
| format | Article |
| id | doaj.art-56e1a9cc2e9d421c9b0965e8ef5557fd |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T06:19:41Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-56e1a9cc2e9d421c9b0965e8ef5557fd2023-12-03T11:49:09ZengMDPI AGToxins2072-66512021-02-0113317810.3390/toxins13030178Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells MonolayerJérôme Henri0Rachelle Lanceleur1Jean-Michel Delmas2Valérie Fessard3Antoine Huguet4Fougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, FranceFougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, FranceFougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, FranceFougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, FranceFougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, FranceMicrocystins (MCs) are toxins produced by several cyanobacterial species found worldwide. While MCs have a common structure, the variation of two amino acids in their structure affects their toxicity. As toxicodynamics are very similar between the MC variants, their differential toxicity could rather be explained by toxicokinetic parameters. Microcystin-RR (MC-RR) is the second most abundant congener and induces toxicity through oral exposure. As intestinal permeability is a key parameter of oral toxicokinetics, the apparent permeability of MC-RR across a differentiated intestinal Caco-2 cell monolayer was investigated. We observed a rapid and large decrease of MC-RR levels in the donor compartment. However, irrespective of the loaded concentration and exposure time, the permeabilities were very low from apical to basolateral compartments (from 4 to 15 × 10<sup>−8</sup> cm·s<sup>−1</sup>) and from basolateral to apical compartments (from 2 to 37 × 10<sup>−8</sup> cm·s<sup>−1</sup>). Our results suggested that MC-RR would be poorly absorbed orally. As similar low permeability was reported for the most abundant congener microcystin-LR, and this variant presented a greater acute oral toxicity than MC-RR, we concluded that the intestinal permeability was probably not involved in the differential toxicity between them, in contrast to the hepatic uptake and metabolism.https://www.mdpi.com/2072-6651/13/3/178microcystin-RRCaco-2 cellsintestinal permeability |
| spellingShingle | Jérôme Henri Rachelle Lanceleur Jean-Michel Delmas Valérie Fessard Antoine Huguet Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer Toxins microcystin-RR Caco-2 cells intestinal permeability |
| title | Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer |
| title_full | Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer |
| title_fullStr | Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer |
| title_full_unstemmed | Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer |
| title_short | Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer |
| title_sort | permeability of the cyanotoxin microcystin rr across a caco 2 cells monolayer |
| topic | microcystin-RR Caco-2 cells intestinal permeability |
| url | https://www.mdpi.com/2072-6651/13/3/178 |
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