Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2)
Modifiers of membranes cause local defects on the cell surface. Measurement of the rigidity at the sites of local defects can provide further information about the structure of defects and mechanical properties of altered membranes.The purpose of the study: a step-by-step study of the process of a n...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2018-03-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1636 |
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| author | Alexander M. Chernysh Elena K. Kozlova Viktor V. Moroz Viktoria A. Sergunova Olga E. Gudkova Alexander P. Kozlov Ekaterina A. Manchenko |
| author_facet | Alexander M. Chernysh Elena K. Kozlova Viktor V. Moroz Viktoria A. Sergunova Olga E. Gudkova Alexander P. Kozlov Ekaterina A. Manchenko |
| author_sort | Alexander M. Chernysh |
| collection | DOAJ |
| description | Modifiers of membranes cause local defects on the cell surface. Measurement of the rigidity at the sites of local defects can provide further information about the structure of defects and mechanical properties of altered membranes.The purpose of the study: a step-by-step study of the process of a nonlinear deformation of red blood cells membranes under the effect of modifiers of different physico-chemical nature.Materials and methods. The membrane deformation of a viscoelastic composite erythrocyte construction inside a cell was studied by the atomic force spectroscopy. Nonlinear deformations formed under the effect of hemin, Zn2+ ions, and verapamil were studied.Results. The process of elastic deformation of the membrane with the indentation of a probe at the sites of local defects caused by modifiers was demonstrated. The probe was inserted during the same step of the piezo scanner z displacement; the probe indentation occured at the different discrete values of h, which are the functions of the membrane structure. At the sites of domains, under the effect of the hemin, tension areas and plasticity areas appeared. A mathematical model of probe indentation at the site of membrane defects is presented.Conclusion. The molecular mechanisms of various types of nonlinear deformations occurring under the effect of toxins are discussed. The results of the study may be of interest both for fundamental researchers of the blood cell properties and for practical reanimatology and rehabilitology. |
| first_indexed | 2024-04-10T01:28:10Z |
| format | Article |
| id | doaj.art-56e3603521774ca584563c9e159e0c61 |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2025-03-14T09:44:47Z |
| publishDate | 2018-03-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-56e3603521774ca584563c9e159e0c612025-03-02T11:29:36ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102018-03-01141293910.15360/1813-9779-2018-1-29-391595Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2)Alexander M. Chernysh0Elena K. Kozlova1Viktor V. Moroz2Viktoria A. Sergunova3Olga E. Gudkova4Alexander P. Kozlov5Ekaterina A. Manchenko6V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology; I. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology; I. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyI. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology; I. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaModifiers of membranes cause local defects on the cell surface. Measurement of the rigidity at the sites of local defects can provide further information about the structure of defects and mechanical properties of altered membranes.The purpose of the study: a step-by-step study of the process of a nonlinear deformation of red blood cells membranes under the effect of modifiers of different physico-chemical nature.Materials and methods. The membrane deformation of a viscoelastic composite erythrocyte construction inside a cell was studied by the atomic force spectroscopy. Nonlinear deformations formed under the effect of hemin, Zn2+ ions, and verapamil were studied.Results. The process of elastic deformation of the membrane with the indentation of a probe at the sites of local defects caused by modifiers was demonstrated. The probe was inserted during the same step of the piezo scanner z displacement; the probe indentation occured at the different discrete values of h, which are the functions of the membrane structure. At the sites of domains, under the effect of the hemin, tension areas and plasticity areas appeared. A mathematical model of probe indentation at the site of membrane defects is presented.Conclusion. The molecular mechanisms of various types of nonlinear deformations occurring under the effect of toxins are discussed. The results of the study may be of interest both for fundamental researchers of the blood cell properties and for practical reanimatology and rehabilitology.https://www.reanimatology.com/rmt/article/view/1636red blood cells membranesnonlinear deformationsatomic force spectroscopy |
| spellingShingle | Alexander M. Chernysh Elena K. Kozlova Viktor V. Moroz Viktoria A. Sergunova Olga E. Gudkova Alexander P. Kozlov Ekaterina A. Manchenko Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2) Общая реаниматология red blood cells membranes nonlinear deformations atomic force spectroscopy |
| title | Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2) |
| title_full | Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2) |
| title_fullStr | Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2) |
| title_full_unstemmed | Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2) |
| title_short | Nonlinear Local Deformations of Red Blood Cell Membranes: Effects of Toxins and Pharmaceuticals (Part 2) |
| title_sort | nonlinear local deformations of red blood cell membranes effects of toxins and pharmaceuticals part 2 |
| topic | red blood cells membranes nonlinear deformations atomic force spectroscopy |
| url | https://www.reanimatology.com/rmt/article/view/1636 |
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