Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells
Periods of low energy supply are challenging conditions for organisms and cells during fasting or famine. Although changes in nutrient levels in the blood are first sensed by endothelial cells, studies on their metabolic adaptations to diminished energy supply are lacking. We analyzed the dynamic me...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1189 |
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| author | Mario Lorenz Raphaela Fritsche-Guenther Cornelia Bartsch Angelika Vietzke Alina Eisenberger Karl Stangl Verena Stangl Jennifer A. Kirwan |
| author_facet | Mario Lorenz Raphaela Fritsche-Guenther Cornelia Bartsch Angelika Vietzke Alina Eisenberger Karl Stangl Verena Stangl Jennifer A. Kirwan |
| author_sort | Mario Lorenz |
| collection | DOAJ |
| description | Periods of low energy supply are challenging conditions for organisms and cells during fasting or famine. Although changes in nutrient levels in the blood are first sensed by endothelial cells, studies on their metabolic adaptations to diminished energy supply are lacking. We analyzed the dynamic metabolic activity of human umbilical vein endothelial cells (HUVECs) in basal conditions and after serum starvation. Metabolites of glycolysis, the tricarboxylic acid (TCA) cycle, and the glycerol pathway showed lower levels after serum starvation, whereas amino acids had increased levels. A metabolic flux analysis with <sup>13</sup>C-glucose or <sup>13</sup>C-glutamine labeling for different time points reached a plateau phase of incorporation after 30 h for <sup>13</sup>C-glucose and after 8 h for <sup>13</sup>C-glutamine under both experimental conditions. Notably, we observed a faster label incorporation for both <sup>13</sup>C-glucose and <sup>13</sup>C-glutamine after serum starvation. In the linear range of label incorporation after 3 h, we found a significantly faster incorporation of central carbon metabolites after serum starvation compared to the basal state. These findings may indicate that endothelial cells develop increased metabolic activity to cope with energy deficiency. Physiologically, it can be a prerequisite for endothelial cells to form new blood vessels under unfavorable conditions during the process of angiogenesis in vivo. |
| first_indexed | 2024-03-09T12:24:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:24:44Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-56e7c98fb29149e7bc6f3159984a14f02023-11-30T22:36:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242118910.3390/ijms24021189Serum Starvation Accelerates Intracellular Metabolism in Endothelial CellsMario Lorenz0Raphaela Fritsche-Guenther1Cornelia Bartsch2Angelika Vietzke3Alina Eisenberger4Karl Stangl5Verena Stangl6Jennifer A. Kirwan7Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, 10117 Berlin, GermanyMetabolomics Platform, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, 10117 Berlin, GermanyCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, 10117 Berlin, GermanyCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, 10117 Berlin, GermanyMetabolomics Platform, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, 10117 Berlin, GermanyCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, 10117 Berlin, GermanyCharité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, 10117 Berlin, GermanyMetabolomics Platform, Berlin Institute of Health at Charité—Universitätsmedizin Berlin, 10117 Berlin, GermanyPeriods of low energy supply are challenging conditions for organisms and cells during fasting or famine. Although changes in nutrient levels in the blood are first sensed by endothelial cells, studies on their metabolic adaptations to diminished energy supply are lacking. We analyzed the dynamic metabolic activity of human umbilical vein endothelial cells (HUVECs) in basal conditions and after serum starvation. Metabolites of glycolysis, the tricarboxylic acid (TCA) cycle, and the glycerol pathway showed lower levels after serum starvation, whereas amino acids had increased levels. A metabolic flux analysis with <sup>13</sup>C-glucose or <sup>13</sup>C-glutamine labeling for different time points reached a plateau phase of incorporation after 30 h for <sup>13</sup>C-glucose and after 8 h for <sup>13</sup>C-glutamine under both experimental conditions. Notably, we observed a faster label incorporation for both <sup>13</sup>C-glucose and <sup>13</sup>C-glutamine after serum starvation. In the linear range of label incorporation after 3 h, we found a significantly faster incorporation of central carbon metabolites after serum starvation compared to the basal state. These findings may indicate that endothelial cells develop increased metabolic activity to cope with energy deficiency. Physiologically, it can be a prerequisite for endothelial cells to form new blood vessels under unfavorable conditions during the process of angiogenesis in vivo.https://www.mdpi.com/1422-0067/24/2/1189metabolismserum starvationendothelial cellsHUVECflux analysislabeling |
| spellingShingle | Mario Lorenz Raphaela Fritsche-Guenther Cornelia Bartsch Angelika Vietzke Alina Eisenberger Karl Stangl Verena Stangl Jennifer A. Kirwan Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells International Journal of Molecular Sciences metabolism serum starvation endothelial cells HUVEC flux analysis labeling |
| title | Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells |
| title_full | Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells |
| title_fullStr | Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells |
| title_full_unstemmed | Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells |
| title_short | Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells |
| title_sort | serum starvation accelerates intracellular metabolism in endothelial cells |
| topic | metabolism serum starvation endothelial cells HUVEC flux analysis labeling |
| url | https://www.mdpi.com/1422-0067/24/2/1189 |
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