Fast in vitro protocol for the visualization and quantitative high-throughput analysis of sprouting angiogenesis by confocal microscopy

Fast in vitro protocol for the visualization and quantitative high-throughput analysis of sprouting angiogenesis by confocal microscopy

Summary: We describe an optimized, cost-effective, reproducible, and robust protocol to study sprouting angiogenesis in glass-bottom 96-well plates by confocal microscopy, ideal for screening of drug or shRNA libraries. Effective and stable knockdown of gene expression in primary endothelial cells i...

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Bibliographic Details
Main Authors: Lanette Kempers, Ivo van der Bijl, Anne-Marieke D. van Stalborch, Bas Ponsioen, Coert Margadant
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell-based Assays
Developmental biology
High Throughput Screening
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100397X

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