Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction
Shuo Wang, Yuying Liu, Xitian Hu, Xiaolei Zhang, Lei Xu, Yan Yang, Rubing Wu, Enmao Wang, Tianjie Lv Department of Cardiovasology, Shijiazhuang People’s Hospital, Shijiazhuang, People’s Republic of ChinaCorrespondence: Xitian HuDepartment of Cardiovasology, Shijiazhuang People’s Hospital, No. 9 Fang...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-12-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/identification-of-cerna-lncrna-mirna-mrna-regulatory-network-in-myocar-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1819177629021896704 |
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| author | Wang S Liu Y Hu X Zhang X Xu L Yang Y Wu R Wang E Lv T |
| author_facet | Wang S Liu Y Hu X Zhang X Xu L Yang Y Wu R Wang E Lv T |
| author_sort | Wang S |
| collection | DOAJ |
| description | Shuo Wang, Yuying Liu, Xitian Hu, Xiaolei Zhang, Lei Xu, Yan Yang, Rubing Wu, Enmao Wang, Tianjie Lv Department of Cardiovasology, Shijiazhuang People’s Hospital, Shijiazhuang, People’s Republic of ChinaCorrespondence: Xitian HuDepartment of Cardiovasology, Shijiazhuang People’s Hospital, No. 9 Fangbei Road, Shijiazhuang, Hebei, 050000, People’s Republic of ChinaTel +86-17603119015Email huxitian007@163.comPurpose: Myocardial fibrosis (MF) after acute myocardial infarction (AMI) ultimately results in heart failure, which is a serious threat to human life. This study aimed to excavate critical biomarkers associated with MF after AMI.Materials and Methods: RNA-sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in AMI and MF after AMI.Results: Abundant DEmRNAs, DEmiRNAs and DElncRNAs were identified in AMI and MF after AMI. The ceRNA network, which contained 9 lncRNA-miRNA pairs and 9 miRNA-mRNA pairs, was acquired. In AMI, all candidate markers generally exhibited the same pattern as that in our RNA-seq results; while in MF after AMI, except for CENPB, JAK2 and hsa-miR-197-3p, the expression of the others in the qRT-PCR results exhibited the same pattern as that in our RNA-seq results.Conclusion: We speculated that LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs may serve as potential biomarkers in MF after AMI.Keywords: acute myocardial infarction, myocardial fibrosis, lncRNA, ceRNA network |
| first_indexed | 2024-12-22T21:29:41Z |
| format | Article |
| id | doaj.art-56ebc7c97cab4a04a88e093543df75d2 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-12-22T21:29:41Z |
| publishDate | 2021-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-56ebc7c97cab4a04a88e093543df75d22022-12-21T18:11:58ZengDove Medical PressInternational Journal of General Medicine1178-70742021-12-01Volume 149977999071630Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial InfarctionWang SLiu YHu XZhang XXu LYang YWu RWang ELv TShuo Wang, Yuying Liu, Xitian Hu, Xiaolei Zhang, Lei Xu, Yan Yang, Rubing Wu, Enmao Wang, Tianjie Lv Department of Cardiovasology, Shijiazhuang People’s Hospital, Shijiazhuang, People’s Republic of ChinaCorrespondence: Xitian HuDepartment of Cardiovasology, Shijiazhuang People’s Hospital, No. 9 Fangbei Road, Shijiazhuang, Hebei, 050000, People’s Republic of ChinaTel +86-17603119015Email huxitian007@163.comPurpose: Myocardial fibrosis (MF) after acute myocardial infarction (AMI) ultimately results in heart failure, which is a serious threat to human life. This study aimed to excavate critical biomarkers associated with MF after AMI.Materials and Methods: RNA-sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in AMI and MF after AMI.Results: Abundant DEmRNAs, DEmiRNAs and DElncRNAs were identified in AMI and MF after AMI. The ceRNA network, which contained 9 lncRNA-miRNA pairs and 9 miRNA-mRNA pairs, was acquired. In AMI, all candidate markers generally exhibited the same pattern as that in our RNA-seq results; while in MF after AMI, except for CENPB, JAK2 and hsa-miR-197-3p, the expression of the others in the qRT-PCR results exhibited the same pattern as that in our RNA-seq results.Conclusion: We speculated that LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs may serve as potential biomarkers in MF after AMI.Keywords: acute myocardial infarction, myocardial fibrosis, lncRNA, ceRNA networkhttps://www.dovepress.com/identification-of-cerna-lncrna-mirna-mrna-regulatory-network-in-myocar-peer-reviewed-fulltext-article-IJGMacute myocardial infarctionmyocardial fibrosislncrnacerna network |
| spellingShingle | Wang S Liu Y Hu X Zhang X Xu L Yang Y Wu R Wang E Lv T Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction International Journal of General Medicine acute myocardial infarction myocardial fibrosis lncrna cerna network |
| title | Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction |
| title_full | Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction |
| title_fullStr | Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction |
| title_full_unstemmed | Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction |
| title_short | Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction |
| title_sort | identification of cerna lncrna mirna mrna regulatory network in myocardial fibrosis after acute myocardial infarction |
| topic | acute myocardial infarction myocardial fibrosis lncrna cerna network |
| url | https://www.dovepress.com/identification-of-cerna-lncrna-mirna-mrna-regulatory-network-in-myocar-peer-reviewed-fulltext-article-IJGM |
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