The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms
The aim of the study was to determine the still not completely described microbiome associated with the aquatic fern <i>Azolla filiculoides</i>. During the experiment, 58 microbial isolates (43 epiphytes and 15 endophytes) with different morphologies were obtained. We successfully identi...
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MDPI AG
2019-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/10/2143 |
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| author | Artur Banach Agnieszka Kuźniar Radosław Mencfel Agnieszka Wolińska |
| author_facet | Artur Banach Agnieszka Kuźniar Radosław Mencfel Agnieszka Wolińska |
| author_sort | Artur Banach |
| collection | DOAJ |
| description | The aim of the study was to determine the still not completely described microbiome associated with the aquatic fern <i>Azolla filiculoides</i>. During the experiment, 58 microbial isolates (43 epiphytes and 15 endophytes) with different morphologies were obtained. We successfully identified 85% of microorganisms and assigned them to 9 bacterial genera: <i>Achromobacter</i>, <i>Bacillus</i>, <i>Microbacterium</i>, <i>Delftia</i>, <i>Agrobacterium</i>, and <i>Alcaligenes</i> (epiphytes) as well as <i>Bacillus</i>, <i>Staphylococcus</i>, <i>Micrococcus</i>, and <i>Acinetobacter</i> (endophytes). We also studied an <i>A. filiculoides</i> cyanobiont originally classified as <i>Anabaena azollae</i>; however, the analysis of its morphological traits suggests that this should be renamed as <i>Trichormus azollae</i>. Finally, the potential of the representatives of the identified microbial genera to synthesize plant growth-promoting substances such as indole-3-acetic acid (IAA), cellulase and protease enzymes, siderophores and phosphorus (P) and their potential of utilization thereof were checked. <i>Delftia</i> sp. AzoEpi7 was the only one from all the identified genera exhibiting the ability to synthesize all the studied growth promoters; thus, it was recommended as the most beneficial bacteria in the studied microbiome. The other three potentially advantageous isolates (<i>Micrococcus</i> sp. AzoEndo14, <i>Agrobacterium</i> sp. AzoEpi25 and <i>Bacillus</i> sp. AzoEndo3) displayed 5 parameters: IAA (excluding <i>Bacillus</i> sp. AzoEndo3), cellulase, protease, siderophores (excluding <i>Micrococcus</i> sp. AzoEndo14), as well as mineralization and solubilization of P (excluding <i>Agrobacterium</i> sp. AzoEpi25). |
| first_indexed | 2024-12-20T22:15:36Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-20T22:15:36Z |
| publishDate | 2019-05-01 |
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| spelling | doaj.art-56ee74c3635e4be3b4e3d9657bde83a22022-12-21T19:25:03ZengMDPI AGApplied Sciences2076-34172019-05-01910214310.3390/app9102143app9102143The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial MicroorganismsArtur Banach0Agnieszka Kuźniar1Radosław Mencfel2Agnieszka Wolińska3Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, 20-708 Lublin, PolandDepartment of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, 20-708 Lublin, PolandDepartment of Animal Physiology and Toxicology, The John Paul II Catholic University of Lublin, 20-708 Lublin, PolandDepartment of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, 20-708 Lublin, PolandThe aim of the study was to determine the still not completely described microbiome associated with the aquatic fern <i>Azolla filiculoides</i>. During the experiment, 58 microbial isolates (43 epiphytes and 15 endophytes) with different morphologies were obtained. We successfully identified 85% of microorganisms and assigned them to 9 bacterial genera: <i>Achromobacter</i>, <i>Bacillus</i>, <i>Microbacterium</i>, <i>Delftia</i>, <i>Agrobacterium</i>, and <i>Alcaligenes</i> (epiphytes) as well as <i>Bacillus</i>, <i>Staphylococcus</i>, <i>Micrococcus</i>, and <i>Acinetobacter</i> (endophytes). We also studied an <i>A. filiculoides</i> cyanobiont originally classified as <i>Anabaena azollae</i>; however, the analysis of its morphological traits suggests that this should be renamed as <i>Trichormus azollae</i>. Finally, the potential of the representatives of the identified microbial genera to synthesize plant growth-promoting substances such as indole-3-acetic acid (IAA), cellulase and protease enzymes, siderophores and phosphorus (P) and their potential of utilization thereof were checked. <i>Delftia</i> sp. AzoEpi7 was the only one from all the identified genera exhibiting the ability to synthesize all the studied growth promoters; thus, it was recommended as the most beneficial bacteria in the studied microbiome. The other three potentially advantageous isolates (<i>Micrococcus</i> sp. AzoEndo14, <i>Agrobacterium</i> sp. AzoEpi25 and <i>Bacillus</i> sp. AzoEndo3) displayed 5 parameters: IAA (excluding <i>Bacillus</i> sp. AzoEndo3), cellulase, protease, siderophores (excluding <i>Micrococcus</i> sp. AzoEndo14), as well as mineralization and solubilization of P (excluding <i>Agrobacterium</i> sp. AzoEpi25).https://www.mdpi.com/2076-3417/9/10/2143<i>Azolla</i><i>Delftia</i> sp., endophytesplant growth promoting potential<i>Trichormus azollae</i> |
| spellingShingle | Artur Banach Agnieszka Kuźniar Radosław Mencfel Agnieszka Wolińska The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms Applied Sciences <i>Azolla</i> <i>Delftia</i> sp., endophytes plant growth promoting potential <i>Trichormus azollae</i> |
| title | The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms |
| title_full | The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms |
| title_fullStr | The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms |
| title_full_unstemmed | The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms |
| title_short | The Study on the Cultivable Microbiome of the Aquatic Fern <i>Azolla Filiculoides</i> L. as New Source of Beneficial Microorganisms |
| title_sort | study on the cultivable microbiome of the aquatic fern i azolla filiculoides i l as new source of beneficial microorganisms |
| topic | <i>Azolla</i> <i>Delftia</i> sp., endophytes plant growth promoting potential <i>Trichormus azollae</i> |
| url | https://www.mdpi.com/2076-3417/9/10/2143 |
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