Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i>
Microorganisms can affect host reproduction, defense, and immunity through sexual or opportunistic transmission; however, there are few studies on insect reproductive organs and intestinal bacterial communities and their effects on mating. <i>Tuta absoluta</i> is a worldwide quarantine p...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/10/779 |
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| author | Siyan Bi Xiaodi Wang Yanhong Tang Kexin Lei Jianyang Guo Nianwan Yang Fanghao Wan Zhichuang Lü Wanxue Liu |
| author_facet | Siyan Bi Xiaodi Wang Yanhong Tang Kexin Lei Jianyang Guo Nianwan Yang Fanghao Wan Zhichuang Lü Wanxue Liu |
| author_sort | Siyan Bi |
| collection | DOAJ |
| description | Microorganisms can affect host reproduction, defense, and immunity through sexual or opportunistic transmission; however, there are few studies on insect reproductive organs and intestinal bacterial communities and their effects on mating. <i>Tuta absoluta</i> is a worldwide quarantine pest that seriously threatens the production of Solanaceae crops, and the microbial community within tomato leafminers remains unclear. In this study, 16s rRNA sequencing was used to analyze bacterial communities related to the reproductive organs and intestinal tracts of tomato leafminers (the sample accession numbers are from CNS0856533 to CNS0856577). Different bacterial communities were found in the reproductive organs and intestinal tracts of females and males. Community ecological analysis revealed three potential signs of bacterial sexual transmission: (1) Mating increased the similarity between male and female sex organs and intestinal communities. (2) The bacteria carried by mated individuals were found in unmated individuals of the opposite sex but not in unmated individuals of the same sex. (3) The bacteria carried by unmated individuals were lost after mating. In addition, the abundances of bacterial communities carried by eggs were significantly higher than those of adult worms. Our results confirm that mating leads to the transfer of bacterial communities in the reproductive organs and gut of tomato leafminers, and suggest that this community strongly influences the reproductive process. |
| first_indexed | 2024-03-10T21:10:43Z |
| format | Article |
| id | doaj.art-56ef7882a07f43a38dd619d12416bd3b |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T21:10:43Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-56ef7882a07f43a38dd619d12416bd3b2023-11-19T16:49:26ZengMDPI AGInsects2075-44502023-09-01141077910.3390/insects14100779Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i>Siyan Bi0Xiaodi Wang1Yanhong Tang2Kexin Lei3Jianyang Guo4Nianwan Yang5Fanghao Wan6Zhichuang Lü7Wanxue Liu8State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaMicroorganisms can affect host reproduction, defense, and immunity through sexual or opportunistic transmission; however, there are few studies on insect reproductive organs and intestinal bacterial communities and their effects on mating. <i>Tuta absoluta</i> is a worldwide quarantine pest that seriously threatens the production of Solanaceae crops, and the microbial community within tomato leafminers remains unclear. In this study, 16s rRNA sequencing was used to analyze bacterial communities related to the reproductive organs and intestinal tracts of tomato leafminers (the sample accession numbers are from CNS0856533 to CNS0856577). Different bacterial communities were found in the reproductive organs and intestinal tracts of females and males. Community ecological analysis revealed three potential signs of bacterial sexual transmission: (1) Mating increased the similarity between male and female sex organs and intestinal communities. (2) The bacteria carried by mated individuals were found in unmated individuals of the opposite sex but not in unmated individuals of the same sex. (3) The bacteria carried by unmated individuals were lost after mating. In addition, the abundances of bacterial communities carried by eggs were significantly higher than those of adult worms. Our results confirm that mating leads to the transfer of bacterial communities in the reproductive organs and gut of tomato leafminers, and suggest that this community strongly influences the reproductive process.https://www.mdpi.com/2075-4450/14/10/779matedmicrobial transmissionreproductive tracts<i>Tuta absoluta</i>16S rRNA |
| spellingShingle | Siyan Bi Xiaodi Wang Yanhong Tang Kexin Lei Jianyang Guo Nianwan Yang Fanghao Wan Zhichuang Lü Wanxue Liu Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i> Insects mated microbial transmission reproductive tracts <i>Tuta absoluta</i> 16S rRNA |
| title | Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i> |
| title_full | Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i> |
| title_fullStr | Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i> |
| title_full_unstemmed | Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i> |
| title_short | Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated <i>Tuta absoluta</i> |
| title_sort | bacterial communities of the internal reproductive and digestive tracts of virgin and mated i tuta absoluta i |
| topic | mated microbial transmission reproductive tracts <i>Tuta absoluta</i> 16S rRNA |
| url | https://www.mdpi.com/2075-4450/14/10/779 |
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