Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing
In the present study, a functional template made up of in situ synthesised gold nanoparticles (AuNPs) is prepared on polydimethylsiloxane (PDMS) for patterning of target protein onto the desired solid substrates. Unlike previous studies in which bioreceptor probes are randomly attached to the PDMS s...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/12/1067 |
| _version_ | 1797461208873304064 |
|---|---|
| author | Amna Didar Abbasi Zakir Hussain Kun-Lin Yang |
| author_facet | Amna Didar Abbasi Zakir Hussain Kun-Lin Yang |
| author_sort | Amna Didar Abbasi |
| collection | DOAJ |
| description | In the present study, a functional template made up of in situ synthesised gold nanoparticles (AuNPs) is prepared on polydimethylsiloxane (PDMS) for patterning of target protein onto the desired solid substrates. Unlike previous studies in which bioreceptor probes are randomly attached to the PDMS stamp through electrostatic interactions, herein, we propose an AuNPs–PDMS stamp, which provides a surface for the attachment of thiol-modified biorecognition probes to link to the stamp surface through a dative bond with a single anchoring point based on thiol chemistry. By using this platform, we have developed the ability for microcontact printing (µCP) to selectively capture and transfer target protein onto solid surfaces for detection purposes. After µCP, we also investigated whether liquid crystals (LCs) could be used as a label-free approach for identifying transfer protein. Our reported approach provides promise for biosensing of various analytes. |
| first_indexed | 2024-03-09T17:16:08Z |
| format | Article |
| id | doaj.art-56f003263fac4d28a371763f05dfbb2d |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T17:16:08Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-56f003263fac4d28a371763f05dfbb2d2023-11-24T13:35:54ZengMDPI AGBiosensors2079-63742022-11-011212106710.3390/bios12121067Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact PrintingAmna Didar Abbasi0Zakir Hussain1Kun-Lin Yang2Department of Materials Engineering, School of Chemical and Materials Engineering (SCME), National University of Sciences and Technology (NUST), Islamabad 44000, PakistanDepartment of Materials Engineering, School of Chemical and Materials Engineering (SCME), National University of Sciences and Technology (NUST), Islamabad 44000, PakistanDepartment of Chemical and Biomolecular Engineering, National University of Singapore, Engineering Drive 4, Singapore 117576, SingaporeIn the present study, a functional template made up of in situ synthesised gold nanoparticles (AuNPs) is prepared on polydimethylsiloxane (PDMS) for patterning of target protein onto the desired solid substrates. Unlike previous studies in which bioreceptor probes are randomly attached to the PDMS stamp through electrostatic interactions, herein, we propose an AuNPs–PDMS stamp, which provides a surface for the attachment of thiol-modified biorecognition probes to link to the stamp surface through a dative bond with a single anchoring point based on thiol chemistry. By using this platform, we have developed the ability for microcontact printing (µCP) to selectively capture and transfer target protein onto solid surfaces for detection purposes. After µCP, we also investigated whether liquid crystals (LCs) could be used as a label-free approach for identifying transfer protein. Our reported approach provides promise for biosensing of various analytes.https://www.mdpi.com/2079-6374/12/12/1067liquid crystalsDMOAPAuNPs–PDMS compositemicro-contact printing |
| spellingShingle | Amna Didar Abbasi Zakir Hussain Kun-Lin Yang Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing Biosensors liquid crystals DMOAP AuNPs–PDMS composite micro-contact printing |
| title | Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing |
| title_full | Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing |
| title_fullStr | Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing |
| title_full_unstemmed | Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing |
| title_short | Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing |
| title_sort | aptamer based gold nanoparticles pdms composite stamps as a platform for micro contact printing |
| topic | liquid crystals DMOAP AuNPs–PDMS composite micro-contact printing |
| url | https://www.mdpi.com/2079-6374/12/12/1067 |
| work_keys_str_mv | AT amnadidarabbasi aptamerbasedgoldnanoparticlespdmscompositestampsasaplatformformicrocontactprinting AT zakirhussain aptamerbasedgoldnanoparticlespdmscompositestampsasaplatformformicrocontactprinting AT kunlinyang aptamerbasedgoldnanoparticlespdmscompositestampsasaplatformformicrocontactprinting |