Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus)
Pineapple (Ananas comosus) is commonly infected by Fusarium oxysporum, causal agent of the fusarium wilt disease. Conventionally, growers use synthetic fungicides to control the disease, which lead to environmental pollution, hazardous effects on non-target organisms and risks on human health. The a...
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PeerJ Inc.
2024-03-01
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| author | Lourdes Adriano-Anaya Luis Fernando Pardo-Girón Miguel Salvador-Adriano Miguel Salvador-Figueroa Isidro Ovando-Medina Benjamin Moreno-Castillo |
| author_facet | Lourdes Adriano-Anaya Luis Fernando Pardo-Girón Miguel Salvador-Adriano Miguel Salvador-Figueroa Isidro Ovando-Medina Benjamin Moreno-Castillo |
| author_sort | Lourdes Adriano-Anaya |
| collection | DOAJ |
| description | Pineapple (Ananas comosus) is commonly infected by Fusarium oxysporum, causal agent of the fusarium wilt disease. Conventionally, growers use synthetic fungicides to control the disease, which lead to environmental pollution, hazardous effects on non-target organisms and risks on human health. The aim of this work was to assess the effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B to control fusarium wilt on pineapple plants. Four treatments derived from a complete factorial design were tested under field conditions. Treatments composed of B. subtilis ANT01 and the combination B. subtilis ANT01–Rhizobium sp. 11B decreased disease severity by 94.4% and 86.1%, respectively. On the other hand, the treatment prepared with Rhizobium sp. 11B alone showed a reduction of 75.0%. Size of leaves and nutritional condition (SPAD units) of the biocontrol agents-treated plants showed no statistical differences. Moreover, B. subtilis ANT01 decreased by 46% the initial soil population of F. oxysporum, while Rhizobium sp. 11B, B. subtilis ANT01 plus Rhizobium sp. 11B and control, showed a population reduction of 12.5%, 24.2% and 23.0%, respectively. These results make evident the potential of B. subtilis ANT01 as biocontrol agent of the pathogen under field conditions. |
| first_indexed | 2024-04-25T02:00:29Z |
| format | Article |
| id | doaj.art-56f245a962f04dcf80368995e8d7f787 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-25T02:00:29Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-56f245a962f04dcf80368995e8d7f7872024-03-07T15:05:10ZengPeerJ Inc.PeerJ2167-83592024-03-0112e1687110.7717/peerj.16871Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus)Lourdes Adriano-Anaya0Luis Fernando Pardo-Girón1Miguel Salvador-Adriano2Miguel Salvador-Figueroa3Isidro Ovando-Medina4Benjamin Moreno-Castillo5Instituto de Biociencias, Campus IV, Universidad Autónoma de Chiapas, Tapachula, Chiapas, MexicoInstituto de Biociencias, Campus IV, Universidad Autónoma de Chiapas, Tapachula, Chiapas, MexicoInstituto de Biociencias, Campus IV, Universidad Autónoma de Chiapas, Tapachula, Chiapas, MexicoInstituto de Biociencias, Campus IV, Universidad Autónoma de Chiapas, Tapachula, Chiapas, MexicoInstituto de Biociencias, Campus IV, Universidad Autónoma de Chiapas, Tapachula, Chiapas, MexicoInstituto de Biociencias, Campus IV, Universidad Autónoma de Chiapas, Tapachula, Chiapas, MexicoPineapple (Ananas comosus) is commonly infected by Fusarium oxysporum, causal agent of the fusarium wilt disease. Conventionally, growers use synthetic fungicides to control the disease, which lead to environmental pollution, hazardous effects on non-target organisms and risks on human health. The aim of this work was to assess the effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B to control fusarium wilt on pineapple plants. Four treatments derived from a complete factorial design were tested under field conditions. Treatments composed of B. subtilis ANT01 and the combination B. subtilis ANT01–Rhizobium sp. 11B decreased disease severity by 94.4% and 86.1%, respectively. On the other hand, the treatment prepared with Rhizobium sp. 11B alone showed a reduction of 75.0%. Size of leaves and nutritional condition (SPAD units) of the biocontrol agents-treated plants showed no statistical differences. Moreover, B. subtilis ANT01 decreased by 46% the initial soil population of F. oxysporum, while Rhizobium sp. 11B, B. subtilis ANT01 plus Rhizobium sp. 11B and control, showed a population reduction of 12.5%, 24.2% and 23.0%, respectively. These results make evident the potential of B. subtilis ANT01 as biocontrol agent of the pathogen under field conditions.https://peerj.com/articles/16871.pdfInoculumSporesPlant pathogenBiocontrol |
| spellingShingle | Lourdes Adriano-Anaya Luis Fernando Pardo-Girón Miguel Salvador-Adriano Miguel Salvador-Figueroa Isidro Ovando-Medina Benjamin Moreno-Castillo Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus) PeerJ Inoculum Spores Plant pathogen Biocontrol |
| title | Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus) |
| title_full | Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus) |
| title_fullStr | Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus) |
| title_full_unstemmed | Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus) |
| title_short | Effectiveness of Bacillus subtilis ANT01 and Rhizobium sp. 11B on the control of fusarium wilt in pineapple (Ananas comosus) |
| title_sort | effectiveness of bacillus subtilis ant01 and rhizobium sp 11b on the control of fusarium wilt in pineapple ananas comosus |
| topic | Inoculum Spores Plant pathogen Biocontrol |
| url | https://peerj.com/articles/16871.pdf |
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