A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case
Natural peptides (NPs) are ubiquitous throughout the organism with diversified structures and wide dynamic concentration range, which leads to serious challenge for their comprehensive analysis. Herein, a pseudotargeted peptidomics strategy based on dynamic multiple reaction monitoring (DMRM) with h...
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Format: | Article |
Language: | English |
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Elsevier
2023-08-01
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Series: | Arabian Journal of Chemistry |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223004422 |
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author | Dongdong Huang Xiaoxiao Luo Qirui Bi Yelin Ding Yun Li Cuicui Wang Min Gao Yong Huang Changliang Yao Jianqing Zhang Wenlong Wei Yurong Wang De-an Guo |
author_facet | Dongdong Huang Xiaoxiao Luo Qirui Bi Yelin Ding Yun Li Cuicui Wang Min Gao Yong Huang Changliang Yao Jianqing Zhang Wenlong Wei Yurong Wang De-an Guo |
author_sort | Dongdong Huang |
collection | DOAJ |
description | Natural peptides (NPs) are ubiquitous throughout the organism with diversified structures and wide dynamic concentration range, which leads to serious challenge for their comprehensive analysis. Herein, a pseudotargeted peptidomics strategy based on dynamic multiple reaction monitoring (DMRM) with high-throughput data acquisition and accurate quantification was established. Specifically, an in-house software Pep-MRMer was developed for extraction of ions information of peptides, followed by de-redundancy and fusion to construct a transition list. Additionally, retention time (RT) calibration method was optimized by using high content components for effective transference of ion pairs between different detection conditions (e.g., instruments, chromatographic gradients). This strategy was applied for profiling NPs and screening signature peptides for species authentication of Pheretima, a multi-source animal-derived drug widely used in Asian countries. In all, a total of 3307 peptides had excellent quantitative detection after transference. Subsequently, nine signature peptides with extraordinary specificity were screened, identified, and confirmed by comparing with the synthetic standards. Ultimately, a 10 min specific chromatogram based on signature peptides was developed, which realized rapid and accurate identification of Pheretima. Taken together, this research provides a robust strategy for species discrimination of animal-derived drugs without adequate quality control measures. |
first_indexed | 2024-03-13T05:29:20Z |
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institution | Directory Open Access Journal |
issn | 1878-5352 |
language | English |
last_indexed | 2024-03-13T05:29:20Z |
publishDate | 2023-08-01 |
publisher | Elsevier |
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series | Arabian Journal of Chemistry |
spelling | doaj.art-56f2d31413d74d5e865f6405b70d0dc32023-06-15T04:55:09ZengElsevierArabian Journal of Chemistry1878-53522023-08-01168104980A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a caseDongdong Huang0Xiaoxiao Luo1Qirui Bi2Yelin Ding3Yun Li4Cuicui Wang5Min Gao6Yong Huang7Changliang Yao8Jianqing Zhang9Wenlong Wei10Yurong Wang11De-an Guo12Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; China Pharmaceutical University, Nanjing 211198, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaChina Pharmaceutical University, Nanjing 211198, China; Corresponding authors at: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai 201203, China. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China; Corresponding authors at: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai 201203, China. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.Natural peptides (NPs) are ubiquitous throughout the organism with diversified structures and wide dynamic concentration range, which leads to serious challenge for their comprehensive analysis. Herein, a pseudotargeted peptidomics strategy based on dynamic multiple reaction monitoring (DMRM) with high-throughput data acquisition and accurate quantification was established. Specifically, an in-house software Pep-MRMer was developed for extraction of ions information of peptides, followed by de-redundancy and fusion to construct a transition list. Additionally, retention time (RT) calibration method was optimized by using high content components for effective transference of ion pairs between different detection conditions (e.g., instruments, chromatographic gradients). This strategy was applied for profiling NPs and screening signature peptides for species authentication of Pheretima, a multi-source animal-derived drug widely used in Asian countries. In all, a total of 3307 peptides had excellent quantitative detection after transference. Subsequently, nine signature peptides with extraordinary specificity were screened, identified, and confirmed by comparing with the synthetic standards. Ultimately, a 10 min specific chromatogram based on signature peptides was developed, which realized rapid and accurate identification of Pheretima. Taken together, this research provides a robust strategy for species discrimination of animal-derived drugs without adequate quality control measures.http://www.sciencedirect.com/science/article/pii/S1878535223004422Natural peptidesPseudotargeted peptidomicsSignature peptidesAnimal-derived drugsPheretima |
spellingShingle | Dongdong Huang Xiaoxiao Luo Qirui Bi Yelin Ding Yun Li Cuicui Wang Min Gao Yong Huang Changliang Yao Jianqing Zhang Wenlong Wei Yurong Wang De-an Guo A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case Arabian Journal of Chemistry Natural peptides Pseudotargeted peptidomics Signature peptides Animal-derived drugs Pheretima |
title | A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case |
title_full | A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case |
title_fullStr | A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case |
title_full_unstemmed | A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case |
title_short | A pseudotargeted peptidomics strategy for screening natural signature peptides in animal-derived drugs: Taking Pheretima as a case |
title_sort | pseudotargeted peptidomics strategy for screening natural signature peptides in animal derived drugs taking pheretima as a case |
topic | Natural peptides Pseudotargeted peptidomics Signature peptides Animal-derived drugs Pheretima |
url | http://www.sciencedirect.com/science/article/pii/S1878535223004422 |
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