Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice
The inhalation of zinc chloride (ZnCl2) smoke is one of common resources of lung injury, potentially resulting in severe pulmonary complications and even mortality. The influence of ZnCl2 smoke on lysine succinylation (Ksucc) in the lungs remains uncertain. In this study, we used a ZnCl2 smoke inhal...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024034819 |
| _version_ | 1797224262793166848 |
|---|---|
| author | Rui Zhou Zhiwei Tu Daishi Chen Wanmei Wang Shuzi Liu Linjun She Zhan Li Jihong Liu Yabin Li Yu Cui Pan Pan Fei Xie |
| author_facet | Rui Zhou Zhiwei Tu Daishi Chen Wanmei Wang Shuzi Liu Linjun She Zhan Li Jihong Liu Yabin Li Yu Cui Pan Pan Fei Xie |
| author_sort | Rui Zhou |
| collection | DOAJ |
| description | The inhalation of zinc chloride (ZnCl2) smoke is one of common resources of lung injury, potentially resulting in severe pulmonary complications and even mortality. The influence of ZnCl2 smoke on lysine succinylation (Ksucc) in the lungs remains uncertain. In this study, we used a ZnCl2 smoke inhalation mouse model to perform global proteomic and lysine succinylome analyses. A total of 6781 Ksucc sites were identified in the lungs, with injured lungs demonstrating a reduction to approximately 2000 Ksucc sites, and 91 proteins exhibiting at least five differences in the number of Ksucc sites. Quantitative analysis revealed variations in expression of 384 proteins and 749 Ksucc sites. The analysis of protein-protein interactions was conducted for proteins displaying differential expression and differentially expressed lysine succinylation. Notably, proteins with altered Ksucc exhibited increased connectivity compared with that in differentially expressed proteins. Beyond metabolic pathways, these highly connected proteins were also involved in lung injury-associated pathological reactions, including processes such as focal adhesion, adherens junction, and complement and coagulation cascades. Collectively, our findings contribute to the understanding of the molecular mechanisms underlaying ZnCl2 smoke-induced lung injury with a specific emphasis on lysine succinylation. These findings could pave the way for targeted interventions and therapeutic strategies to mitigate severe pulmonary complications and mortality associated with such injuries in humans. |
| first_indexed | 2024-04-24T13:50:20Z |
| format | Article |
| id | doaj.art-56f2d684207c4d2f8f613c11ca59f2ae |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:20Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-56f2d684207c4d2f8f613c11ca59f2ae2024-04-04T05:04:52ZengElsevierHeliyon2405-84402024-03-01106e27450Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in miceRui Zhou0Zhiwei Tu1Daishi Chen2Wanmei Wang3Shuzi Liu4Linjun She5Zhan Li6Jihong Liu7Yabin Li8Yu Cui9Pan Pan10Fei Xie11The First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, ChinaNational Center for Protein Sciences (Beijing), Institute of Lifeomics, 102206, Beijing, ChinaDepartment of Otorhinolaryngology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, 515100, Shenzhen, Guangdong, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 100850, Beijing, ChinaCollege of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, ChinaThe First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, ChinaThe First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, ChinaThe First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, ChinaCollege of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, ChinaNational Center for Protein Sciences (Beijing), Institute of Lifeomics, 102206, Beijing, China; State Key Laboratory of Proteomics, 102206, Beijing, China; Corresponding author. National Center for Protein Sciences (Beijing), Institute of Lifeomics, 102206, Beijing, China.College of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, China; Corresponding author. College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, 100048, Beijing, China.College of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, China; Corresponding author. College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, 100048, Beijing, China.The inhalation of zinc chloride (ZnCl2) smoke is one of common resources of lung injury, potentially resulting in severe pulmonary complications and even mortality. The influence of ZnCl2 smoke on lysine succinylation (Ksucc) in the lungs remains uncertain. In this study, we used a ZnCl2 smoke inhalation mouse model to perform global proteomic and lysine succinylome analyses. A total of 6781 Ksucc sites were identified in the lungs, with injured lungs demonstrating a reduction to approximately 2000 Ksucc sites, and 91 proteins exhibiting at least five differences in the number of Ksucc sites. Quantitative analysis revealed variations in expression of 384 proteins and 749 Ksucc sites. The analysis of protein-protein interactions was conducted for proteins displaying differential expression and differentially expressed lysine succinylation. Notably, proteins with altered Ksucc exhibited increased connectivity compared with that in differentially expressed proteins. Beyond metabolic pathways, these highly connected proteins were also involved in lung injury-associated pathological reactions, including processes such as focal adhesion, adherens junction, and complement and coagulation cascades. Collectively, our findings contribute to the understanding of the molecular mechanisms underlaying ZnCl2 smoke-induced lung injury with a specific emphasis on lysine succinylation. These findings could pave the way for targeted interventions and therapeutic strategies to mitigate severe pulmonary complications and mortality associated with such injuries in humans.http://www.sciencedirect.com/science/article/pii/S2405844024034819Lysine succinylationKsuccSuccinylomeLung injuryZinc chloride smoke |
| spellingShingle | Rui Zhou Zhiwei Tu Daishi Chen Wanmei Wang Shuzi Liu Linjun She Zhan Li Jihong Liu Yabin Li Yu Cui Pan Pan Fei Xie Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice Heliyon Lysine succinylation Ksucc Succinylome Lung injury Zinc chloride smoke |
| title | Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice |
| title_full | Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice |
| title_fullStr | Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice |
| title_full_unstemmed | Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice |
| title_short | Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice |
| title_sort | quantitative proteome and lysine succinylome characterization of zinc chloride smoke induced lung injury in mice |
| topic | Lysine succinylation Ksucc Succinylome Lung injury Zinc chloride smoke |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024034819 |
| work_keys_str_mv | AT ruizhou quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT zhiweitu quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT daishichen quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT wanmeiwang quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT shuziliu quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT linjunshe quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT zhanli quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT jihongliu quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT yabinli quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT yucui quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT panpan quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice AT feixie quantitativeproteomeandlysinesuccinylomecharacterizationofzincchloridesmokeinducedlunginjuryinmice |