Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons
Introduction: Patient-derived induced pluripotent stem cells (iPSCs) have been widely used as disease models to test new therapeutic strategies. Moreover, the regenerative potential of stem cells can be improved with the use of biologically active compounds. Our study was designed to explore the eff...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2024-01-01
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| Series: | BioImpacts |
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| Online Access: | https://bi.tbzmed.ac.ir/PDF/bi-14-27652.pdf |
| _version_ | 1797323315768983552 |
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| author | Duong Thi Thuy Le Cuong Manh Vu Thuy Thi Bich Ly Nam Trung Nguyen Phuong Thi Mai Nguyen Ha Hoang Chu |
| author_facet | Duong Thi Thuy Le Cuong Manh Vu Thuy Thi Bich Ly Nam Trung Nguyen Phuong Thi Mai Nguyen Ha Hoang Chu |
| author_sort | Duong Thi Thuy Le |
| collection | DOAJ |
| description | Introduction: Patient-derived induced pluripotent stem cells (iPSCs) have been widely used as disease models to test new therapeutic strategies. Moreover, the regenerative potential of stem cells can be improved with the use of biologically active compounds. Our study was designed to explore the effect of honokiol, a small polyphenol molecule extracted from Magnolia officinalis, on the survival and culture time of iPSC-derived neurons from a sporadic Alzheimer’s disease (AD) patient. This study aimed to generate iPSCs from peripheral blood mononuclear cells (PBMCs) of an AD patient using episomal plasmids with a nucleofector system and differentiate them into neurons. These iPSC-derived neurons were used to investigate the effect of honokiol extracted from M. officinalis on their survival and long-term cultures. Methods: IPSCs were generated from PBMCs of an AD patient by introducing Oct-3/4, Sox2, Klf4, L-Myc, and Lin28 using Nucleofector™ Technology. Differentiation of neurons derived from iPSCs was carried out using inducers and recognized by biomarkers. The viability of iPSC-derived neurons with the addition of honokiol extracted from the bark of M. officinalis was determined by the MTT analytical kit. Results: IPSCs were generated by reprogramming AD patient-derived PBMCs and subsequently converted into neurons. The survival and growth of iPSC-derived neurons were significantly enhanced by adding honokiol in the experiment conditions. Conclusion: AD iPSC-derived neurons had a high viability rate when cultured in the presence of honokiol. These results have shown that AD iPSC-derived neurons can be an excellent model for screening neurotrophic agents and improving the conditions for long-term cultures of human iPSC-derived neurons. Honokiol proves to be a potential candidate for cellular therapeutics against neurodegenerative disorders. |
| first_indexed | 2024-03-08T05:26:59Z |
| format | Article |
| id | doaj.art-56f3e5a2ebf541a78696a220c10ddfd6 |
| institution | Directory Open Access Journal |
| issn | 2228-5652 2228-5660 |
| language | English |
| last_indexed | 2024-03-08T05:26:59Z |
| publishDate | 2024-01-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj.art-56f3e5a2ebf541a78696a220c10ddfd62024-02-06T10:14:28ZengTabriz University of Medical SciencesBioImpacts2228-56522228-56602024-01-01141276522765210.34172/bi.2023.27652bi-27652Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neuronsDuong Thi Thuy Le0Cuong Manh Vu1Thuy Thi Bich Ly2Nam Trung Nguyen3Phuong Thi Mai Nguyen4Ha Hoang Chu5Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, VietnamIntroduction: Patient-derived induced pluripotent stem cells (iPSCs) have been widely used as disease models to test new therapeutic strategies. Moreover, the regenerative potential of stem cells can be improved with the use of biologically active compounds. Our study was designed to explore the effect of honokiol, a small polyphenol molecule extracted from Magnolia officinalis, on the survival and culture time of iPSC-derived neurons from a sporadic Alzheimer’s disease (AD) patient. This study aimed to generate iPSCs from peripheral blood mononuclear cells (PBMCs) of an AD patient using episomal plasmids with a nucleofector system and differentiate them into neurons. These iPSC-derived neurons were used to investigate the effect of honokiol extracted from M. officinalis on their survival and long-term cultures. Methods: IPSCs were generated from PBMCs of an AD patient by introducing Oct-3/4, Sox2, Klf4, L-Myc, and Lin28 using Nucleofector™ Technology. Differentiation of neurons derived from iPSCs was carried out using inducers and recognized by biomarkers. The viability of iPSC-derived neurons with the addition of honokiol extracted from the bark of M. officinalis was determined by the MTT analytical kit. Results: IPSCs were generated by reprogramming AD patient-derived PBMCs and subsequently converted into neurons. The survival and growth of iPSC-derived neurons were significantly enhanced by adding honokiol in the experiment conditions. Conclusion: AD iPSC-derived neurons had a high viability rate when cultured in the presence of honokiol. These results have shown that AD iPSC-derived neurons can be an excellent model for screening neurotrophic agents and improving the conditions for long-term cultures of human iPSC-derived neurons. Honokiol proves to be a potential candidate for cellular therapeutics against neurodegenerative disorders.https://bi.tbzmed.ac.ir/PDF/bi-14-27652.pdfepisomal plasmidinduced pluripotent stem cellspbmcsneuronal differentiationneurotrophic agenthonokiol |
| spellingShingle | Duong Thi Thuy Le Cuong Manh Vu Thuy Thi Bich Ly Nam Trung Nguyen Phuong Thi Mai Nguyen Ha Hoang Chu Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons BioImpacts episomal plasmid induced pluripotent stem cells pbmcs neuronal differentiation neurotrophic agent honokiol |
| title | Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons |
| title_full | Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons |
| title_fullStr | Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons |
| title_full_unstemmed | Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons |
| title_short | Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons |
| title_sort | effect of honokiol on culture time and survival of alzheimer s disease ipsc derived neurons |
| topic | episomal plasmid induced pluripotent stem cells pbmcs neuronal differentiation neurotrophic agent honokiol |
| url | https://bi.tbzmed.ac.ir/PDF/bi-14-27652.pdf |
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