Differential bacterial surface display of peptides by the transmembrane domain of OmpA.
Peptide libraries or antigenic determinants can be displayed on the surface of bacteria through insertion in a suitable outer membrane scaffold protein. Here, we inserted the well-known antibody epitopes 3xFLAG and 2xmyc in exterior loops of the transmembrane (TM) domain of OmpA. Although these high...
Main Authors: | Gertjan S Verhoeven, Svetlana Alexeeva, Marileen Dogterom, Tanneke den Blaauwen |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2009-08-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2726941?pdf=render |
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