Differential bacterial surface display of peptides by the transmembrane domain of OmpA.

Peptide libraries or antigenic determinants can be displayed on the surface of bacteria through insertion in a suitable outer membrane scaffold protein. Here, we inserted the well-known antibody epitopes 3xFLAG and 2xmyc in exterior loops of the transmembrane (TM) domain of OmpA. Although these high...

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Bibliographic Details
Main Authors: Gertjan S Verhoeven, Svetlana Alexeeva, Marileen Dogterom, Tanneke den Blaauwen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-08-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2726941?pdf=render

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