Proteinase activity in latex of three plants of the family Euphorbiaceae
In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi(Klotzsch & Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, wer...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2014-09-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300559&lng=en&tlng=en |
| _version_ | 1811333879210967040 |
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| author | Andréa Michel Sobottka Fabiana Tonial Sonja Sytwala Matthias Melzig |
| author_facet | Andréa Michel Sobottka Fabiana Tonial Sonja Sytwala Matthias Melzig |
| author_sort | Andréa Michel Sobottka |
| collection | DOAJ |
| description | In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi(Klotzsch & Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, were examined concerning proteolytic activity. All studied species have proteins with significant proteolytic activity and E. papillosa has the greatest specific activity. Aiming to verify the type of protease present, an assay with different inhibitors was performed. In the three tested plants, the proteolytic activity was significantly inhibited by a serine protease inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride (AEBSF). Using techniques of electrophoresis with polyacrylamide gels (SDS-PAGE), the subunits of proteins were separated according to their molecular masses, and the protein activity was visually detected by zymography. |
| first_indexed | 2024-04-13T16:59:27Z |
| format | Article |
| id | doaj.art-56fce07cfad54ccf90b1111cf7bc0a0b |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-13T16:59:27Z |
| publishDate | 2014-09-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-56fce07cfad54ccf90b1111cf7bc0a0b2022-12-22T02:38:42ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902014-09-0150355956510.1590/S1984-82502014000300015S1984-82502014000300559Proteinase activity in latex of three plants of the family EuphorbiaceaeAndréa Michel SobottkaFabiana TonialSonja SytwalaMatthias MelzigIn the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi(Klotzsch & Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, were examined concerning proteolytic activity. All studied species have proteins with significant proteolytic activity and E. papillosa has the greatest specific activity. Aiming to verify the type of protease present, an assay with different inhibitors was performed. In the three tested plants, the proteolytic activity was significantly inhibited by a serine protease inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride (AEBSF). Using techniques of electrophoresis with polyacrylamide gels (SDS-PAGE), the subunits of proteins were separated according to their molecular masses, and the protein activity was visually detected by zymography.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300559&lng=en&tlng=enEuphorbiaceae/espécies/fitoquímicaEuphorbia papillosa/fitoquímicaEuphorbia selloi/fitoquímicaSapium glandulosum/fitoquímicaEuphorbia papillosa/atividade proteolíticaEuphorbia selloi/atividade proteolíticaSapium glandulosum/atividade proteolíticaEndopeptidasesEletroforese em gel |
| spellingShingle | Andréa Michel Sobottka Fabiana Tonial Sonja Sytwala Matthias Melzig Proteinase activity in latex of three plants of the family Euphorbiaceae Brazilian Journal of Pharmaceutical Sciences Euphorbiaceae/espécies/fitoquímica Euphorbia papillosa/fitoquímica Euphorbia selloi/fitoquímica Sapium glandulosum/fitoquímica Euphorbia papillosa/atividade proteolítica Euphorbia selloi/atividade proteolítica Sapium glandulosum/atividade proteolítica Endopeptidases Eletroforese em gel |
| title | Proteinase activity in latex of three plants of the family Euphorbiaceae |
| title_full | Proteinase activity in latex of three plants of the family Euphorbiaceae |
| title_fullStr | Proteinase activity in latex of three plants of the family Euphorbiaceae |
| title_full_unstemmed | Proteinase activity in latex of three plants of the family Euphorbiaceae |
| title_short | Proteinase activity in latex of three plants of the family Euphorbiaceae |
| title_sort | proteinase activity in latex of three plants of the family euphorbiaceae |
| topic | Euphorbiaceae/espécies/fitoquímica Euphorbia papillosa/fitoquímica Euphorbia selloi/fitoquímica Sapium glandulosum/fitoquímica Euphorbia papillosa/atividade proteolítica Euphorbia selloi/atividade proteolítica Sapium glandulosum/atividade proteolítica Endopeptidases Eletroforese em gel |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300559&lng=en&tlng=en |
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