Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy
Abstract Aronia melanocarpa is rich in anthocyanin, among which cyanidin‐3‐galactoside (C3G) is the most abundant. Here, we established an experimental model of mice and alveolar macrophage cell line MH‐S exposed to PM10, and C3G was administered to explore the underlying mechanism of C3G exerting p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | eFood |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/efd2.119 |
| _version_ | 1797397346542157824 |
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| author | Xia Liu Xiaowei Zhang Yan Gao Jinlong Tian Jin Zhao |
| author_facet | Xia Liu Xiaowei Zhang Yan Gao Jinlong Tian Jin Zhao |
| author_sort | Xia Liu |
| collection | DOAJ |
| description | Abstract Aronia melanocarpa is rich in anthocyanin, among which cyanidin‐3‐galactoside (C3G) is the most abundant. Here, we established an experimental model of mice and alveolar macrophage cell line MH‐S exposed to PM10, and C3G was administered to explore the underlying mechanism of C3G exerting protective effects on PM10‐induced lung inflammation. The results showed C3G alleviated PM10‐induced lung inflammation by reducing matrix metalloproteinases production at both gene and protein levels as well as pro‐inflammatory cytokine levels. Autophagy was activated in PM10‐injured alveolar macrophages (AMs), and C3G promoted autophagy indicated by LC3, BECN1, Atg5, Bcl‐2, and P62 expression, and C3G further resisted PM10‐induced AMs apoptosis. Besides, C3G could promote Pink1/Parkin‐mediated mitochondrial autophagy of AMs to alleviate the overexpression of mitochondrial reactive oxygen species (ROS) so that maintain mitochondrial membrane potential and ATP productions to restrict the distribution of Cyt‐c and further reduce the increasing of caspase 3/7 activity. Therefore, C3G enhanced the clearance of PM10‐damaged mitochondria by promoting AMs mitophagy, thereby reducing the activation of mitochondria‐dependent apoptosis pathway and the production of excess ROS, which reduced AMs apoptosis and secretion of pro‐inflammatory factors to ameliorate PM10‐induced pulmonary inflammation, suggesting that C3G may be as a functional food ingredient with the potential to improve lung health. |
| first_indexed | 2024-03-09T01:08:34Z |
| format | Article |
| id | doaj.art-56fd20693e904b5da3d9ecb99b77a309 |
| institution | Directory Open Access Journal |
| issn | 2666-3066 |
| language | English |
| last_indexed | 2024-03-09T01:08:34Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | eFood |
| spelling | doaj.art-56fd20693e904b5da3d9ecb99b77a3092023-12-11T08:56:28ZengWileyeFood2666-30662023-12-0146n/an/a10.1002/efd2.119Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagyXia Liu0Xiaowei Zhang1Yan Gao2Jinlong Tian3Jin Zhao4College of Food Science Shenyang Agricultural University Shenyang Liaoning ChinaCollege of Food Science Shenyang Agricultural University Shenyang Liaoning ChinaCollege of Food Science Shenyang Agricultural University Shenyang Liaoning ChinaCollege of Food Science Shenyang Agricultural University Shenyang Liaoning ChinaCollege of Food Science Shenyang Agricultural University Shenyang Liaoning ChinaAbstract Aronia melanocarpa is rich in anthocyanin, among which cyanidin‐3‐galactoside (C3G) is the most abundant. Here, we established an experimental model of mice and alveolar macrophage cell line MH‐S exposed to PM10, and C3G was administered to explore the underlying mechanism of C3G exerting protective effects on PM10‐induced lung inflammation. The results showed C3G alleviated PM10‐induced lung inflammation by reducing matrix metalloproteinases production at both gene and protein levels as well as pro‐inflammatory cytokine levels. Autophagy was activated in PM10‐injured alveolar macrophages (AMs), and C3G promoted autophagy indicated by LC3, BECN1, Atg5, Bcl‐2, and P62 expression, and C3G further resisted PM10‐induced AMs apoptosis. Besides, C3G could promote Pink1/Parkin‐mediated mitochondrial autophagy of AMs to alleviate the overexpression of mitochondrial reactive oxygen species (ROS) so that maintain mitochondrial membrane potential and ATP productions to restrict the distribution of Cyt‐c and further reduce the increasing of caspase 3/7 activity. Therefore, C3G enhanced the clearance of PM10‐damaged mitochondria by promoting AMs mitophagy, thereby reducing the activation of mitochondria‐dependent apoptosis pathway and the production of excess ROS, which reduced AMs apoptosis and secretion of pro‐inflammatory factors to ameliorate PM10‐induced pulmonary inflammation, suggesting that C3G may be as a functional food ingredient with the potential to improve lung health.https://doi.org/10.1002/efd2.119alveolar macrophagescyanidin‐3‐galactosidemitophagyPINK1/Parkin signaling pathwayPM10pulmonary inflammation |
| spellingShingle | Xia Liu Xiaowei Zhang Yan Gao Jinlong Tian Jin Zhao Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy eFood alveolar macrophages cyanidin‐3‐galactoside mitophagy PINK1/Parkin signaling pathway PM10 pulmonary inflammation |
| title | Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy |
| title_full | Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy |
| title_fullStr | Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy |
| title_full_unstemmed | Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy |
| title_short | Cyanidin‐3‐galactoside from Aronia melanocarpa ameliorates PM10‐induced pulmonary inflammation by promoting PINK1/Parkin signaling pathway‐mediated alveolar macrophage mitophagy |
| title_sort | cyanidin 3 galactoside from aronia melanocarpa ameliorates pm10 induced pulmonary inflammation by promoting pink1 parkin signaling pathway mediated alveolar macrophage mitophagy |
| topic | alveolar macrophages cyanidin‐3‐galactoside mitophagy PINK1/Parkin signaling pathway PM10 pulmonary inflammation |
| url | https://doi.org/10.1002/efd2.119 |
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