Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents
In seeds, the endosperm is a crucial organ that plays vital roles in supporting embryo development and determining seed weight and quality. Starch is the predominant storage carbohydrate of the endosperm and accounts for ∼70% of the mature maize kernel weight. Nonetheless, because starch biosynthesi...
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PeerJ Inc.
2019-08-01
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| author | Jianzhou Qu Shutu Xu Xiaokang Tian Ting Li Licheng Wang Yuyue Zhong Jiquan Xue Dongwei Guo |
| author_facet | Jianzhou Qu Shutu Xu Xiaokang Tian Ting Li Licheng Wang Yuyue Zhong Jiquan Xue Dongwei Guo |
| author_sort | Jianzhou Qu |
| collection | DOAJ |
| description | In seeds, the endosperm is a crucial organ that plays vital roles in supporting embryo development and determining seed weight and quality. Starch is the predominant storage carbohydrate of the endosperm and accounts for ∼70% of the mature maize kernel weight. Nonetheless, because starch biosynthesis is a complex process that is orchestrated by multiple enzymes, the gene regulatory networks of starch biosynthesis, particularly amylose and amylopectin biosynthesis, have not been fully elucidated. Here, through high-throughput RNA sequencing, we developed a temporal transcriptome atlas of the endosperms of high-amylose maize and common maize at 5-, 10-, 15- and 20-day after pollination and found that 21,986 genes are involved in the programming of the high-amylose and common maize endosperm. A coexpression analysis identified multiple sequentially expressed gene sets that are closely correlated with cellular and metabolic programmes and provided valuable insight into the dynamic reprogramming of the transcriptome in common and high-amylose maize. In addition, a number of genes and transcription factors were found to be strongly linked to starch synthesis, which might help elucidate the key mechanisms and regulatory networks underlying amylose and amylopectin biosynthesis. This study will aid the understanding of the spatiotemporal patterns and genetic regulation of endosperm development in different types of maize and provide valuable genetic information for the breeding of starch varieties with different contents. |
| first_indexed | 2024-03-09T07:23:58Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:23:58Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-56fd433ed0954b7a84ed1b83b534383e2023-12-03T07:08:56ZengPeerJ Inc.PeerJ2167-83592019-08-017e752810.7717/peerj.7528Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contentsJianzhou Qu0Shutu Xu1Xiaokang Tian2Ting Li3Licheng Wang4Yuyue Zhong5Jiquan Xue6Dongwei Guo7The Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaThe Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, ChinaIn seeds, the endosperm is a crucial organ that plays vital roles in supporting embryo development and determining seed weight and quality. Starch is the predominant storage carbohydrate of the endosperm and accounts for ∼70% of the mature maize kernel weight. Nonetheless, because starch biosynthesis is a complex process that is orchestrated by multiple enzymes, the gene regulatory networks of starch biosynthesis, particularly amylose and amylopectin biosynthesis, have not been fully elucidated. Here, through high-throughput RNA sequencing, we developed a temporal transcriptome atlas of the endosperms of high-amylose maize and common maize at 5-, 10-, 15- and 20-day after pollination and found that 21,986 genes are involved in the programming of the high-amylose and common maize endosperm. A coexpression analysis identified multiple sequentially expressed gene sets that are closely correlated with cellular and metabolic programmes and provided valuable insight into the dynamic reprogramming of the transcriptome in common and high-amylose maize. In addition, a number of genes and transcription factors were found to be strongly linked to starch synthesis, which might help elucidate the key mechanisms and regulatory networks underlying amylose and amylopectin biosynthesis. This study will aid the understanding of the spatiotemporal patterns and genetic regulation of endosperm development in different types of maize and provide valuable genetic information for the breeding of starch varieties with different contents.https://peerj.com/articles/7528.pdfGene expressionMaizeStarch metabolismRNA-sequenceEndosperm |
| spellingShingle | Jianzhou Qu Shutu Xu Xiaokang Tian Ting Li Licheng Wang Yuyue Zhong Jiquan Xue Dongwei Guo Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents PeerJ Gene expression Maize Starch metabolism RNA-sequence Endosperm |
| title | Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents |
| title_full | Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents |
| title_fullStr | Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents |
| title_full_unstemmed | Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents |
| title_short | Comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents |
| title_sort | comparative transcriptomics reveals the difference in early endosperm development between maize with different amylose contents |
| topic | Gene expression Maize Starch metabolism RNA-sequence Endosperm |
| url | https://peerj.com/articles/7528.pdf |
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