Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil
Hepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here, the HAV genotypes were analyzed of 18 isolates circulating between 199...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2008-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800015 |
| _version_ | 1797721191817936896 |
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| author | Fabíola Souza Fiaccadori Maristela Pereira Alexandre Siqueira Guedes Coelho Ana Maria Tavares Borges Juliana Alves Parente Célia Maria de Almeida Soares Divina das Dôres de Paula Cardoso |
| author_facet | Fabíola Souza Fiaccadori Maristela Pereira Alexandre Siqueira Guedes Coelho Ana Maria Tavares Borges Juliana Alves Parente Célia Maria de Almeida Soares Divina das Dôres de Paula Cardoso |
| author_sort | Fabíola Souza Fiaccadori |
| collection | DOAJ |
| description | Hepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here, the HAV genotypes were analyzed of 18 isolates circulating between 1996-2001 in Goiânia, state of Goiás, Brazil. Viral RNA was extracted from 18 serum samples and amplified (RT-PCR/nested-PCR), followed by the genomic sequencing of the VP1/2A junction region of the HAV genome. Sequences of 168 nucleotides were compared and analyzed using the BLAST N, Clustal X and PAUP v. 4.10b programs. All samples were classified as genotype I, with 10 belonging to subgenotype IA and eight to subgenotype IB. The subgenotype IA isolates showed greater diversity than the subgenotype IB isolates at the nucleotide level. Elevated identity values were found between isolates obtained in this study and those from other regions of the world, including Brazil, highlighting the high conservation among different isolates of this virus. However, changes in the HAV subgenotype circulation could also be observed during the evaluated period. |
| first_indexed | 2024-03-12T09:30:19Z |
| format | Article |
| id | doaj.art-57014df915554c5780b2237c1b8a4cb4 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:30:19Z |
| publishDate | 2008-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-57014df915554c5780b2237c1b8a4cb42023-09-02T13:54:26ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602008-12-01103883183510.1590/S0074-02762008000800015Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, BrazilFabíola Souza FiaccadoriMaristela PereiraAlexandre Siqueira Guedes CoelhoAna Maria Tavares BorgesJuliana Alves ParenteCélia Maria de Almeida SoaresDivina das Dôres de Paula CardosoHepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here, the HAV genotypes were analyzed of 18 isolates circulating between 1996-2001 in Goiânia, state of Goiás, Brazil. Viral RNA was extracted from 18 serum samples and amplified (RT-PCR/nested-PCR), followed by the genomic sequencing of the VP1/2A junction region of the HAV genome. Sequences of 168 nucleotides were compared and analyzed using the BLAST N, Clustal X and PAUP v. 4.10b programs. All samples were classified as genotype I, with 10 belonging to subgenotype IA and eight to subgenotype IB. The subgenotype IA isolates showed greater diversity than the subgenotype IB isolates at the nucleotide level. Elevated identity values were found between isolates obtained in this study and those from other regions of the world, including Brazil, highlighting the high conservation among different isolates of this virus. However, changes in the HAV subgenotype circulation could also be observed during the evaluated period.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800015acute hepatitisnucleotide sequencingsubgenotype IAsubgenotype IB |
| spellingShingle | Fabíola Souza Fiaccadori Maristela Pereira Alexandre Siqueira Guedes Coelho Ana Maria Tavares Borges Juliana Alves Parente Célia Maria de Almeida Soares Divina das Dôres de Paula Cardoso Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil Memorias do Instituto Oswaldo Cruz acute hepatitis nucleotide sequencing subgenotype IA subgenotype IB |
| title | Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil |
| title_full | Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil |
| title_fullStr | Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil |
| title_full_unstemmed | Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil |
| title_short | Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil |
| title_sort | molecular characterization of hepatitis a virus isolates from goiania goias brazil |
| topic | acute hepatitis nucleotide sequencing subgenotype IA subgenotype IB |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000800015 |
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