Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease
Blister blight (BB) disease is caused by the obligate biotrophic fungal pathogen <i>Exobasidium vexans</i> Massee and seriously affects the yield and quality of <i>Camellia sinensis</i>. The use of chemical pesticides on tea leaves substantially increases the toxic risks of t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/12/10225 |
| _version_ | 1797594272956940288 |
|---|---|
| author | Xiuju Yang Kunqian Cao Xiaoli Ren Guangyun Cao Weizhi Xun Jiayong Qin Xia Zhou Linhong Jin |
| author_facet | Xiuju Yang Kunqian Cao Xiaoli Ren Guangyun Cao Weizhi Xun Jiayong Qin Xia Zhou Linhong Jin |
| author_sort | Xiuju Yang |
| collection | DOAJ |
| description | Blister blight (BB) disease is caused by the obligate biotrophic fungal pathogen <i>Exobasidium vexans</i> Massee and seriously affects the yield and quality of <i>Camellia sinensis</i>. The use of chemical pesticides on tea leaves substantially increases the toxic risks of tea consumption. Botanic fungicide isobavachalcone (IBC) has the potential to control fungal diseases on many crops but has not been used on tea plants. In this study, the field control effects of IBC were evaluated by comparison and in combination with natural elicitor chitosan oligosaccharides (COSs) and the chemical pesticide pyraclostrobin (Py), and the preliminary action mode of IBC was also investigated. The bioassay results for IBC or its combination with COSs showed a remarkable control effect against BB (61.72% and 70.46%). IBC, like COSs, could improve the disease resistance of tea plants by enhancing the activity of tea-plant-related defense enzymes, including polyphenol oxidase (PPO), catalase (CAT), phenylalanine aminolase (PAL), peroxidase (POD), superoxide dismutase (SOD), <i>β</i>-1,3-glucanase (Glu), and chitinase enzymes. The fungal community structure and diversity of the diseased tea leaves were examined using Illumina MiSeq sequencing of the internal transcribed spacer (ITS) region of the ribosomal rDNA genes. It was obvious that IBC could significantly alter the species’ richness and the diversity of the fungal community in affected plant sites. This study broadens the application range of IBC and provides an important strategy for the control of BB disease. |
| first_indexed | 2024-03-11T02:21:19Z |
| format | Article |
| id | doaj.art-5701e8a8065d4000a5c9084880b65773 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:21:19Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5701e8a8065d4000a5c9084880b657732023-11-18T10:50:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121022510.3390/ijms241210225Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight DiseaseXiuju Yang0Kunqian Cao1Xiaoli Ren2Guangyun Cao3Weizhi Xun4Jiayong Qin5Xia Zhou6Linhong Jin7National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaNational Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaBlister blight (BB) disease is caused by the obligate biotrophic fungal pathogen <i>Exobasidium vexans</i> Massee and seriously affects the yield and quality of <i>Camellia sinensis</i>. The use of chemical pesticides on tea leaves substantially increases the toxic risks of tea consumption. Botanic fungicide isobavachalcone (IBC) has the potential to control fungal diseases on many crops but has not been used on tea plants. In this study, the field control effects of IBC were evaluated by comparison and in combination with natural elicitor chitosan oligosaccharides (COSs) and the chemical pesticide pyraclostrobin (Py), and the preliminary action mode of IBC was also investigated. The bioassay results for IBC or its combination with COSs showed a remarkable control effect against BB (61.72% and 70.46%). IBC, like COSs, could improve the disease resistance of tea plants by enhancing the activity of tea-plant-related defense enzymes, including polyphenol oxidase (PPO), catalase (CAT), phenylalanine aminolase (PAL), peroxidase (POD), superoxide dismutase (SOD), <i>β</i>-1,3-glucanase (Glu), and chitinase enzymes. The fungal community structure and diversity of the diseased tea leaves were examined using Illumina MiSeq sequencing of the internal transcribed spacer (ITS) region of the ribosomal rDNA genes. It was obvious that IBC could significantly alter the species’ richness and the diversity of the fungal community in affected plant sites. This study broadens the application range of IBC and provides an important strategy for the control of BB disease.https://www.mdpi.com/1422-0067/24/12/10225isobavachalconeblister blight diseasecontrol efficacydefensive enzymesmicrobial diversityinternal transcribed spacer (ITS) |
| spellingShingle | Xiuju Yang Kunqian Cao Xiaoli Ren Guangyun Cao Weizhi Xun Jiayong Qin Xia Zhou Linhong Jin Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease International Journal of Molecular Sciences isobavachalcone blister blight disease control efficacy defensive enzymes microbial diversity internal transcribed spacer (ITS) |
| title | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
| title_full | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
| title_fullStr | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
| title_full_unstemmed | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
| title_short | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
| title_sort | field control effect and initial mechanism a study of isobavachalcone against blister blight disease |
| topic | isobavachalcone blister blight disease control efficacy defensive enzymes microbial diversity internal transcribed spacer (ITS) |
| url | https://www.mdpi.com/1422-0067/24/12/10225 |
| work_keys_str_mv | AT xiujuyang fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT kunqiancao fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT xiaoliren fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT guangyuncao fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT weizhixun fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT jiayongqin fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT xiazhou fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease AT linhongjin fieldcontroleffectandinitialmechanismastudyofisobavachalconeagainstblisterblightdisease |