Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR
Stress levels inflicted by microcystin-LR extracted from Microcystis aeruginosa were assessed by estimating the effects on the oxidative stress biomarkers in the liver, muscle and gill tissues of Clarias gariepinus. Microcystin-LR was administered to the fish by injection into their intra-peritoneal...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-03-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853516302049 |
| _version_ | 1818477526964502528 |
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| author | Patrick Omoregie Isibor |
| author_facet | Patrick Omoregie Isibor |
| author_sort | Patrick Omoregie Isibor |
| collection | DOAJ |
| description | Stress levels inflicted by microcystin-LR extracted from Microcystis aeruginosa were assessed by estimating the effects on the oxidative stress biomarkers in the liver, muscle and gill tissues of Clarias gariepinus. Microcystin-LR was administered to the fish by injection into their intra-peritoneal cavity. Microcystin-LR induced the activities of mainly Glutathione-S-Transferase (GST), Catalase (CAT) and Superoxide dismutase (SOD) in the liver on dose and temporal basis, while it inhibited the activities of Cytochrome P450 on temporal basis in the liver and gill. Results show that MC-LR significantly induced oxidative stress in the C. gariepinus. Findings show that 400 μg MC-LR/kg b.w. disrupted the antioxidant status of the fish without any external physical manifestation on the body. Microcystin-LR induced the activities of mainly GST, CAT and SOD in the liver on dose and temporal basis while it inhibited the activities of CYP450 on temporal basis in the liver and gill. The adopted MC-LR doses; particularly 400 μgMC-LR/kg b.w. inflicted stress on the fish without any external/physical manifestation. MC-LR impacted on the antioxidant enzymes in the fish; in the order of liver > gill > muscle. This research has provided empirical evidences that 400 μg MC-LR/kg b.w induced oxidative stress in C. gariepinus in less than a month of exposure. This adopted level of MC-LR can be used as a warning signal in natural water bodies and commercial C. gariepinus ponds. |
| first_indexed | 2024-12-10T09:38:49Z |
| format | Article |
| id | doaj.art-5702089824d848deba289ea2272a9856 |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-10T09:38:49Z |
| publishDate | 2017-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-5702089824d848deba289ea2272a98562022-12-22T01:54:05ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352017-03-0161697510.1016/j.bjbas.2017.01.005Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LRPatrick Omoregie IsiborStress levels inflicted by microcystin-LR extracted from Microcystis aeruginosa were assessed by estimating the effects on the oxidative stress biomarkers in the liver, muscle and gill tissues of Clarias gariepinus. Microcystin-LR was administered to the fish by injection into their intra-peritoneal cavity. Microcystin-LR induced the activities of mainly Glutathione-S-Transferase (GST), Catalase (CAT) and Superoxide dismutase (SOD) in the liver on dose and temporal basis, while it inhibited the activities of Cytochrome P450 on temporal basis in the liver and gill. Results show that MC-LR significantly induced oxidative stress in the C. gariepinus. Findings show that 400 μg MC-LR/kg b.w. disrupted the antioxidant status of the fish without any external physical manifestation on the body. Microcystin-LR induced the activities of mainly GST, CAT and SOD in the liver on dose and temporal basis while it inhibited the activities of CYP450 on temporal basis in the liver and gill. The adopted MC-LR doses; particularly 400 μgMC-LR/kg b.w. inflicted stress on the fish without any external/physical manifestation. MC-LR impacted on the antioxidant enzymes in the fish; in the order of liver > gill > muscle. This research has provided empirical evidences that 400 μg MC-LR/kg b.w induced oxidative stress in C. gariepinus in less than a month of exposure. This adopted level of MC-LR can be used as a warning signal in natural water bodies and commercial C. gariepinus ponds.http://www.sciencedirect.com/science/article/pii/S2314853516302049Microcystin-LRMicrocystis aeruginosaOxidative stressHepatotoxinClarias gariepinus |
| spellingShingle | Patrick Omoregie Isibor Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR Beni-Suef University Journal of Basic and Applied Sciences Microcystin-LR Microcystis aeruginosa Oxidative stress Hepatotoxin Clarias gariepinus |
| title | Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR |
| title_full | Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR |
| title_fullStr | Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR |
| title_full_unstemmed | Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR |
| title_short | Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR |
| title_sort | oxidative stress biomarkers in clarias gariepinus burchel 1822 exposed to microcystin lr |
| topic | Microcystin-LR Microcystis aeruginosa Oxidative stress Hepatotoxin Clarias gariepinus |
| url | http://www.sciencedirect.com/science/article/pii/S2314853516302049 |
| work_keys_str_mv | AT patrickomoregieisibor oxidativestressbiomarkersinclariasgariepinusburchel1822exposedtomicrocystinlr |