Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection
As an antibody-free sensing membrane for the detection of the antibiotic tetracycline (TC), a liquid PVC membrane doped with the ion-pair tetracycline/θ-shaped anion [3,3′-Co(1,2-C<sub>2</sub>B<sub>9</sub>H<sub>11</sub>)<sub>2</sub>]<sup>−</su...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/1/71 |
| _version_ | 1797445333579464704 |
|---|---|
| author | Hamdi Ben Halima Abdoullatif Baraket Clara Vinas Nadia Zine Joan Bausells Nicole Jaffrezic-Renault Francesc Teixidor Abdelhamid Errachid |
| author_facet | Hamdi Ben Halima Abdoullatif Baraket Clara Vinas Nadia Zine Joan Bausells Nicole Jaffrezic-Renault Francesc Teixidor Abdelhamid Errachid |
| author_sort | Hamdi Ben Halima |
| collection | DOAJ |
| description | As an antibody-free sensing membrane for the detection of the antibiotic tetracycline (TC), a liquid PVC membrane doped with the ion-pair tetracycline/θ-shaped anion [3,3′-Co(1,2-C<sub>2</sub>B<sub>9</sub>H<sub>11</sub>)<sub>2</sub>]<sup>−</sup> ([<i>o</i>-COSAN]<sup>−</sup>) was formulated and deposited on a SWCNT modified gold microelectrode. The chosen transduction technique was electrochemical impedance spectroscopy (EIS). The PVC membrane was composed of: the tetracycline/[<i>o</i>-COSAN]<sup>−</sup> ion-pair, a plasticizer. A detection limit of 0.3 pg/L was obtained with this membrane, using bis(2-ethylhexyl) sebacate as a plasticizer. The sensitivity of detection of tetracycline was five times higher than that of oxytetracycline and of terramycin, and 22 times higher than that of demeclocycline. A shelf-life of the prepared sensor was more than six months and was used for detection in spiked honey samples. These results open the way to having continuous monitoring sensors with a high detection capacity, are easy to clean, avoid the use of antibodies, and produce a direct measurement. |
| first_indexed | 2024-03-09T13:25:15Z |
| format | Article |
| id | doaj.art-5702e7fa765a463e9dfd8b558a0b46da |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T13:25:15Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-5702e7fa765a463e9dfd8b558a0b46da2023-11-30T21:25:05ZengMDPI AGBiosensors2079-63742022-12-011317110.3390/bios13010071Selective Antibody-Free Sensing Membranes for Picogram Tetracycline DetectionHamdi Ben Halima0Abdoullatif Baraket1Clara Vinas2Nadia Zine3Joan Bausells4Nicole Jaffrezic-Renault5Francesc Teixidor6Abdelhamid Errachid7Institut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, FranceInstitut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, FranceInorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, Bellaterra, 08193 Barcelona, SpainInstitut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, FranceInstitute of Microelectronics of Barcelona (IMB-CNM, CSIC), Campus UAB, Bellaterra, 08193 Barcelona, SpainInstitut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, FranceInorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, Bellaterra, 08193 Barcelona, SpainInstitut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, FranceAs an antibody-free sensing membrane for the detection of the antibiotic tetracycline (TC), a liquid PVC membrane doped with the ion-pair tetracycline/θ-shaped anion [3,3′-Co(1,2-C<sub>2</sub>B<sub>9</sub>H<sub>11</sub>)<sub>2</sub>]<sup>−</sup> ([<i>o</i>-COSAN]<sup>−</sup>) was formulated and deposited on a SWCNT modified gold microelectrode. The chosen transduction technique was electrochemical impedance spectroscopy (EIS). The PVC membrane was composed of: the tetracycline/[<i>o</i>-COSAN]<sup>−</sup> ion-pair, a plasticizer. A detection limit of 0.3 pg/L was obtained with this membrane, using bis(2-ethylhexyl) sebacate as a plasticizer. The sensitivity of detection of tetracycline was five times higher than that of oxytetracycline and of terramycin, and 22 times higher than that of demeclocycline. A shelf-life of the prepared sensor was more than six months and was used for detection in spiked honey samples. These results open the way to having continuous monitoring sensors with a high detection capacity, are easy to clean, avoid the use of antibodies, and produce a direct measurement.https://www.mdpi.com/2079-6374/13/1/71tetracyclineelectrochemical impedance spectroscopysingle-walled carbon nanotubestetracycline/[<i>o</i>-COSAN]<sup>−</sup> ion-pair complexpolyvinyl chlorideselectivity |
| spellingShingle | Hamdi Ben Halima Abdoullatif Baraket Clara Vinas Nadia Zine Joan Bausells Nicole Jaffrezic-Renault Francesc Teixidor Abdelhamid Errachid Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection Biosensors tetracycline electrochemical impedance spectroscopy single-walled carbon nanotubes tetracycline/[<i>o</i>-COSAN]<sup>−</sup> ion-pair complex polyvinyl chloride selectivity |
| title | Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection |
| title_full | Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection |
| title_fullStr | Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection |
| title_full_unstemmed | Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection |
| title_short | Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection |
| title_sort | selective antibody free sensing membranes for picogram tetracycline detection |
| topic | tetracycline electrochemical impedance spectroscopy single-walled carbon nanotubes tetracycline/[<i>o</i>-COSAN]<sup>−</sup> ion-pair complex polyvinyl chloride selectivity |
| url | https://www.mdpi.com/2079-6374/13/1/71 |
| work_keys_str_mv | AT hamdibenhalima selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT abdoullatifbaraket selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT claravinas selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT nadiazine selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT joanbausells selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT nicolejaffrezicrenault selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT francescteixidor selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection AT abdelhamiderrachid selectiveantibodyfreesensingmembranesforpicogramtetracyclinedetection |