Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412
The thermophilic spore-forming strain Geobacillus sp. CX412 was isolated from hot spring soil in Tengchong City, Yunnan Province, China. We sequenced the complete genome of Geobacillus sp. CX412 using PacBio SMRT Sequencing. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) re...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1035311/full |
| _version_ | 1811318361036947456 |
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| author | Xin Li Wei Zhang Wei Zhang Xin-Ru Zhong Hao-Xuan Han Bin Dong Bin Dong |
| author_facet | Xin Li Wei Zhang Wei Zhang Xin-Ru Zhong Hao-Xuan Han Bin Dong Bin Dong |
| author_sort | Xin Li |
| collection | DOAJ |
| description | The thermophilic spore-forming strain Geobacillus sp. CX412 was isolated from hot spring soil in Tengchong City, Yunnan Province, China. We sequenced the complete genome of Geobacillus sp. CX412 using PacBio SMRT Sequencing. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results indicated that Geobacillus sp. CX412 is a novel species in the genus Geobacillus. The metabolic potential of Geobacillus sp. CX412 based on COG, KEGG, and CAZymes analysis demonstrated that Geobacillus sp. CX412 was a highly adaptable strain with an unusually high number of 73 annotated transposons in the genome, which is relatively rare in Geobacillus. Compared with the near-derived strains, it was found that Geobacillus sp. CX412 has the unique β-lactam resistance and more active metabolism (more than 50.5–100.1%). Additionally, its genome encodes glycoside hydrolases and other genes related to lignocellulose breakdown, suggesting that Geobacillus sp. CX412 has a considerable biomass degradation potential. Thus, Geobacillus sp. CX412 is a new thermophilic bacterial species that add to the increasing repertoire of known lignocellulose degraders. |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T12:24:12Z |
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| spelling | doaj.art-5702ee6c98b741c08924661fd195c76a2022-12-22T02:47:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10353111035311Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412Xin Li0Wei Zhang1Wei Zhang2Xin-Ru Zhong3Hao-Xuan Han4Bin Dong5Bin Dong6School of Environmental Science and Engineering, Tongji University, Shanghai, ChinaSchool of Environmental Science and Engineering, Tongji University, Shanghai, ChinaSchool of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai, ChinaSchool of Environmental Science and Engineering, Tongji University, Shanghai, ChinaSchool of Environmental Science and Engineering, Tongji University, Shanghai, ChinaSchool of Environmental Science and Engineering, Tongji University, Shanghai, ChinaYANGTZE Eco-Environment Engineering Research Center, China Three Gorges Corporation, Beijing, ChinaThe thermophilic spore-forming strain Geobacillus sp. CX412 was isolated from hot spring soil in Tengchong City, Yunnan Province, China. We sequenced the complete genome of Geobacillus sp. CX412 using PacBio SMRT Sequencing. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results indicated that Geobacillus sp. CX412 is a novel species in the genus Geobacillus. The metabolic potential of Geobacillus sp. CX412 based on COG, KEGG, and CAZymes analysis demonstrated that Geobacillus sp. CX412 was a highly adaptable strain with an unusually high number of 73 annotated transposons in the genome, which is relatively rare in Geobacillus. Compared with the near-derived strains, it was found that Geobacillus sp. CX412 has the unique β-lactam resistance and more active metabolism (more than 50.5–100.1%). Additionally, its genome encodes glycoside hydrolases and other genes related to lignocellulose breakdown, suggesting that Geobacillus sp. CX412 has a considerable biomass degradation potential. Thus, Geobacillus sp. CX412 is a new thermophilic bacterial species that add to the increasing repertoire of known lignocellulose degraders.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1035311/fullGeobacillus sp. CX412thermophiletransposonsβ-lactam resistancelignocellulose |
| spellingShingle | Xin Li Wei Zhang Wei Zhang Xin-Ru Zhong Hao-Xuan Han Bin Dong Bin Dong Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412 Frontiers in Microbiology Geobacillus sp. CX412 thermophile transposons β-lactam resistance lignocellulose |
| title | Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412 |
| title_full | Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412 |
| title_fullStr | Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412 |
| title_full_unstemmed | Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412 |
| title_short | Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412 |
| title_sort | genome sequencing analysis of a novel thermophilic strain geobacillus sp cx412 |
| topic | Geobacillus sp. CX412 thermophile transposons β-lactam resistance lignocellulose |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1035311/full |
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