Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress
Polyphenols sourced from <i>Ilex latifolia</i> Thunb. (PIT) contain high levels of phenolic acids, tannic acids, triterpenoids and so on, which play important roles in antioxidant function. This study was conducted to investigate the effects of PIT against intestinal injury in piglets un...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/966 |
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| author | Xiao Xu Yu Wei Hongwei Hua Xiaoqing Jing Huiling Zhu Kan Xiao Jiangchao Zhao Yulan Liu |
| author_facet | Xiao Xu Yu Wei Hongwei Hua Xiaoqing Jing Huiling Zhu Kan Xiao Jiangchao Zhao Yulan Liu |
| author_sort | Xiao Xu |
| collection | DOAJ |
| description | Polyphenols sourced from <i>Ilex latifolia</i> Thunb. (PIT) contain high levels of phenolic acids, tannic acids, triterpenoids and so on, which play important roles in antioxidant function. This study was conducted to investigate the effects of PIT against intestinal injury in piglets under oxidative stress. Thirty-two weanling piglets were arranged by a 2 × 2 factorial experiment with diets (basal diet vs. PIT diet) and oxidative stress (saline vs. diquat). All piglets were injected with saline or diquat on d 21, respectively. After 7 days, all pigs were slaughtered and intestinal samples were collected. PIT enhanced jejunal villus heights and crypt depth in the piglets under oxidative stress. PIT increased the activities of intestinal mucosal lactase, sucrase and maltase in the challenged piglets. PIT also increased the jejunal ratio of protein to DNA and ileal protein content. PIT increased the jejunal activities of GSH-PX and GSH content and reduced the ileal MDA amounts. Furthermore, PIT regulated the expression of ferroptosis mediators, such as <i>TFR1</i>, <i>HSPB1</i>, <i>SLC7A11</i> and <i>GPX4</i>. These results indicate that dietary PIT supplementation enhances the histological structure and function of the intestinal mucosa, which is involved in modulating antioxidant capacity and ferroptosis. |
| first_indexed | 2024-03-10T03:26:17Z |
| format | Article |
| id | doaj.art-5704481f58be439aaa4e4bcd6eb98376 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:17Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5704481f58be439aaa4e4bcd6eb983762023-11-23T09:52:24ZengMDPI AGAntioxidants2076-39212022-05-0111596610.3390/antiox11050966Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative StressXiao Xu0Yu Wei1Hongwei Hua2Xiaoqing Jing3Huiling Zhu4Kan Xiao5Jiangchao Zhao6Yulan Liu7Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaPolyphenols sourced from <i>Ilex latifolia</i> Thunb. (PIT) contain high levels of phenolic acids, tannic acids, triterpenoids and so on, which play important roles in antioxidant function. This study was conducted to investigate the effects of PIT against intestinal injury in piglets under oxidative stress. Thirty-two weanling piglets were arranged by a 2 × 2 factorial experiment with diets (basal diet vs. PIT diet) and oxidative stress (saline vs. diquat). All piglets were injected with saline or diquat on d 21, respectively. After 7 days, all pigs were slaughtered and intestinal samples were collected. PIT enhanced jejunal villus heights and crypt depth in the piglets under oxidative stress. PIT increased the activities of intestinal mucosal lactase, sucrase and maltase in the challenged piglets. PIT also increased the jejunal ratio of protein to DNA and ileal protein content. PIT increased the jejunal activities of GSH-PX and GSH content and reduced the ileal MDA amounts. Furthermore, PIT regulated the expression of ferroptosis mediators, such as <i>TFR1</i>, <i>HSPB1</i>, <i>SLC7A11</i> and <i>GPX4</i>. These results indicate that dietary PIT supplementation enhances the histological structure and function of the intestinal mucosa, which is involved in modulating antioxidant capacity and ferroptosis.https://www.mdpi.com/2076-3921/11/5/966antioxidative capacityferroptosisgene expressionhistological structureintestinal mucosaoxidative stress |
| spellingShingle | Xiao Xu Yu Wei Hongwei Hua Xiaoqing Jing Huiling Zhu Kan Xiao Jiangchao Zhao Yulan Liu Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress Antioxidants antioxidative capacity ferroptosis gene expression histological structure intestinal mucosa oxidative stress |
| title | Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress |
| title_full | Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress |
| title_fullStr | Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress |
| title_full_unstemmed | Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress |
| title_short | Polyphenols Sourced from <i>Ilex latifolia</i> Thunb. Relieve Intestinal Injury via Modulating Ferroptosis in Weanling Piglets under Oxidative Stress |
| title_sort | polyphenols sourced from i ilex latifolia i thunb relieve intestinal injury via modulating ferroptosis in weanling piglets under oxidative stress |
| topic | antioxidative capacity ferroptosis gene expression histological structure intestinal mucosa oxidative stress |
| url | https://www.mdpi.com/2076-3921/11/5/966 |
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